Journal of Haehwa Medicine (혜화의학회지)

Research Institute of Korean Medicine (대전대학교 한의학연구소)
권호 표지

Effects of herbal mixture on blood glucose and lipid metabolism in type 2 diabetic mellitus mouse

천연물 복합제가 제 2형 당뇨 마우스에서 혈당 및 지질대사에 미치는 영향

  • Seo, Dong-hyo (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Joo, In-Hwan (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
  • 서동효 (대전대학교 한의과대학 병리학교실) ;
  • 주인환 (대전대학교 한의과대학 병리학교실) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Published : 2020.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: This study aims to investigate the effects of diabetes mellitus care mixture (DCM) on blood glucose and lipid metabolism in type 2 diabetic mellitus mice. DCM consisted of lagerstroemia speciose, allium hookeri, momordica charantia, amaranthus tricolor, and boesenbergia rotunda, which have been proven to have antidiabetic properties. Methods: In this study, we researched the effects of DCM in type 2 diabetic mellitus mice. C57BLKS/J mouse groups had no treatment, db/db mouse randomly assigned to 2 groups, and treated with distilled water and DCM (200 mg/kg/day). Blood glucose levels and body weight were checked every week. After 4 weeks of treatment, liver function indicators (AST, ALT, and LDH) and lipid metabolites (triglyceride, total cholesterol, LDL-cholesterol, HDL-cholesterol) were measured with a biochemistry analyzer. Diabetic factors (insulin, resistin, and leptin) were measured with ELISA. Results: DCM was decreased blood glucose, diabetic factors, liver function indicators, triglyceride, total cholesterol, and LDL-cholesterol significantly. Also, HDL-cholesterol was significantly increased in DCM group. The bodyweight of DCM group decreased but, no significant difference with the control group. DCM may have the potential to improved diabetes mellitus by regulating blood glucose levels and diabetic factors. Also protecting from diabetic complications by adjusting liver function indicators and lipid metabolites. Conclusions: These results suggest that DCM to be used as an oriental medicine for diabetes, the results of clinical trials are needed.

