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Quality Characteristics and Antioxidant Activity of Puffed Rice Vinegar Added with Lemon Balm Extracts

레몬밤 추출물을 함유한 팽화미 식초의 품질 특성 및 항산화 활성

  • Received : 2020.09.22
  • Accepted : 2020.10.06
  • Published : 2020.10.30

Abstract

To enhance the usability of puffed rice, we evaluated the quality characteristics and antioxidant activity of vinegar made with puffed rice and lemon balm. First, puffed rice containing lemon balm extracts was fermented for alcohol production. The alcoholic fermented liquid was adjusted to an alcohol content of 6% and an initial acidity of 2% for vinegar fermentation. The pH and alcohol content of the lemon balm vinegar decreased overall as fermentation progressed. The total acidity was 5.20±0.01% (2.0% lemon balm) to 5.80±0.01% (0.0% lemon balm) on the 15th day of fermentation, and the total acidity was slightly lower as the amount of added lemon balm was increased. Citric, succinic, and acetic organic acids were detected in the vinegar and the acetic acid content was highest at 4.81% to 5.37% on the 15th day of fermentation. We found that the total polyphenol and total flavonoid content of the lemon balm vinegar increased as we added lemon balm, and the content on the 15th day of fermentation was 179.4±4.5 (0.0% lemon balm) - 340.8±2.6 mg/kg (2.0% lemon balm) and 1.5±0.3 (0.0% lemon balm) - 31.7±0.3 mg/kg (2.0% lemon balm), respectively. The higher the amount of lemon balm added, the higher the DPPH radical scavenging activity, the FRAP (reducing power), and the ABTS radical scavenging activity. Moreover, those activities after 2.0% lemon balm treatment were 82.99±0.61%, 1.08±0.01 abs., and 87.67±0.68%, respectively, on the 15th day of fermentation. These results suggest that vinegar with added lemon balm may be used as an antioxidant agent.

당화공정이 간편한 장점이 있는 팽화미의 이용성을 높이기 위하여 팽화미를 당화한 후 항산화 효과가 좋은 레몬밤을 첨가하여 알코올 발효를 하고, 이를 이용하여 제조한 식초의 품질 특성과 항산화 활성을 조사하였다. 효소로 당화한 팽화미에 레몬밤 추출물을 농도별로 첨가하여 Saccharomyces cerevisiae KCCM 11201로 알코올 발효 후 알코올 함량 6% 및 초기 산도 2%로 조절한 다음, Acetobacter aceti KCCM 40229로 30℃, 200 rpm로 15일간 초산 발효를 하여 식초를 제조하였다. 팽화미 식초의 pH 및 알코올 함량은 발효가 진행되면서 전체적으로 감소하였고, 총산은 발효 15일째 5.20±0.01%(레몬밤 2.0%)-5.80±0.01%(레몬밤 0.0%)로 레몬밤 첨가량이 증가할수록 총산이 약간 낮게 나타났다. 팽화미 식초의 유기산 함량은 발효 15일에 5.26%(레몬밤 2.0%)-5.97%(레몬밤 0.0%)이었으며, acetic acid의 함량이 가장 높았다. 팽화미 식초의 총 폴리페놀 함량과 총 플라보노이드 함량은 레몬밤 첨가량이 많을수록 함량이 높았으며, 발효 15일에 각각 179.4±4.5 mg/kg(레몬밤 0.0%)-340.8±2.6 mg/kg(레몬밤 2.0%) 및 1.5±0.3 mg/kg(레몬밤 0.0%)-31.7±0.3 mg/kg(레몬밤 2.0%)이었다. 레몬밤 첨가량이 많을수록 DPPH 라디칼 소거 활성, FRAP(환원력) 및 ABTS 라디칼 소거 활성이 높게 나타나 레몬밤 2.0% 처리구는 발효 15일에 각각 82.99±0.61%, 1.08±0.01 abs.(at 593 nm), 87.67±0.68%를 나타냈다. 본 연구결과 레몬밤을 첨가한 팽화미 식초가 항산화 활성을 갖는 식초로 활용될 수 있는 가능성이 있다는 것을 확인하였다.

