Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Action mechanism of anti-wrinkle effect of Rhamnus yoshinoi methanol extract in human dermal fibroblast and keratinocyte cell lines

  • Kim, Hyun Ok (Department of Public Health, Graduate School, Keimyung University) ;
  • Shin, Kyoung Ran (Department of Molecular Medicine, College of Medicine, Keimyung University) ;
  • Jang, Byeong-Churl (Department of Molecular Medicine, College of Medicine, Keimyung University) ;
  • Kim, Young Chul (Department of Public Health, Graduate School, Keimyung University)
  • Received : 2018.10.16
  • Accepted : 2019.04.25
  • Published : 2020.01.15
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Abstract

Rhamnus yoshinoi is a deciduous broad-leaf bush and endemic species widely found in Korea. Recently, we reported that R. yoshinoi methanol extract (RYME) had excellent antioxidant activity and inhibition of collagenase and elastase activity in cell-free system. In this study, we investigated the ability of RYME to control the mRNA and protein expression levels of the known skin wrinkle-related factors in cultured human dermal fibroblast and keratinocyte cell lines. Treatment with 100 or 200 ㎍/mL RYME strongly blocked the UVB-induced downregulation of type 1 collagen mRNA expression (p < 0.001) and partially blocked the UVB-induced upregulation of MMP-3 mRNA expression in HaCaT human keratinocytes (p < 0.05 or p < 0.001). Treatment with RYME at 100 ㎍/mL considerably decreased MMP-1 mRNA expression in UVB-exposed HaCaT cells (p < 0.01). In HaCaT cells, RYME exhibited the potential to improve UV light-induced skin wrinkles. Moreover, RYME selectively inhibited the UVB-induced ERK-1/2 protein phosphorylation in CCD-986sk human dermal fibroblasts at 80 and 160 ㎍/mL. UV-induced ERK-1/2 protein phosphorylation is one of the major mechanisms of the generation of UV-induced skin wrinkles. Therefore, it is likely that the anti-skin wrinkling effect of RYME could be attributable to selective inhibition of UV induced ERK-1/2 protein phosphorylation.

