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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Improved human hematopoietic reconstitution in HepaRG co-transplanted humanized NSG mice

  • Kim, Jin (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Ryu, Bokyeong (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Ukjin (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Chang-Hwan (The 4th R&D Institute-6, Agency for Defense Development) ;
  • Hur, Gyeung-Haeng (The 4th R&D Institute-6, Agency for Defense Development) ;
  • Kim, C-Yoon (Stem Cell Biology, School of Medicine, Konkuk University) ;
  • Park, Jae-Hak (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University)
  • Received : 2019.12.14
  • Accepted : 2020.03.17
  • Published : 2020.09.30
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Abstract

Several humanized mouse models are being used to study humanspecific immune responses and diseases. However, the pivotal needs of fetal tissues for the humanized mice model have been huddled because of the demand for ethical and medical approval. Thus, we have verified the hematopoietic and immunomodulatory function of HepaRG and developed a new and easy humanized mouse model to replace the use of fetal liver tissue. HepaRG co-transplanted Hu-NSG mice significantly increased CD45+ lymphocytes and CD19+ B cells and CD3+ T cells than normal Hu-NSG, suggesting enhanced reconstitution of the human immune system. These results have improved the applicability of humanized mice by developing new models easily accessible.

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References

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