Keywords

References

  1. Hoeiuxhi, J.I., Kanno, T., Kobayashi, M., Effective onion vinegar production by a two-step fermentation system. J. Biosci. Bioeng., 90, 289-293 (2000). https://doi.org/10.1016/S1389-1723(00)80083-3
  2. Ministry of Food and Drug Safety, 2019. Food Code, Korean Food Industry Association. Kyeongsung Munhwasa. Seoul, Korea. pp. 163-164.
  3. Lim, J.H., Jeong, S.W., Park, K.J., Cho, M.H., Joo, K.H., Effect of fermentation method and brown rice content on quality characteristics of brown rice vinegar. Korean J. Food Preserv., 16, 33-39 (2009).
  4. Yoon, S.R., Kim, G.R., Lee, J.H., Lee, S.W., Yeo, S.H., Jeong, Y.J., Kwon, J.H., Properties of organic acids and volatile components in brown rice vinegar prepared using different yeasts and fermentation methods. Korean J. Food Preserv., 17, 733-740 (2010).
  5. Yoon, H.N., Chemical characterization of commercial vinegars. Korean J. Food Sci. Technol., 31, 1440-1446 (1999).
  6. Park, C.S., Kim, K.S., Noh, J.G., Rho, C.W., Yoon, H.S., Quality characteristics of the germinated brown rice vinegar added with red pepper. Korea Soc. Food Sci. Nutr., 39, 567-572 (2010). https://doi.org/10.3746/jkfn.2010.39.4.567
  7. Jeong, Y.J., Seo, J.H., Jung, S.H., Shin, S.R., Kim, K.S., The quality comparison of uncleaned rice vinegar by two stages fermentation with commercial uncleaned rice vinegar. Korean J. Postharvest Sci. Technol., 5, 374-379 (1998).
  8. Kim, Y.D., Kang, S.H., Kang, S.K., Studies on the acetic acid fermentation using maesil juice. Korean Soc. Food Sci. Nutr., 25, 695-700 (1996).
  9. Ministry of Agriculture, Food and Rural Affairs, 2019. The principal statistics of agriculture, forestry and fishery. Hanla Plan, Sejong, Korea. pp. 307-310.
  10. Kim, D.H., 2010. Food Chemistry, Tamgudang publishing Co., Seoul. Korea. pp. 418-419.
  11. National Tax Service Technical Service Institute, 2005. Manufacturing guideline of takju and yakju, Seoul, Korea. pp. 31, 53-54, 195-196.
  12. Sung, Y.K., Changes in physicochemical characteristics of puffed rice powder added kochujang. Master thesis, Seoul National University of Science and Technology, Seoul, Korea. pp. 1-2 (2012).
  13. Cho, T.D., 2005. Herb Story, Sallimbooks, Paju, Korea, pp. 42-43.
  14. Kim, I.J., Lee, J.K., Park, M.H., Shon, D.H., Ha, Y.L., Ryu, C.H., Preparation method of meju by three step fermentation. Korean. J. Food Sci. Technol., 34, 536-539 (2002).
  15. Kim, Y.S., Jeong, D.Y., Shin, D.H., Optimum fermentation conditions and fermentation characteristics of mulberry (Morus alba) wine. Korean J. Food Sci. Technol., 40, 63-69 (2008).
  16. National Tax Service Institute, 2010. National Tax Service: Analysis regulation of liquor. Seoul, Korea, pp. 39-40.
  17. Bois, M.S., Antioxidant determination by the use of a stable free radical. Nature, 26, 1199-1204 (1958).
  18. Dewanto, V., Wu, X., Adonm, K.K., Liu, R.H., Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agric. Food Chem., 50, 3010-3014 (2002). https://doi.org/10.1021/jf0115589
  19. Jia, Z., Tang, M., Wu, J., The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 64, 555-559 (1999). https://doi.org/10.1016/S0308-8146(98)00102-2
  20. Benzie, I.F., Strain, J.J., The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem., 239, 70-76 (1996). https://doi.org/10.1006/abio.1996.0292
  21. Arts, M.J.T.J., Haenen, G.R.M.M., Voss, H.P., Bast, A., Antioxidant capacity of reaction products limits the applicability of the trolox equivalent antioxidant capacity (TEAC) assay. Food Chem. Toxicol., 42, 45-49 (2004). https://doi.org/10.1016/j.fct.2003.08.004
  22. Jeong, P.H., Kim, Y.S., Shin, D.H., Changes of physicochemical characteristics of Schzandra chinensis during postharvest ripening at various temperature. Korean J. Food Sci. Technol., 38, 469-474 (2006).
