DOI QR코드

DOI QR Code

성페로몬을 이용한 열대거세미나방 포획과 시토크롬 옥시다제 1(CO1)에서 종내 변이군 특이적 단일염기다형성 분포

Sex Pheromone Trapping of Spodoptera frugiperda (Noctuidae: Lepidoptera) in Korea and the Distribution of Intraspecies-specific Single Nucleotide Polymorphisms in the Cytochrome c Oxidase Subunit 1 (CO1)

  • 서보윤 (국립농업과학원 작물보호과) ;
  • 정진교 (국립식량과학원 재배환경과) ;
  • 이관석 (국립농업과학원 작물보호과) ;
  • 양창열 (국립원예특작과학원 원예특작환경과) ;
  • 조점래 (국립농업과학원 작물보호과) ;
  • 김양표 (고창군 농업기술센터)
  • Seo, Bo Yoon (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Jung, Jin Kyo (Crop Cultivation and Environment Research Division, National Institute of Crop Science) ;
  • Lee, Gwan-Seok (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Yang, Chang Yeol (Horticultural and Herbal Crop Environment Research Division, National Institute of Horticultural and Herbal Science) ;
  • Cho, Jumrae (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kim, Yang Pyo (Gochang-gun Agricultural Extension)
  • 투고 : 2020.05.13
  • 심사 : 2020.08.13
  • 발행 : 2020.09.01

초록

2019년에 서남해안 지역인 고창군의 옥수수 밭 주변에서 성페로몬을 이용하여 열대거세미나방(Spodoptera frugiperda) 성충을 효과적으로 모니터링하는 방법을 조사하였다. 총 함량이 300 또는 1000 ㎍인 2종류 성분 조성의 성페로몬 미끼[(100%) (Z)-9-tetradecenyl acetate and (2%) (Z)-7-dodecenyl acetate]를 설치한 깔대기형 트랩과 델타형 트랩 중에서 열대거세미나방은 300 ㎍ 미끼의 깔대기형 트랩에서 8월 6일에 처음 잡혔고 가장 많이 포획되었다. 또한 깔대기형 트랩 모두에서 비표적 종인 뒷흰가는줄무늬밤나방(Mythimna loreyi)이 많이 포획되었다. 총 함량이 1000 ㎍인 위의 2종류 성분 조성과 4종류 성분 조성의 미끼[(100%) (Z)-9-tetradecenyl acetate, (8%) (Z)-11-hexadecenyl acetate, (2%) (Z)-7-dodecenyl acetate, and (1%) (Z)-9-dodecenyl acetate]를 설치한 날개형 트랩에서 열대거세미나방은 비슷한 수준의 낮은 포획수를 보였으나 뒷흰가는줄무늬밤나방은 4종류 성분 조성의 미끼에서 훨씬 더 많이 포획되었다. 성페로몬 트랩에 포획된 열대거세미나방 70마리의 미토콘드리아 시토크롬 옥시다제 1(CO1)의 부분염기서열(1,004 bp)을 이용하여 계통수를 분석한 결과, 두 개의 종내 변이군으로 나눠졌으며 66마리가 CO1-RS로, 나머지 4마리는 CO1-CS로 분지되었다. 또한 두 개의 CO1 변이군과 기주식물계통(벼, 옥수수)에서 일관되게 차이가 있는 총 12개의 CO1 단일염기다형성(SNP)이 확인되었으며, 전체 73마리 중 4마리만 CO1-CS 그룹(옥수수계통 포함)과 동일한 패턴을 보였으며 나머지 69마리는 CO1-RS그룹(벼계통 포함)과 같았다.

