Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Beet root (Beta vulgaris) protects lipopolysaccharide and alcohol-induced liver damage in rat

  • Kim, Bae-Hwan (Department of Public Health, Keimyung University) ;
  • Jung, Se-Hoon (Department of Public Health, Keimyung University) ;
  • Jung, Suryun (Department of Public Health, Keimyung University)
  • Received : 2019.05.17
  • Accepted : 2019.11.27
  • Published : 2020.07.15
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Abstract

The purpose of this study was to investigate the protective effects of beet root (Beta vulgaris var. rubra) in lipopolysaccharide (LPS) and alcohol induced liver damage. Beta vulgaris ethanol extract (BVEE) showed good antioxidant activity in the contents of polyphenol and flavonoid compounds, and the electron-donating ability and ABTS+ radical scavenging activity. As for anti-inflammatory effect in RAW 264.7 cells, inhibition rate of nitric oxide production was increased in dose dependent manner. In hepatotoxicity model induced by LPS and alcohol in rat, BVEE significantly decreased serum AST, ALT and γ-GTP concentrations in a dose-dependent manner. The histopathological changes after H&E staining showed that fat accumulation and inflammatory cell infiltration were decreased by BVEE. The collagen fibers around the central lobule observed by Masson's trichrome staining were also decreased by BVEE. In addition, as for the immunohistochemical staining and Transmission electron microscopy, BVEE improved morphological characteristics of damaged liver lesion. The increased mRNA expressions of NF-κB, MAPK1, MAPK3, CYP2E1, and α-SMA were significantly decreased in BVEE treated group. These results indicated that BVEE would have protective effects in hepatotoxicity by altering various indicators related to the liver damage induced by LPS or alcohol.

