한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제28권5호
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  • Pages.339-346
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  • 2020
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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천궁 돌연변이 유발을 위한 최적 감마선 조사량

Gamma-ray Irradiation on Radio Sensitivity in Cnidium officinale Makino

  • 정진태 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 하보근 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 한종원 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 이정훈 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 이상훈 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 오명원 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 박춘근 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 마경호 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 장재기 (국립원예특작과학원 인삼특작부 약용작물과) ;
  • 김상훈 (한국원자력연구원 첨단방사선연구소 방사선육종연구실) ;
  • 김진백 (한국원자력연구원 첨단방사선연구소 방사선육종연구실) ;
  • 강시용 (공주대학교 산업과학대학 원예학과) ;
  • 류재혁 (한국원자력연구원 첨단방사선연구소 방사선육종연구실)
  • Jeong, Jin Tae (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Ha, Bo Keun (Division of Plant Biotechnology, Chonnam National University) ;
  • Han, Jong Won (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Jeong Hoon (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Sang Hoon (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Oh, Myeong Won (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Park, Chun Geon (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Ma, Kyung Ho (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Chang, Jae Ki (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Sang Hoon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si Yong (Department of Horticulture, College of Industrial Sciences, Kongju National University) ;
  • Ryu, Jai Hyunk (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 투고 : 2020.06.11
  • 심사 : 2020.09.29
  • 발행 : 2020.10.30
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초록

Background: Cnidium officinale Makino have been used in traditional medicine in Northeast Asia. Although gamma-ray mutagenesis has been used to develop breeding resources with novel characteristics, research on the radiation sensitivity of C. officinale Makino is limited. Hence, the optimal gamma-ray dosage for mutation breeding in C. officinale Makino was investigated. Methods and Results: Seedstocks were exposed to doses of gamma rays (5 Gy - 50 Gy), and subsequently planted in a greenhouse. After 30 days of sowing, the survival rates and growth decreased rapidly at doses above 20 Gy, while all individuals died at 50 Gy. The median lethal dose (LD50) was 25.65 Gy, and the median reduction doses (RD50) for plant height, number of stems, and fresh weight were 12.81, 9.32, and 23.26 Gy, respectively. Post-irradiation levels of malondialdehyde (MDA), peroxidase (POD), and chlorophyll in the aerial parts of the plant were quantified using spectrophotometry. Relative to the controls, the levels of MDA and POD increased, while the level of chlorophyll decreased at doses ≥ 10 Gy, indicating cellular damage. Conclusions: A dose of 20 Gy was found to be optimal for mutation breeding in C. officinale Makino.

키워드

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