In 2019, the sex pheromones of Spodoptera frugiperda were used to examine moth trapping in cornfields in Gochang, Korea. Four types of traps were prepared, two funnel-types and two delta-types, each baited with 300 or 1000 ㎍ of a two-component (2C) blend of synthetic sex pheromones [100% (Z)-9-tetradecenyl acetate (Z9-14Ac) and 2% (Z)-7-dodecenyl acetate (Z7-12Ac)]. The greatest number of S. frugiperda were captured in the 300 ㎍ funnel-type trap (first catch: August 6). Large numbers of Mythimna loreyi (a non-target) were also caught in the funnel-type traps. Two wing-type traps were baited with 1000 ㎍ of the 2C blend or a four-component (4C) blend [100% (Z)-9-tetradecenyl acetate, 8% (Z)-11-hexadecenyl acetate (Z11-16Ac), 2% (Z)-7-dodecenyl acetate, and 1% (Z)-9-dodecenyl acetate (Z9-12Ac)] and the capture efficiency was assessed. Low numbers of S. frugiperda were captured regardless of the blend, and more M. loreyi were captured using the 4C blend. The two intraspecies groups clustered separately in a phylogenetic tree constructed using partial sequences (1004 bp) of cytochrome c oxidase subunit 1 (CO1). Of the 70 S. frugiperda captured in the pheromone traps, 66 belonged to CO1-RS (CO1 rice-strain) and 4 to CO1-CS (CO1 corn-strain). Twelve consistent single nucleotide polymorphisms (SNPs) were identified in CO1 between the CO1-RS and CO1-CS groups of S. frugiperda. Of the 73 S. frugiperda, 4 had the same SNP pattern as the CO1-CS group (including the corn strain) and 69 had the same SNP pattern as the CO1-RS group (including the rice strain).