  23. SAS. SAS User Guide; SAS/STAT, Version 6. SAS Inst Inc, Cary, NC. (1990).
  24. Lee, S.W., Kwon, J.H., Yoon, S.R., Woo, S.M., Yeo, S.H., Jeong, Y.J., Quality characteristics of brown rice vinegar prepared using varying amounts of nuruk (an amylolytic enzyme preparation) and employing different fermentation conditions. Korean J. Food Preserv., 18, 26-32 (2011). https://doi.org/10.11002/kjfp.2011.18.1.026
  25. Kim, G.R., Yoon, S.R., Lee, J.H., Yeo, S.H., Jeong, Y.J., Yoon, K.Y., Kwon, J.H., Physicochemical properties of and volatile components in commercial fruit vinegars. Korean J. Food Preserv., 17, 616-624 (2010).
  26. Woo, S.M., Jo, J.Y., Lee, S.W., Kwon, J.H., Yeo, S.H., Jeong, Y.J., Quality comparison of static-culture and commercial brown rice vinegars. Korean J. Food Preserv., 19, 301-307 (2012). https://doi.org/10.11002/kjfp.2012.19.2.301
  27. Keum, J.H., Studies on garlic and pumpkin vinegar. Korean J. Food Nutr., 12, 518-522 (1999).
  28. Moon, S.Y., Chung, H.C., Yoon, H.N., Comparative analysis of commercial vinegars in physicochemical properties, minor components and organoleptic tastes. Korean J. Food Sci. Technol., 29, 663-670 (1997).
  29. Lee S.W., Kwon, J.H., Yoon, S.R., Woo, S.M., Jang, S.Y., Yeo, S.H., Choi, J.H., Jeong, Y.J., Quality characteristics of brown rice vinegar by different yeasts and fermentation condition. J. Korean Soc. Food Sci. Nutr., 39, 1366-1372 (2010). https://doi.org/10.3746/jkfn.2010.39.9.1366
  30. Kim, G.R., Yoon, S.R., Lee, J.H., Yeo, S.H., Jeong, Y.J., Yoon, K.Y., Kwon, J.H., Physicochemical properties of and volatile components in commercial fruit vinegars. Korean J. Food Preserv., 17, 616-624 (2010).
  31. Chung, N.H., Jo, Y.H., Gao, Y., Gu, S.Y., Jeong, Y.J., Kwon, J.H., Comparison of physicochemical properties and antioxidant activities of naturally fermented commercial rice vinegars produced in Korea, China, and Japan. J. Korean Soc. Food Sci. Nutr., 44, 1799-1805 (2015). https://doi.org/10.3746/jkfn.2015.44.12.1799
  32. Na, H.S., Choi, G.C., Yang, S.I., Lee, J.H., Cho, J.Y., Ma, S.J., kim, J.Y., Comparison of characteristics in commercial fermented vinegars made with different ingredients. Korean J. Food Preserv., 20, 482-487 (2013). https://doi.org/10.11002/kjfp.2013.20.4.482
  33. Park, S.Y., Chae, K.S., Son, R.H., Jung, J.H., Im, Y.R., Kwon, J.W., Quality characteristics and antioxidant activity of bokbunja (black raspberry) vinegars. Food Eng. Prog., 16, 340-346 (2012).
  34. Bang, S.I., Gwon, G.H., Cho, E.J., Lee, A.Y., Seo, W.T., Characteristics of fermented vinegar using mulberry and its antioxidant activity. Korean J. Food Preserv., 27, 651-662 (2020). https://doi.org/10.11002/kjfp.2020.27.5.651
  35. Shin, J.Y., Kang, M.J., Kang, J.R., Choi, J.S., Quality characteristics of fermented vinegar containing different concentration of an ethanol extract from 'Seomaeyaksuk' (Artemisia argyi H.). Korean J. Food Preserv., 27, 212-223 (2020). https://doi.org/10.11002/kjfp.2020.27.2.212
  36. Hong, S.M., Moon, H.S., Lee, J.H., Lee, H.I., Jeong, J.H., Lee, M.K., Seo, K.I., Development of functional vinegar by using cucumbers. J. Korean Soc. Food Sci. Nutr., 41, 927-935 (2012). https://doi.org/10.3746/jkfn.2012.41.7.927
  37. Lee, S.W., Quality characteristics and fermentation process establishment of brown-rice vinegar prepared by alcohol and vinegar fermentation conditions. Doctor's thesis, Kyungpook National University, Daegu, Korea. pp. 90-91 (2012).
  38. Lee, S.M., Choi, Y.M., Kim, Y.W., Kim, D.J., Lee, J.S., Antioxidant activity of vinegars commercially available in Korean markets. Food Eng. Prog., 13, 221-225 (2009).