The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Comparison of an Immunochromatographic Assay Kit with DAS-ELISA for Large-Scale Diagnosis and Molecular Discrimination of Satsuma Dwarf Virus Collected from Citrus Orchards

  • Kato, Mitsuhiro (Shizuoka Prefectural Research Institute of Agriculture and Forestry, Fruit Tree Research Center) ;
  • Tomimura, Kenta (Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO)) ;
  • Ishii, Kanako (Shizuoka Prefectural Research Institute of Agriculture and Forestry, Fruit Tree Research Center)
  • Received : 2020.06.04
  • Accepted : 2020.08.16
  • Published : 2020.10.01
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Abstract

Satsuma dwarf virus (SDV) seriously damages citrus production by reducing the quality and yield of fruit. To avoid contamination with SDV, mother trees are checked to be SDV-free in advance of nursery tree distribution. In this study, we compared an immunochromatographic assay (ICA) kit with double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for large-scale diagnosis of SDV in orchardgrown trees in Shizuoka Prefecture, Japan. The two methods gave conflicting results for 11 of 1,705 samples, all of which were negative by DAS-ELISA but positive by ICA. The samples scored as positive by either DAS-ELISA or ICA were analyzed by reverse transcription polymerase chain reaction and all were confirmed to be positive. These results validate the use of ICA as a screening method for large-scale diagnosis. Strain discrimination revealed that 16 of 22 isolates belonged to SDV, while citrus mosaic virus (CiMV) infection only and co-infection (SDV and CiMV) were in a minority.

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References

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