키워드

참고문헌

  1. Andrade, R., Rodriguez, C., Oehlschlager, A.C., 2000. Optimization of a pheromone lure for Spodoptera frugiperda (Smith) in Central America. J. Braz. Chem. Soc. 11, 609-613. https://doi.org/10.1590/S0103-50532000000600009
  2. Batista-Pereira, L.G., Stein, K., De Pauls, A.F., Moreira, J.A., Cruz, I., 2006. Isolation, identification, synthesis, and field evaluation of the sex pheromone of the Brazilian population of Spodoptera frugiperda. J. Chem. Ecol. 32, 1085-1099. https://doi.org/10.1007/s10886-006-9048-5
  3. Dumas, P., Legeai, F., Lemaitre, C., Scaon, E., Orsucci, M., Labadie, K., Gimenez, S., Clamens, A., Henri, H., Vavre, F., Aury, J., Fournier, P., Kergoat, G.J., d'Alencon, E., 2015. Spodoptera frugiperda (Lepidoptera: Noctuidae) host-plant variants: Two host strains or two distinct species? Genetica 143, 305-316. https://doi.org/10.1007/s10709-015-9829-2
  4. Fleischer, S.J., Harding, C.L., Blom, P.E., White, J., Grehan, J., 2005. Spodoptera frugiperda pheromone lures to avoid nontarget captures of Leucania phragmatidicola. J. Econ. Entomol. 98, 66-71. https://doi.org/10.1093/jee/98.1.66
  5. Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R., 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol., Biotechnol. 3, 294-299.
  6. Goergen, G., Kumar, P.L., Sankung, S.B., Togola, A., Tamo, M., 2016. First report of outbreaks of the fall armyworm Spodoptera frugiperda (JE Smith) (Lepidoptera, Noctuidae), a new alien invasive pest in west and central Africa. PLoS ONE 11, e0165632. https://doi.org/10.1371/journal.pone.0165632
  7. Gouin, A., Bretaudeau, A., Nam, K., Gimenez, S., Aury, J., Duvic, B., Hilliou, F., Durand, N., Montagne, N., Darboux, I., Kuwar, S., Chertemps, T., Siaussat, D., Bretschneider, A., Mone, Y., Ahn, S., Hanniger, S., Grenet, A.G., Neunemann, D., Maumus, F., Luyten, I., Labadie, K., Xu, W., Koutroumpa, F., Escoubas, J., Llopis, A., Maibeche-Coisne, M., Salasc, F., Tomar, A., Anderson, A.R., Khan, S.F., Dumas, P., Orsucci, M., Guy, J., Belser, C., Alberti, A., Noel, B., Couloux, A., Mercier, J., Nidelet, S., Dubois, E., Liu, N., Boulogne, I., Mirabeau, O., Le Goff, G., Gordon, K., Oakeshott, J., Consoli, F.L., Volkoff, A., Fescemyer, H.W., Marden, J.H., Luthe, D.S., Herrero, S., Heckel, D.G., Wincker, P., Kergoat, G.J., Amselem, J., Quesneville, H., Groot, A.T., Jacquin-Joly, E., Negre, N., Lemaitre, C., Legeai, F., d'Alencon, E., Fournier, P., 2017. Two genomes of highly polyphagous lepidopteran pests (Spodoptera frugiperda, Noctuidae) with different host-plant ranges. Sci. Rep. 7, 11816. https://doi.org/10.1038/s41598-017-10461-4
  8. Groot, A.T., Marr, M., Schofl, G., Lorenz, S., Svatos, A., Heckel, D.G., 2008. Host strain specific sex pheromone variation in Spodoptera frugiperda. Front Zool. 5, 20. https://doi.org/10.1186/1742-9994-5-20
  9. Groot, A.T., Unbehend, M., Hanniger, S., Juarez, M.L., Kost, S., Heckel, D.G., 2016. Evolution of reproductive isolation of Spodoptera frugiperda. in: Allison, J.D., Carde, R.T. (Eds.), Pheromone communication in moths: Evolution, behavior, and application. University of California press, Oakland, pp. 291-300.
  10. Haenniger, S., Goergen, G., Akinbuluma, M.D., Kunert, M., Heckel, D.G., Unbehend, M., 2020. Sexual communication of Spodoptera frugiperda from West Africa: Adaptation of an invasive species and implications for pest management. Sci. Rep. 10, 2892. https://doi.org/10.1038/s41598-020-59708-7
  11. Ho, H.Y., Tsai, R.S., Hsu, E.L., Chow, Y.S., Kou, R., 2002. Investigation of possible sex pheromone components of female Loreyi leafworm, Acantholeucania loreyi (Duponchel) (Lepidoptera: Noctuidae) in Taiwan. Zool. Stud. 41, 188-193.
  12. Johnson, S.J., 1987. Migration and the life history strategy of the fall armyworm, Spodoptera frugiperda in the western hemisphere. Insect Sci. Applic. 8, 543-549.
  13. Juarez, M.L., Schofl, G., Vera, M.T., Vilardi, J.C., Murua, M.G., Willink, E., Hanniger, S., Heckel, D.G., Groot, A.T., 2014. Population structure of Spodoptera frugiperda maize and rice host forms in South America: are they host strains? Entomol. Exp. Appl. 152, 182-199. https://doi.org/10.1111/eea.12215