Keywords

References

  1. O'shea RS, Dasarathy S, McCullough AJ (2010) Alcoholic liver disease. Hepatology 51:307-328 https://doi.org/10.1002/hep.23258
  2. Friedman SL (2003) Liver fibrosis from bench to bedside. J Hepatol 38:38-53 https://doi.org/10.1016/S0168-8278(02)00429-4
  3. Jeong WI, Park O, Gao B (2008) Abrogation of the antifibrotic effects of natural killer cells/interferon-gamma contributes to alcohol acceleration of liver fibrosis. Gastroenterology 134:248-258 https://doi.org/10.1053/j.gastro.2007.09.034
  4. Lucas K, Maes M (2013) Role of the Toll Like receptor (TLR) radical cycle in chronic inflammation: possible treatments targeting the TLR4 pathway. Mol Neurobiol 48:190-204 https://doi.org/10.1007/s12035-013-8425-7
  5. Mandrekar P, Szabo G (2009) Signalling pathways in alcoholinduced liver inflammation. J Hepatol 50:1258-1266 https://doi.org/10.1016/j.jhep.2009.03.007
  6. Lee HJ (2010) Update and perspectives on alcoholic liver disease in Korea 2010. Clin Mol Hepatol 16:23-44
  7. Lichtenthaler R, Marx F (2005) Total oxidant scavenging capacities of common European fruit and vegetable juices. Food Chem 53:103-110 https://doi.org/10.1021/jf0307550
  8. Junyan Han J, Zhang Z, Yang S, Wang J, Yang X, Tan D (2014) Betanin attenuates paraquat-induced liver toxicity through a mitochondrial pathway. Food Chem Toxicol 70:100-106 https://doi.org/10.1016/j.fct.2014.04.038
  9. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200 https://doi.org/10.1038/1811199a0
  10. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  11. Folin O, Denis W (1912) On phosphotungstic-phosphomolybdic compounds as color regents. J Biol Chem 12:239-243 https://doi.org/10.1016/S0021-9258(18)88697-5
  12. Guzik TJ, Korbut R, Adamek-Guzik T (2003) Nitric oxide superoxide in inflammation and immune regulation. J Physiol Phamacol 43:469-487
  13. Yang X, Kang MC, Lis Y, Kim EA, Kang SM, Jeon YJ (2014) Anti-inflammatory activity of questinol isolated from marine-derived fungus eurotium amstelodami in lipopolysaccharide-stimulated raw 264.7 macrophages. J Korean Med Sci 8:367-373 https://doi.org/10.3346/jkms.1993.8.5.367
  14. Kim EJ, Choi JY, Yu MR, Kim YM, Lee SH, Lee BH (2012) Total polyphenols, total favonoid contents, and antioxidant activity of Korean natural and medicinal plants. J Food Sci Technol 44:337-342
  15. Stewart S, Jones D, Day CP (2001) Alcoholic liver disease: new insights into mechanisms and preventative strategies. Trends Mol Med 7:408-413 https://doi.org/10.1016/S1471-4914(01)02096-2
  16. Wang Q, Dai X, Yang W, Wang H, Zhao H, Yang F, Yang Y, Li J, Lv X (2015) Caffeine protects against alcohol-induced liver fibrosis by dampening the cAMP/PKA/CREB pathwayin rat hepatic stellate cells. Int Immunopharmacol 25:340-352 https://doi.org/10.1016/j.intimp.2015.02.012
  17. Koo SW, Lee KW (2012) The protective effect of ginger aqueous extracts on CCl4-induced hepatic damage in mice. J Vet Clin 29:441-446
  18. Li W, Zhu C, Li Y, Wu Q, Gao R (2014) MEST attenuates CCl4-induced liver fibrosis in rats by inhibiting the wnt/β-catenin signaling pathway. Gut Liver 8:282-291 https://doi.org/10.5009/gnl.2014.8.3.282
  19. Duarte Rojo A, Ruiz Margain A, Macias Rodriguez RU, Cubero FJ, Estradas Trujillo J, Munoz Fuentes RM, Torre A (2016) Clinical scenarios for the use of S100β as a marker of hepatic encephalopathy. World J Gstroenterol 22:4397-4402 https://doi.org/10.3748/wjg.v22.i17.4397
  20. Li CH, Piao DM, Xu WX, Yin ZR, Jin JS, Shen ZS (2005) Morphological and serum hyaluronic acid, laminin and type IV collagen changes in dimethylnitrosamine-induced hepatic fibrosis of rats. World J Gastroenterol 11:7620-7624 https://doi.org/10.3748/wjg.v11.i48.7620
  21. Miyazawa Y, Fukuda Y, Imoto M, Koyama Y, Nagura H (1988) Immunohistochemical studies on the distribution of nerve fibers in chronic liver diseases. Am J Gastroenterol 83:1108-1114
  22. Sternlieb I, Quintana N (1977) The peroxisomes of human hepatocytes. Lab Investig 36:140-149
  23. Zhao M, Howard EW, Parris AB, Guo Z, Zhao Q, Yang X (2017) Alcohol promotes migration and invasion of triple-negative breast cancer cells through activation of p38 MAPK and JNK. Mol Carcinog 56:849-862 https://doi.org/10.1002/mc.22538
  24. Chang X, Luo F, Jiang W, Zhu L, Gao J, He H, Wei T, Gong S, Yan T (2015) Protective activity of salidroside against ethanol-induced gastric ulcer via the MAPK/NF-κB pathway in vivo and in vitro. Int Immunopharmacol 28:604-615 https://doi.org/10.1016/j.intimp.2015.07.031
  25. Xu J, Zhao Y, Aisa HA (2017) Anti-inflammatory effect of pomegranate flower in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Pharm Biol 55:2095-2101 https://doi.org/10.1080/13880209.2017.1357737
  26. Barnes PJ, Karin M (1997) Nuclear factor κB-a pivotal factor in chronic inflammatory diseases. N Engl J Med 336:1066-1071 https://doi.org/10.1056/NEJM199704103361506
  27. Dela Pena A, Leclercq I, Field J, George J, Jones B, Farrell G (2005) NFkappaB activation, rather than TNF, mediates hepatic inflammation in a murine dietary model of steatohepatitis. Gastroenterology 129:1663-1674 https://doi.org/10.1053/j.gastro.2005.09.004
  28. Majdalawieh A, Ro HS (2010) Regulation of IkappaBalpha function and NF-kappaB signaling: AEBP1 is a novel proinflammatory mediator in macrophages. Mediat Inflamm 2010:823821 https://doi.org/10.1155/2010/823821
  29. Ha KT, Yoon SJ, Choi DY, Kim DW, Kim JK, Kim CH (2005) Protective effect of lycium chinense fruit on carbon tetrachloride-induced hepatotoxicity. J Ethnopharmacol 96:529-535 https://doi.org/10.1016/j.jep.2004.09.054
  30. Yu ES, Choe GY, Gong GG, Lee IC (1993) Expression of alpha-smooth muscle actin in liver disease. J Korean Med Sci 8:367-373 https://doi.org/10.3346/jkms.1993.8.5.367
  31. Park SL, Lee SY, Kim IS, Lim SI, Choi JS, Choi SY (2013) Hepatoprotective activity of quality certificated traditional doenjang in korea. Food Eng Prog 17:401-406 https://doi.org/10.13050/foodengprog.2013.17.4.401

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