Journal of the Korean Ceramic Society (한국세라믹학회지)

  • Volume 59
  • /
  • Pages.527-535
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  • 2020
  • /
  • 1229-7801(pISSN)
  • /
  • 2234-0491(eISSN)
The Korean Ceramic Society (한국세라믹학회)
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Reusable and rapid esterolysis of nitrophenyl alkanoates with CalB enzyme-immobilized magnetic nanoparticles

  • Ha Yull Lee (Center for Convergence Bioceramic Materials, Korea Institute of Ceramic Engineering and Technology (KICET)) ;
  • Woo Young Jang (Center for Convergence Bioceramic Materials, Korea Institute of Ceramic Engineering and Technology (KICET)) ;
  • Jeong Ho Chang (Center for Convergence Bioceramic Materials, Korea Institute of Ceramic Engineering and Technology (KICET))
  • Received : 2021.10.18
  • Accepted : 2021.12.15
  • Published : 2022.02.18
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Abstract

This study reports the preparation of the Candida antarctica lipase B (CalB) enzyme immobilization on silica-coated magnetic nanoparticles (Si-MNPs@CalB) using various cross-linkers and demonstration of rapid catalytic hydrolysis of p-nitrophenyl alkyl esters. CalB enzymes were coupled with different cross-linker silanes on the Si-MNPs surface. Among these cross-linkers, Cl-functionalized silane was better at immobilization of CalB than the others. Catalytic hydrolysis of p -nitrophenyl alkyl esters was demonstrated against Si-MNPs@CalB as a function of the length of alkyl chain (C4, C8, C12, and C16). From the Michaelis-Menten equation and Lineweaver-Burk plots, various enzyme kinetic parameters (i.e., Km, Vmax, and Kcat) were calculated. Catalytic hydrolysis was faster in shorter alkyl chain of p-nitrophenyl alkyl esters with Si-MNPs@CalB in the order C4>>C8>C12>>C16. Furthermore, the reusability and optimum catalytic activity of Si-MNPs@ CalB were evaluated as a function of the number of reuses and with different pH values.

Keywords

Acknowledgement

This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project no. PJ0149382020)" Rural Development Administration, Republic of Korea.