한국유기농업학회지 (Korean Journal of Organic Agriculture)

  • 제28권4호
  • /
  • Pages.659-677
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  • 2020
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  • 1229-3571(pISSN)
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  • 2287-819X(eISSN)
한국유기농업학회 (Korean Association of Organic Agriculture)
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익모초 에탄올 추출물의 항산화 및 항염증 활성

Anti-oxidant and Anti-inflammatory Effects of Ethanol Extracts from Leonurus japonicus Houtt. on LPS-induced RAW 264.7 Cells

  • 최유나 (전북대학교 농업생명과학대학 농학과) ;
  • 최유경 (전북대학교 농업생명과학대학 작물생명과학과) ;
  • 난리 (전북대학교 농업생명과학대학 작물생명과학과) ;
  • 추병길 (전북대학교 농업생명과학대학 작물생명과학과)
  • 투고 : 2020.10.26
  • 심사 : 2020.11.04
  • 발행 : 2020.11.30
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초록

본 실험에서는 익모초 지상부 에탄올 추출물의 항산화 및 항염증 효과를 확인하여 건강 기능성 소재로서의 가능성을 평가하고자 하였다. DPPH 및 ABTS radical 소거 활성, 환원력, 총 폴리페놀 및 플라보노이드 함량을 통해 익모초의 항산화능을 측정한 결과, 400 ㎍/mL, 1500 ㎍/mL의 농도에서 57.8%, 62.3%의 DPPH 및 ABTS radical 소거활성을 보였고 환원력 또한 농도 의존적으로 증가하는 경향을 보였다. 총 폴리페놀 함량 및 플라보노이드 함량은 1 mg/mL의 농도에서 각각 51.40 ± 0.47 mg of gallic acid equivalents/g, 73.28 ± 0.10 mg of rutin equivalents/g로 나타났으며, 익모초 추출물은 세포 내 ROS의 생성 억제에 있어서 유의적인 효과를 보였다. 익모초의 항염증 효과를 측정한 결과, LPS로 자극해 활성화된 RAW 264.7 cell에서 익모초 추출물(0~400 ㎍/mL)의 세포 독성은 없었으며 LPS 처리로 유도된 세포의 형태학적 변화도 농도의존적으로 완화되는 경향을 보였다. NO 발생량은 LPS 처리군과 비교해 익모초 추출물 처리 시 농도 의존적으로 감소하였고, 400 ㎍/mL에서는 90.3%로 NO의 생성이 억제되었다. 염증성 cytokine (TNF-α, IL1-β)의 생성도 유의적으로 감소하였고 NO를 생성하는 염증성 단백질 iNOS의 발현 또한 억제되었으며 이와 같은 염증성 단백질의 전사를 조절하는 NF-κB (NF-κB, IκB-α) 및 MAPK (ERK, p38)의 인산화 및 활성화 또한 익모초 추출물 처리로 인해 억제됨을 확인하였다. 따라서 익모초 추출물은 NF-κB signaling pathway 및 ERK/p38 MAPK cascade pathway의 조절을 통해 염증성 단백질 및 염증인자의 발현을 감소시킨다고 볼 수 있다. 이러한 결과를 토대로 익모초 지상부 에탄올 추출물은 천연 기능성 소재로서 활용될 가능성이 있으며, 본 연구 결과는 익모초의 고부가가치 향상을 위한 기초자료로 도움이 될 것으로 생각된다.

Leonurus japonicus (L. japonicus) Houtt., a biennial plant in the Lamiaceae family is broadly distributed in Asia such as Korea, China, Japan. The aerial part of L. japonicus is used as a traditional medicine to treat uterine disease including dysmenorrhea, amenorrhea, sterility. In this study, we examined the antioxidant and anti-inflammatory effects of L. japonicus ethanol extracts. The antioxidant activity of L. japonicus was measured by total polyphenol and flavonoid content, and DPPH, ABTS scavenging, reducing power activity, and intracellular ROS expression assay. The anti-inflammatory effects were measured by nitric oxide (NO), cytokines (TNF-α and IL-1β) production and inflammatory protein expression in LPS-induced RAW 264.7 cells. Total polyphenol and flavonoid content of L. japonicus were 51.40 ± 0.47 mg of gallic acid equivalents/g and 73.28 ± 0.10 mg of rutin equivalents/g respectively. DPPH, ABTS radical scavenging activity and reducing power activity tended to increase concentration-dependent and treatment L. japonicus with 400 ㎍/mL reduced ROS production by 69.5%. Furthermore, L. japonicus inhibited NO, TNF-α and IL-1β production in a concentration-dependant manner and reduced the expression levels of inflammatory proteins via regulating NF-κB, MAPK pathway. Therefore, we suggest that L. japonicus could be a natural antioxidants and medicinal source to treat oxidative stress and inflammation-related disease.

키워드

과제정보

본 논문은 농촌진흥청 연구사업(세부과제번호: PJ0142272020)의 지원에 의해 이루어진 것임.

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