Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Inhibitory Effect of the Rodgersia podophylla Leave Extracts against Cell Proliferation through Activation of NF-κB in Human Colorectal Cancer Cells

도깨비부채 잎 추출물의 NF-κB 활성화를 통한 대장암 세포 HCT116에 대한 세포생육 억제활성

  • Kim, Jeong Dong (Department of Medicinal Plant Resources, Andong National University) ;
  • Jeong, Jin Boo (Department of Medicinal Plant Resources, Andong National University)
  • 김정동 (국립안동대학교 생약자원학과, 대학원) ;
  • 정진부 (국립안동대학교 생약자원학과)
  • Received : 2020.05.12
  • Accepted : 2020.09.23
  • Published : 2020.10.01
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Abstract

In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of leaves from Rodgersia podophylla against human colorectal cancer cells, HCT116. RPL dose-dependently decreased the cell viability through RPL-induced apoptosis in HCT116 cells. RPL induced inactivation the nuclear factor κB(NF-κB) through blocking IκB-α degradtion and P65 nuclear accumulation. The inhibition of GSK3β by LiCl attenuated RP-L-mediated NF-κB signaling inactivation. In addition, RP-L induced GSK3β activation. Based on these findings, RPL may be a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer.

본 연구 결과들을 바탕으로 도깨비부채 잎(RPL)은 GSK3β활성화를 통해 IκB-α를 인산화시켜 단백질 분해를 유도하고 IκB-α분해로 인해 p65 핵내 전이를 유도하여 NF-κB 신호전달을 활성화시킨다. 이러한 NF-κB 신호전달 활성화를 통해 대장암의 세포생육을 억제하는 것으로 추정된다. 본 결과는 도깨비부채 잎을 소재로 항암을 목적으로 한 천연치료제 및 대체보완소재 개발에 활용할 수 있다고 판단된다. 그러나 도깨비부채 잎의 대장암에 대한 세포생육 억제와 작용기전의 정확한 관련성과 세포생육 억제활성 물질 분석을 위해 추가적인 연구가 필요할 것으로 사료된다.

Keywords

References

  1. Arnold, M., M.S. Sierra, M. Laversanne, I. Soerjomataram, A. Jemal and F. Bray. 2017. Global patterns and trends in colorectal cancer incidence and mortality. Gut 66:683-691. https://doi.org/10.1136/gutjnl-2015-310912
  2. Chin, Y.W., E.Y. Park, S.Y. Seo, K.D. Yoon, M.L. Ahn, Y.G. Suh, S.G. Kim and J. Kim. 2006. A novel iNOS and COX-2 inhibitor from the aerial parts of Rodgersia podophylla. Bioorg. Med. Chem. Lett. 16:4600-4602. https://doi.org/10.1016/j.bmcl.2006.06.009
  3. Dolcet, X., D. Llobet, J. Pallares and X. Matias-Guiu. 2005. NF-${\kappa}B$ in development and progression of human cancer. Virchows Arch. 446:475-482. https://doi.org/10.1007/s00428-005-1264-9
  4. Jeong, J.B., X. Yang, R. Clark, J.E. Choi, S.J Baek and S.H. Lee. 2013. A mechanistic study of the proapoptotic effect of tolfenamic acid: Involvement of NF-${\kappa}B$ activation. Carcinogenesis 34:2350-2360. https://doi.org/10.1093/carcin/bgt224
  5. Kim, H.N., J.D. Kim, H.J Son, G.H. Park, H.J. Eo and J.B. Jeong. 2019. Anti-cancer activity of the leave extracts of Rodgersia podophylla through ${\beta}$-catenin proteasomal degradation in human cancer cells. Korean J. Plant Res. 32(5):442-447.
  6. Kim, H.N., J.D. Kim, S.B. Park, H.J Son, G.H. Park, H.J. Eo, H.S. Kim and J.B. Jeong. 2020. Anti-inflammatory activity of the extracts from Rodgersia podophylla leaves through activation of Nrf2/HO-1 pathway, and inhibition of NF-${\kappa}B$ and MAPKs pathway in mouse macrophage cells. Inflamm. Res. 69:233-244. https://doi.org/10.1007/s00011-019-01311-2
  7. Meyerhardt, J.A. and R.J. Mayer. 2005. Systemic therapy for colorectal cancer. N. Engl. J. Med. 352:476-87. https://doi.org/10.1056/NEJMra040958
  8. Park, B.K., J.H. Lee, C.K. Cho, H.K. Shin, S.K. Eom and H.S. Yoo. 2008. Systemic review of clinical studies about oriental medical treatment of cancer in Korea. Korean J. Orient. Int. Med. 29(4):1061-1074.
  9. Wang, H., T.O. Khor, L. Shu, Z. Su, F. Fuentes, J.H. Lee and A.N.T. Kong. 2012. Plants against cancer: A review on natural phytochemicals in preventing and treating cancers and their druggability. Anti-Cancer Agents Med. Chem. 12: 1281-1305. https://doi.org/10.2174/187152012803833026
  10. Wyllie, A.H., G.J. Beattie and A.D. Hargreaves. 1981. Chromatin changes in apoptosis. Histochem. J. 13:681-692. https://doi.org/10.1007/BF01002719
  11. Zhang, S., C.N. Ong, H.M. Shen. 2004. Critical roles of intracellular thiols and calcium in parthenolide-induced apoptosis in human colorectal cancer cells. Cancer Lett. 208:143-153. https://doi.org/10.1016/j.canlet.2003.11.028