  14. Kergoat, G.J., Prowell, D.P., Le Ru, B.P., Mitchell, A., Dumas, P., Clamens, A.-L., Condamine, F.L., Silvain, J.-F., 2012. Disentangling dispersal, vicariance and adaptive radiation patterns: A case study using armyworms in the pest genus Spodoptera (Lepidoptera: Noctuidae). Mol. Phylogenet. Evol. 65, 855-870. https://doi.org/10.1016/j.ympev.2012.08.006
  15. Kumar, S., Stecher, G., Tamura, K., 2015. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33, 1870-1874. https://doi.org/10.1093/molbev/msw054
  16. Landolt, P.J., Smithhisler, C.L., 1998. Isolation and identification of female sex pheromone and development of a sex attraction for Lacanobia subjuncta. J. Chem. Ecol. 24, 1369-1379. https://doi.org/10.1023/A:1021230901040
  17. Lee, G.-S., Seo, B.Y., Lee, J., Kim, H., Song, J.H., Lee, W., 2020. First report of the fall armyworm, Spodoptera frugiperda (Smith, 1979) (Lepidoptera, Noctuidae), a new migratory pest in Korea. Korean J. Appl. Entomol. 59, 73-78. https://doi.org/10.5656/KSAE.2020.02.0.006
  18. Levy, H.C., Garcia-Maruniak, A., Maruniak, J.E., 2002. Strain identification of Spodoptera frugiperda (Lepidoptera: Noctuidae) insects and cell line: PCR-RFLP of cytochrome oxidase C subunit I gene. Fla. Entomol. 85, 186-190. https://doi.org/10.1653/0015-4040(2002)085[0186:SIOSFL]2.0.CO;2
  19. Li, X.-J., Wu, M.-F., Ma, J., Gao, B.-Y., Wu, Q.-L., Chen, A.-D., Liu, J., Jiang, Y.-Y., Zhai, B.-P., Early, R., Chapman, J.W., Hu, G., 2019. Prediction of migratory routes of the invasive fall armyworm in eastern China using a trajectory analytical approach. Pest Manag. Sci. 76, 454-463. https://doi.org/10.1002/ps.5530
  20. Lima, E.R., McNeil, J.N., 2009. Female sex pheromones in the host races and hybrids of the fall armyworm, Spodoptera fruigiperda (Lepidoptera: Noctuidae). Chemoecology. 19, 29-36. https://doi.org/10.1007/s00049-009-0005-y
  21. Ma, J., Wang, Y.-P., Wu, M.-F., Gao, B.-Y., Liu, J., Lee, G.-S., Otuka, A., Hu, G., 2019. High risk of the fall armyworm invading into Japan and the Korean peninsula via overseas migration. J. Appl. Entomol. 143, 911-920. https://doi.org/10.1111/jen.12679
  22. Malo, E.A., Bahena, F., Miranda, M.A., Valle-Mora, J., 2004. Factors affecting the trapping of males of Spodoptera frugiperda (Lepidoptera: Noctuidae) with pheromones in Mixico. Fla. Entomol. 87, 288-293. https://doi.org/10.1653/0015-4040(2004)087[0288:FATTOM]2.0.CO;2
  23. Meagher, R.L., Gallo-Meagher, M., 2003. Identifying host strains of fall armyworm (Lepidoptera: Noctuidae) in Florida using mitochondrial markers. Fla. Entomol. 86, 450-455. https://doi.org/10.1653/0015-4040(2003)086[0450:IHSOFA]2.0.CO;2
  24. Meagher, R.L., Nagoshi, R.N., 2013. Attraction of fall armyworm males (Lepidoptera: Noctuidae) to host strain females. Environ. Entomol. 42, 751-757. https://doi.org/10.1603/EN13007
  25. Meagher, R.L., Nagoshi, R.N., Armstrong, J.S., Niogret, J., Epsky, N., 2013. Captures and host strains of fall armyworm (Lepidoptera: Noctuidae) males in traps baited with different commercial pheromone blends. Fla. Entomol. 96, 729-740. https://doi.org/10.1653/024.096.0305
  26. Meagher Jr, R.L., Agboka, K., Tounou, A.K., Koffi, D., Agbevohia, K.A., Amouze, T.R., Adjevi, K.M., Nagoshi, R.N., 2019. Comparison of pheromone trap design and lures for Spodoptera frugiperda in Togo and genetic characterization of moths caught. Entomol. Exp. Appl. 167, 507-516. https://doi.org/10.1111/eea.12795
  27. Mitchell, E., Agee, H.R., Heath, R.R., 1989. Influence of pheromone trap color and design on capture of male velvetbean caterpillar and fall armyworm moths (Lepidoptera: Noctuidae). J. Chem. Ecol. 15, 1775-1784. https://doi.org/10.1007/BF01012265
  28. Mitchell, E., Tumlinson, J.H., McNeil, J.N., 1985. Field evaluation of commercial pheromone formulations and traps using a more effective sex pheromone blend for the fall armyworm (Lepidoptera: Noctuidae). J. Econ. Entoml. 78, 1364-1369. https://doi.org/10.1093/jee/78.6.1364
  29. Montezano, D.G., Specht, A., Sosa-Gomez, D.R., Roque-Specht, V.F., Sousa-Silva, J.C., Paula-Moraes, S.V., Peterson, J.A., Hunt, T.E., 2018. Host plants of Spodoptera frugiperda (Lepidoptera: Noctuidae) in the Americas. African Entomol. 26, 286-300. https://doi.org/10.4001/003.026.0286
  30. Nagoshi, R.N., 2010. The fall armyworm Triose phosphate isomerase (Tpi) gene as a marker of strain identify and interstrain mating. Ann. Entmol. Soc. Am. 103, 283-292. https://doi.org/10.1603/AN09046