Keywords

References

  1. Brohem CA, Cardeal LB, Tiago M, Soengas MS, Barros SB, Maria-Engler SS (2011) Artificial skin in perspective: concepts and applications. Pigment Cell Melanoma Res 24:35-50 https://doi.org/10.1111/j.1755-148X.2010.00786.x
  2. Martin P (1997) Wound healing-aiming for perfect skin regeneration. Science 276:75-81 https://doi.org/10.1126/science.276.5309.75
  3. Majeed M, Bhat B, Anand S, Sivakumar A, Paliwal P, Geetha KG (2011) Inhibition of UV-induced ROS and collagen damage by Phyllanthus emblica extract in normal human dermal fibroblasts. J Cosmet Sci 62:49-56
  4. Quan T, He T, Kang S, Voorhees JJ, Fisher GJ (2004) Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-β type II receptor/smad signaling. Am J Pathol 165:741-751 https://doi.org/10.1016/S0002-9440(10)63337-8
  5. Nelson AR, Fingleton B, Rothenberg ML, Matrisian LM (2000) Matrix metalloproteinases: biologic activity and clinical implications. J Clin Oncol 18:1135-1149 https://doi.org/10.1200/JCO.2000.18.5.1135
  6. Park CH, Chung JH (2011) Epidermal growth factor-induced matrix metalloproteinase-1 expression is negatively regulated by p38 MAPK in human skin fibroblasts. J Dermatol Sci 64:134-141 https://doi.org/10.1016/j.jdermsci.2011.07.002
  7. Overall CM (2001) Matrix metalloproteinase substrate binding domains, modules and exosites: overview and experimental strategies. Methods Mol Biol 151:79-120
  8. Xu Y, Fisher GJ (2005) Ultraviolet (UV) light irradiation induced signal transduction in skin photoaging. J Dermatol Sci 1:51-58
  9. Karin M, Hunter T (1995) Transcriptional control by protein phosphorylation: signal transmission form the cell surface nucleus. Curr Biol 5:747-757 https://doi.org/10.1016/S0960-9822(95)00151-5
  10. Angel P, Szabowski A, Schorpp-Kistner M (2001) Function and regulation of AP-1 subunits in skin physiology and pathology. Oncogene 20:2413-2423 https://doi.org/10.1038/sj/onc/1204380
  11. Jung HY, Shin JC, Park SM, Kim NR, Kwak W, Choi BH (2014) Pinus densiflora extract protects human skin fibroblasts against UVB-induced photoaging by inhibiting the expression of MMPs and increasing type I procollagen expression. Toxicol Rep 1:658-666 https://doi.org/10.1016/j.toxrep.2014.08.010
  12. Kim HO, Oh CJ, Jang B, Kim YC (2018) Antioxidant activity and anti-wrinkle effect of Rhamnus yoshinoi methanol extract. J Invest Cosmetol 14:421-427 https://doi.org/10.15810/JIC.2018.14.4.003
  13. Bosch R, Philips N, Suarez-Perez JA, Juarranz A, Devmurari A, Chalensouk-Khaosaat J, Gonzalez S (2015) Mechanisms of photoaging and cutaneous photocarcinogenesis, and photoprotective strategies with phytochemicals. Antioxidants 4:248-268 https://doi.org/10.3390/antiox4020248
  14. Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, Voorhees JJ (1996) Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379:335-339 https://doi.org/10.1038/379335a0
  15. Brenneisen P, Wenk J, Klotz LO, Wlaschek M, Briviba K, Krieg T, Sies H, Scharffetter-Kochanek K (1998) Central role of ferrous/ferric iron in the ultraviolet B irradiation-mediated signaling pathway leading to increased interstitial collagenase (matrixdegrading metalloprotease (MMP)-1) and stromelysin-1 (MMP-3) mRNA levels in cultured human dermal fibroblasts. J Biol Chem 273:5279-5287 https://doi.org/10.1074/jbc.273.9.5279
  16. Quan T, Qin Z, Xia W, Shao Y, Voorhees JJ, Fisher GJ (2009) Matrix-degrading metalloproteinases in photoaging. J Investig Dermatol Symp Proc 14:20-24 https://doi.org/10.1038/jidsymp.2009.8
  17. Feng XX, Yu XT, Li WJ, Kong SZ, Liu YH, Zhang X, Xian YF, Zhang XJ, Su ZR, Lin ZX (2014) Effects of topical application of patchouli alcohol on the UV-induced skin photoaging in mice. Eur J Pharm Sci 63:113-123 https://doi.org/10.1016/j.ejps.2014.07.001
  18. Scharffetter-Kochanek K, Brenneisen P, Wenk J, Herrmann G, Ma W, Kuhr L, Meewes C, Wlaschek M (2000) Photoaging of the skin from phenotype to mechanisms. Exp Gerontol 35:307-316 https://doi.org/10.1016/S0531-5565(00)00098-X
  19. Boukamp P, Petrussevska RT, Breitkreutz D, Hornung J, Markham A, Fusenig NE (1988) Normal keratinization in a spontaneously immortalized aneuploidy human keratinocyte cell line. J Cell Biol 106:761-771 https://doi.org/10.1083/jcb.106.3.761
  20. Blanpain C, Lowry WE, Geoghegan A, Polak L, Fuchs E (2004) Self-renewal, multipotency, and the existence of two cell populations within an epithelial stem cell niche. Cell 118:635-648 https://doi.org/10.1016/j.cell.2004.08.012
  21. Moon HJ, Park KS, Ku MJ, Lee MS, Jeong SH, Imbs TI, Zvyagintseva TN, Ermakova SP, Lee YH (2009) Effect of Costaria costata fucoidan on expression of matrix metalloproteinase-1 promoter, mRNA, and protein. J Nat Prod 72:1731-1734 https://doi.org/10.1021/np800797v
  22. Kim MS, Oh GH, Kim MJ, Hwang JK (2013) Fucosterol inhibits matrix metalloproteinase expression and promotes type I procollagen production in UVB-induced HaCaT cells. Photochem Photobiol 89:911-918 https://doi.org/10.1111/php.12061
  23. Sudel KM, Venzke K, Mielke H, Breitenbach U, Mundt C, Jaspers S, Koop U, Sauermann K, Knussmann-Hartig E, Moll L, Gercken G, Young AR, Stab F, Wenck H, Gallinat S (2005) Novel aspects of intrinsic and extrinsic aging of human skin: beneficial effects of soy extract. Photochem Photobiol Sci 81:581-587 https://doi.org/10.1562/2004-06-16-RA-202.1
  24. Inomata S, Matsunaga Y, Amano S, Takada K, Kobayashi K, Tsunenaga M, Nishiyama T, Kohno Y, Fukuda M (2003) Possible involvement of gelatinases in basement membrane damage and wrinkle formation in chronically ultraviolet B-exposed hairless mouse. J Invest Dermatol 120:128-134 https://doi.org/10.1046/j.1523-1747.2003.12021.x
  25. Robinson MJ, Cobb MH (1997) Mitogen-activated protein kinase pathways. Curr Opin Cell Biol 9:180-186 https://doi.org/10.1016/S0955-0674(97)80061-0
  26. Rosette C, Karin M (1996) Ultraviolet light and osmotic stress: activation of the JNK cascade through multiple growth factor and cytokine receptors. Science 274:1194-1197 https://doi.org/10.1126/science.274.5290.1194
  27. Gum R, Wang H, Lengyel E, Juarez J, Boyd D (1997) Regulation of 92 kDa type IV collagenase expression of the jun aminoterminal kinase- and the extracellular signal-regulated kinase-dependent signaling cascades. Oncogene 14:1481-1493 https://doi.org/10.1038/sj/onc/1200973
  28. Chen A, Davis BH (1999) UV irradiation activates JNK and increases αI(I) collagen gene expression in rat hepatic stellate cells. J Biol Chem 274:158-164 https://doi.org/10.1074/jbc.274.1.158
  29. Xia Z, Dicknens M, Raingeand J, Davs RJ, Greenberg ME (1995) Opposing effects of ERK and JNK-p38 MAP kinase on apoptosis. Science 270:1326-1331 https://doi.org/10.1126/science.270.5240.1326
  30. Ham J, Babij C, Whitfield J, Pfarr CM, Lallemand D, Yaniv M, Rubin LL (1995) A c-Jun dominant negative mutant protects sympathetic neurons against programmed cell death. Neuron 14:927-939 https://doi.org/10.1016/0896-6273(95)90331-3
  31. Rittie L, Fisher GJ (2002) UV-light-induced signal cascades and skin aging. Ageing Res Rev 1:705-720 https://doi.org/10.1016/S1568-1637(02)00024-7

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