Keywords

References

  1. Organization WH. World Health Organization Global Report on Diabetes. Geneva: World Health Organization. 2016.
  2. Lyon MF, Searle AG. Genetic variants and strains of the laboratory mouse: Oxford University Press; 1989.
  3. Kanasaki K, Koya D. Biology of obesity: lessons from animal models of obesity. BioMed Research International. 2011;2011.
  4. Fujita H, Fujishima H, Morii T, Koshimura J, Narita T, Kakei M, et al. Effect of metformin on adipose tissue resistin expression in db/db mice. Biochemical and biophysical research communications. 2002;298(3):345-9. https://doi.org/10.1016/S0006-291X(02)02464-6
  5. KH K. Hwang-je-nae-kyeong. Seoul: Banryong. 2000;77:120.
  6. Kim H-G, Bose S, Kim D-I, Koo B-S, Kim H. Effects of fermented lotus extracts on glucose intolerance and lipid metabolism-related gene expression. Journal of Korean Medicine Rehabilitation. 2014;24(1):1-12.
  7. Yun GW, Lee H. Effect of Cheongawongagam Extract on the Ovariectomized Rat Model of Osteoporosis. Journal of Physiology & Pathology in Korean Medicine. 2020;34(1):14-23. https://doi.org/10.15188/kjopp.2020.02.34.1.14
  8. Kim C, Yang Y, Hwang E. Development of bitter melon juice mixed with condensed oat juice and its hypoglycemic effect in streptozotocin-induced diabetic rats. Korean J Hum Ecol. 2016;25:227-38. https://doi.org/10.5934/kjhe.2016.25.2.227
  9. Son H-K, Han J-H, Lee J-J. Anti-diabetic effect of the mixture of mulberry leaf and green tea powder in rats with streptozotocin-induced diabetes. Korean Journal of Food Preservation. 2014;21(4):549-59. https://doi.org/10.11002/kjfp.2014.21.4.549
  10. Lee HJ, Moon J-H, Lee W-M, Lee SG, Kim A-K, Woo Y-H, et al. Charantin contents and fruit characteristics of bitter gourd (Momordica charantia L.) accessions. Protected Horticulture and Plant Factory. 2012;21(4):379-84. https://doi.org/10.12791/KSBEC.2012.21.4.379
  11. Lee S-J, Shin J-H, Ju J-C, Kang S-K, Sung N-J. Hypoglycemic and hypolipidemic effects of Orostachys japonicus with medicinal herbs in streptozotocin-induced diabetic rats. Journal of the Korean Society of Food Science and Nutrition. 2013;42(4):587-94. https://doi.org/10.3746/jkfn.2013.42.4.587
  12. Roh SG, Choi WC. Antidiabetic synergistic effects of medicinal plant extract mixtures on db/db mice. Journal of Life Science. 2011;21(2):165-75. https://doi.org/10.5352/JLS.2011.21.2.165
  13. Kim K-H, Roh S-G, Li C-R, Jin C-F, Kim A, Choi W-C. Anti-diabetic effects of banaba leaf extracts (Lagerstroemia speciosa Pers.) through solvents. Journal of Life Science. 2008;18(9):1305-11. https://doi.org/10.5352/JLS.2008.18.9.1305
  14. Kim N-S, Choi B-K, Lee S-H, Jang H-H, Kim J-B, Kim H-R, et al. Effects of Allium hookeri on glucose metabolism in type II diabetic mice. Korean Journal of Pharmacognosy. 2015;46(1):78-83.
  15. Kim M-W. Effect of bitter melon on plasma blood glucose and cholesterol levels in streptozotocin induced diabetic rats. Journal of the East Asian Society of Dietary Life. 2013;23(6):704-12.
  16. Kim HK, Kim MJ, Cho HY, Kim EK, Shin DH. Antioxidative and anti-diabetic effects of amaranth (Amaranthus esculantus) in streptozotocin-induced diabetic rats. Cell Biochemistry and Function: Cellular biochemistry and its modulation by active agents or disease. 2006;24(3):195-9. https://doi.org/10.1002/cbf.1210
  17. Myoung K-S, Ahn Y-T, Lee M-H, Park D-Y, Ahn Y-M, Huh C-S. Fingerroot (Boesenbergia pandurata) extract inhibits the accumulation of visceral fat in C57BL/6J mice. Journal of the Korean Society of Food Science and Nutrition. 2013;42(1):26-32. https://doi.org/10.3746/jkfn.2013.42.1.026
  18. Diez JJ, Iglesias P. The role of the novel adipocyte-derived hormone adiponectin in human disease. Eur J Endocrinol. 2003;148(3):293-300. https://doi.org/10.1530/eje.0.1480293
  19. Kershaw EE, Flier JS. Adipose tissue as an endocrine organ. The Journal of Clinical Endocrinology & Metabolism. 2004;89(6):2548-56. https://doi.org/10.1210/jc.2004-0395
  20. Cho HK. Diabetes Mellitus and Disorder of Lipid Metabolism. Journal of Korean Endocrine Society. 2006;21(2):101-5. https://doi.org/10.3803/jkes.2006.21.2.101
  21. Kim DJ, Song KE, Park J-W, Cho HK, Lee K-W, Huh KB. Clinical characteristics of Korean type 2 diabetic patients in 2005. Diabetes research and clinical practice. 2007;77(3):S252-S7. https://doi.org/10.1016/j.diabres.2007.01.067
  22. Youn B-S, Yu K-Y, Park HJ, Lee NS, Min SS, Youn MY, et al. Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus. The Journal of Clinical Endocrinology & Metabolism. 2004;89(1):150-6. https://doi.org/10.1210/jc.2003-031121
  23. Steppan CM, Bailey ST, Bhat S, Brown EJ, Banerjee RR, Wright CM, et al. The hormone resistin links obesity to diabetes. Nature. 2001;409(6818):307-12. https://doi.org/10.1038/35053000
  24. Bado A, Levasseur S, Attoub S, Kermorgant S, Laigneau J-P, Bortoluzzi M-N, et al. The stomach is a source of leptin. Nature. 1998;394(6695):790-3. https://doi.org/10.1038/29547
  25. Maffei a, Halaas J, Ravussin E, Pratley R, Lee G, Zhang Y, et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature medicine. 1995;1(11):1155-61. https://doi.org/10.1038/nm1195-1155
  26. Silha JV, Krsek M, Skrha JV, Sucharda P, Nyomba B, Murphy LJ. Plasma resistin, adiponectin and leptin levels in lean and obese subjects: correlations with insulin resistance. European journal of endocrinology. 2003;149(4):331-6. https://doi.org/10.1530/eje.0.1490331
  27. Cnop M, Landchild MJ, Vidal J, Havel PJ, Knowles NG, Carr DR, et al. The concurrent accumulation of intra-abdominal and subcutaneous fat explains the association between insulin resistance and plasma leptin concentrations: distinct metabolic effects of two fat compartments. Diabetes. 2002;51(4):1005-15. https://doi.org/10.2337/diabetes.51.4.1005
  28. Aizawa K, Inakuma T. Dietary capsanthin, the main carotenoid in paprika (Capsicum annuum), alters plasma high-density lipoprotein-cholesterol levels and hepatic gene expression in rats. British Journal of Nutrition. 2009;102(12):1760-6. https://doi.org/10.1017/S0007114509991309
  29. Vaidya H, Prajapati A, Rajani M, Sudarsanam V, Padh H, Goyal RK. Beneficial Effects of Swertiamarin on Dyslipidaemia in Streptozotocin-induced Type 2 Diabetic Rats. Phytotherapy Research. 2012;26(8):1259-61. https://doi.org/10.1002/ptr.3708