Applied Microscopy

  • Volume 50
  • /
  • Pages.26.1-26.3
  • /
  • 2020
  • /
  • 2287-5123(pISSN)
  • /
  • 2287-4445(eISSN)
Korean Society of Microscopy (한국현미경학회)
권호 표지
DOI QR코드

DOI QR Code

Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images

  • Jihun Kang (School of Integrated Technology, Yonsei University) ;
  • Eun-Hye Kang (Department of Plastic and Reconstruction Surgery, College of Medicine, Yonsei University) ;
  • Young-Shik Yun (School of Integrated Technology, Yonsei University) ;
  • Seungmuk Ji (Yonsei Institute of Convergence Technology, Yonsei University) ;
  • In-Sik Yun (Department of Plastic and Reconstruction Surgery, College of Medicine, Yonsei University) ;
  • Jong-Souk Yeo (School of Integrated Technology, Yonsei University)
  • Received : 2020.10.15
  • Accepted : 2020.11.12
  • Published : 2020.12.31
⟨ Previous Next ⟩

Abstract

The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500- nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.

Keywords

Acknowledgement

This research was supported by Research Program through the National Institute of Ecology (NIE-Basic Research-2020-18) and also supported by National Research Foundation (NRF) grant funded by the Ministry of Education, under "Basic Science Research Program" (NRF-2017R1D1A1B04033182).

References

  1. M.M. Stevens, J.H. George, Exploring and engineering the cell surface interface. Science. 310, 1135 (2005)
  2. J.L. Tan, J. Tien, D.M. Pirone, D.S. Gray, K. Bhadriraju, C.S. Chen, Cells lying on a bed of microneedles: an approach to isolate mechanical force. Proc. Natl. Acad. Sci. 100, 1484 (2003)
  3. L. Trichet, J. Le Digabel, R.J. Hawkins, S.R.K. Vedula, M. Gupta, C. Ribrault, P. Hersen, R. Voituriez, B. Ladoux, Evidence of a large-scale mechanosensing mechanism for cellular adaptation to substrate stiffness. Proc. Natl. Acad. Sci. 109, 6933 (2012)
  4. Y.S. Yun, E.H. Kang, S. Ji, S.B. Lee, Y.O. Kim, I.S. Yun, J.S. Yeo, Quantitative correlation of nanotopography with cell spreading via focal adhesions using adipose-derived stem cells. Adv. Biosyst. 4, 2000092 (2020)