대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)

  • 제34권3호
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  • Pages.67-75
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  • 2020
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  • 1226-8038(pISSN)
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  • 2287-9463(eISSN)
대한한방소아과학회 (The Association of Korean Oriental Pediatrics)
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황련감초 추출물의 상피지방장벽 생성을 통한 Th2 분화 조절

Effect of Th2 Differentiation Control through Formation of Skin Fat Barrier on Coptidis Rhizoma & Glycyrrhiza Uralensis extract

  • 박범찬 (부산대학교한방병원 한방소아과) ;
  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 서일복 (세명대학교 한의과대학 해부학교실) ;
  • 천진홍 (부산대학교한방병원 한방소아과) ;
  • 김기봉 (부산대학교한방병원 한방소아과)
  • Park, Beom Chan (Department of Korean Pediatrics, Pusan National University Korean Medicine Hospital) ;
  • Ahn, Sang Hyun (Department of Anatomy, College of Korean Medicine, Semyung University) ;
  • Seo, Il Bok (Department of Anatomy, College of Korean Medicine, Semyung University) ;
  • Cheon, Jin Hong (Department of Korean Pediatrics, Pusan National University Korean Medicine Hospital) ;
  • Kim, Ki Bong (Department of Korean Pediatrics, Pusan National University Korean Medicine Hospital)
  • 투고 : 2020.07.24
  • 심사 : 2020.08.22
  • 발행 : 2020.08.31
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초록

Objectives This study is conducted to evaluate Th2 skewed condition control through skin fat barrier formation from the treatment using Coptidis Rhizoma and Glycyrrhiza uralensis extract. Methods The 6-week-old NC/Nga mice were divided into 4 groups: Control group (Ctrl), lipid barrier eliminate treatment group (LBET), Coptidis Rhizoma and Glycyrrhiza uralensis feeding treatment after lipid barrier elimination group (CGFT), dexamethasone feeding treatment after lipid barrier elimination group (DxFT). After 3 days, differences in skin condition, improvement of skin fat barrier, and control of Th2 skewed condition of each group were observed. Results Pathologic skin damage and tissue changes were less in the CGFT group than in the LBET and DxFT groups, and Transepidermal water loss (TEWL) and pH were also significantly decreased (p < 0.05). The filaggrin intensity and positive response also increased significantly in the CGFT group (p < 0.05). Kallikrein-related peptidase (KLK) 7, Protease activated receptor (PAR)-2, Thymic stromal lymphopoietin (TSLP), Interleukin (IL)-4, and the products of the Th2 differentiation process also showed a significant decrease compared to the LBET and DxFT groups (all p < 0.05). Conclusions The Coptidis Rhizoma and Glycyrrhiza uralensis extract causes skin barrier recovery and function recovery through the formation of skin fat barrier. This leads to the conclusion that Coptidis Rhizoma and Glycyrrhiza uralensis extract can control Th2 differentiation through the formation of skin fat barrier.

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참고문헌

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