  31. Nagoshi, R.N., 2012. Improvements in the identification of strains facilitate population studies of fall armyworm subgroups. Ann. Entomol. Soc. Am. 105, 351-358. https://doi.org/10.1603/AN11138
  32. Nagoshi, R.N., Goergen, G., Tounou, K.A., Agboka, K., Koffi, D., Meagher, R.L., 2018. Analysis of strain distribution, migratory potential, and invasion history of fall armyworm populations in northern Sub-Saharan Africa. Sci. Rep. 8, 3710. https://doi.org/10.1038/s41598-018-21954-1
  33. Nagoshi, R.N., Goergen, G., Du Plessis, H., van den Berg, J., Meagher Jr., R., 2019. Genetic comparisons of fall armyworm populations from 11 countries spanning sub-Saharan Africa provide insights into strain composition and migratory behaviors. Sci. Rep. 9, 8311. https://doi.org/10.1038/s41598-019-44744-9
  34. Nagoshi, R.N., Meagher, R.L., Hay-Roe, M., 2012. Inferring the annual migration patterns of the fall armyworm (Lepidoptera: Noctuidae) in the United States from mitochondrial haplotypes. Ecol. Evol. 2, 1458-1467. https://doi.org/10.1002/ece3.268
  35. Otim, M.H., Tay, W.T., Walsh, T.K., Kanyesigye, D., Adumo, S., Abongosi, J., Ochen, S., Sserumaga, J., Alibu, S., Abalo, G., Asea, G., Agona, A., 2018. Detection of sister-species in invasive populations of the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) from Uganda. PLoS ONE 13, e0194571. https://doi.org/10.1371/journal.pone.0194571
  36. Pashley, D.P., 1986. Host-associated genetic differentiation in fall armyworm (Lepidoptera: Noctuidae): A sibling species complex? Ann. Entomol. Soc. Am. 79, 898-904. https://doi.org/10.1093/aesa/79.6.898
  37. Pashley, D.P., 1988. Current status of fall armyworm host strains. Fla. Entomol. 71, 227-234. https://doi.org/10.2307/3495425
  38. Prowell, D.P., McMicheal, M., Silvain, J.F., 2004. Multilocus genetic analysis of host use, introgression, and speciation in host strains of fall armyworm (Lepidoptera: Noctuidae). Ann. Entomol. Soc. Am. 97, 1034-1044. https://doi.org/10.1603/0013-8746(2004)097[1034:MGAOHU]2.0.CO;2
  39. Qin, J. Zhang, L., Liu, Y., Sappington, T.W., Cheng, Y., Luo, L., Jiang, X., 2017. Population projection and development of the Mythimna loreyi (Lepidoptera: Noctuidae) as affected by temperature: Application of an age-stage, two-sex life table. J. Econ. Entomol. 110, 1583-1591. https://doi.org/10.1093/jee/tox138
  40. Seo, B.Y., Park, C.G., Koh, Y-.H., Jung, J.K., Cho, J., Kang, C., 2017. ITS2 DNA Sequence analysis for eight species of delphacid planthoppers and a loop-mediated isothermal amplification method for the brown planthopper-specific detection. Korean J. Appl. Entomol. 56, 377-385. https://doi.org/10.5656/KSAE.2017.10.0.033
  41. Sato, Y., Takahashi, S., Sakai, M., Kodama, T., 1980. Attractiveness of the synthetic sex pheromone to the males of the armyworm, Leucania separata Walker and the lorey leafworm Leucania loreyi Duponchel (Lepidoptera: Noctuidae). Appl. Entomol. Zool. 15, 334-340. https://doi.org/10.1303/aez.15.334
  42. Sparks, A.N., 1979. A review of the biology of the fall armyworm. Fla. Entomol. 62, 82-86. https://doi.org/10.2307/3494083
  43. Tumlinson, J.H., Mitchell, E.R., Teal, P.E.A., Heath, R.R., Mengelkoch, L.J., 1986. Sex pheromone of fall armyworm, Spodoptera frugiperda (J.E. SMITH). Identification of components critical to attraction in the field. J. Chem. Ecol. 12, 1909-1926. https://doi.org/10.1007/BF01041855
  44. Takahashi, S., Kawaradani, M., Sato, Y., Sakai, M., 1979. Sex pheromone compounds of Leucania separata Walker and Leucania loreyi Duponchel. Jpn. J. Appl. Entomol. Zool. 23, 78-81. https://doi.org/10.1303/jjaez.23.78
  45. Unbehend, M., Hanniger, S., Meagher, R.L., Heckel, D.G., Groot, A.T., 2013. Pheromonal divergence between two strains of Spodoptera frugiperda. J. Chem. Ecol. 39, 364-376. https://doi.org/10.1007/s10886-013-0263-6
  46. Unbehend, M., Hanniger, S., Vasquez, G.M., Juarez, M.L., Reisig, D., McNeil, J.N., Meagher, R.L., Jenkins, D.A., Heckel, D.G., Groot, A.T., 2014. Geographic variation in sexual attraction of Spodoptera frugiperda corn- and rice-strain males to pheromone lures. PLoS ONE 9, e89255. https://doi.org/10.1371/journal.pone.0089255