한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제24권2호
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  • Pages.135-147
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  • 2020
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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PVDF Nanofiber Scaffold Coated with a Vitronectin Peptide Facilitates the Neural Differentiation of Human Embryonic Stem Cells

  • Jeon, Byeong-Min (Dept. of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Yeon, Gyu-Bum (Dept. of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Goo, Hui-Gwan (AMO Lifescience Co., Ltd.) ;
  • Lee, Kyung Eun (Advance Analysis Center, Korean Institute of Science and Technology) ;
  • Kim, Dae-Sung (Dept. of Biotechnology, College of Life Sciences and Biotechnology, Korea University)
  • 투고 : 2020.04.20
  • 심사 : 2020.05.16
  • 발행 : 2020.06.30
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초록

Polyvinylidene fluoride (PVDF) is a stable and biocompatible material that has been broadly used in biomedical applications. Due to its piezoelectric property, the electrospun nanofiber of PVDF has been used to culture electroactive cells, such as osteocytes and cardiomyocytes. Here, taking advantage of the piezoelectric property of PVDF, we have fabricated a PVDF nanofiber scaffolds using an electrospinning technique for differentiating human embryonic stem cells (hESCs) into neural precursors (NPs). Surface coating with a peptide derived from vitronectin enables hESCs to firmly adhere onto the nanofiber scaffolds and differentiate into NPs under dual-SMAD inhibition. Our nanofiber scaffolds supported the differentiation of hESCs into SOX1-positive NPs more significantly than Matrigel. The NPs generated on the nanofiber scaffolds could give rise to neurons, astrocytes, and oligodendrocyte precursors. Furthermore, comparative transcriptome analysis revealed the variable expressions of 27 genes in the nanofiber scaffold groups, several of which are highly related to the biological processes required for neural differentiation. These results suggest that a PVDF nanofiber scaffold coated with a vitronectin peptide can serve as a highly efficient and defined culture platform for the neural differentiation of hESCs.

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참고문헌

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