Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Discordance between Morphological and Molecular Variations of the Genus Macroramphosus (Macroramphosidae) from Korea

한국산 대주둥치속(대주둥치과) 어류의 형태와 분자 변이의 불일치

  • Sohn, Min-Soo (Department of Marine Biology, Pukyong National University) ;
  • Kim, Jin-Koo (Department of Marine Biology, Pukyong National University)
  • 손민수 (부경대학교 자원생물학과) ;
  • 김진구 (부경대학교 자원생물학과)
  • Received : 2020.04.06
  • Accepted : 2020.11.04
  • Published : 2020.12.31
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Abstract

In order to clarify the taxonomic status of the Korean Macroramphosus species, which were previously confused, we investigated morphological and molecular variations of Macroramphosus (18 individuals) from Korea, and Macroramphosus (35 individuals) from Japan and Taiwan, and compared with those of M. scolopax from type locality (Mediterranean Sea). Although the Korean and Japanese specimens of Macroramphosus were clearly divided into two types in the first dorsal spine length (22.8~32.1% in A-type vs. 15.6~21.4% in B-type), distance between the first dorsal fin and second dorsal fin (6.4~9.7% vs. 8.6~13.3%), and body depth (20.0~28.0% vs. 17.3~22.6%), no genetic differences among all individuals of longspine snipefish between them were found at the specific level [d=0.0~3.3% in control region (CR); 0.0~1.3% in cytochrome b (cytb); 0.0~0.5% in cytochrome c oxidase subunit I (COI)]. Whereas, they were well distinguished in genetics (9.9~11.5% in CR; 3.8~4.6% in cytb; 1.2~3.6% in COI) from those of M. scolopax in Mediterranean Sea. It needs the scientific name of the longspine snipefish (M. scolopax) in Korea be changed as M. japonicus (and/or M. sagifue). However, our results could not find evidence of consistency between morphological and mitochondrial DNA variations which suggests that their differentiation event may occur fairly recently. Further studies using more sensitive markers such as microsatellite are needed to clarify the degree of gene flow between the two types.

본 연구는 예전부터 혼란스러웠던 한국산 대주둥치속, Macroramphosus 어류의 분류학적 위치를 명확히 하기 위해, 한국산 18개체를 일본/대만산 35개체 및 지중해산 M. scolopax와 형태 및 분자 변이를 비교 분석하였다. 한국, 일본 및 대만산 대주둥치속 어류는 제1등지느러미 극조 길이(A-type은 22.8~32.1%, B-type은 15.6~21.4%), 제1등지느러미와 제2등지느러미 사이 길이(A-type은 6.4~9.7%, B-type은 8.6~13.3%), 체고(A-type은 20.0~28.0%, B-type은 17.3~22.6%)에서 두 type으로 명확히 구분되었으나, 유전적으로는 구분되지 않았다(CR에서 0.0~3.3%, cyt b에서 0.0~1.3%, COI에서 0.0~0.5%). 한편, 한국산 대주둥치는 지중해산 M. scolopax와 유전적으로 명확히 구분되어(CR에서 9.9~11.5%, cyt b에서 3.8~4.6%, COI에서 1.2~3.6%), 최근 사용하고 있는 학명 M. scolopax를 M. japonicus (및/또는 M. sagifue)로 변경해야 할 것이다. 그러나, 본 연구에서 두 type 간 형태변이와 분자변이 간 일치성을 찾지 못했으며, 이는 아마도 그들간에 분화가 상당히 최근에 일어났음을 시사한다. 두 type 간 유전자 교류 정도를 파악하려면 향후 microsatellite와 같은 보다 민감한 마커를 이용한 후속 연구가 필요할 것이다.

Keywords

Acknowledgement

이 논문은 2019학년도 부경대학교 연구[II] 교원 지원사업에 의하여 연구되었습니다. 또한 이 논문의 완성도를 높여 주신 심사위원께 감사드립니다.

References

  1. Bilecenoglu, M. 2006. Status of the genus Macroramphosus (Syngnathiformes: Centriscidae) in the eastern Mediterranean Sea. Zootaxa, 1273: 55-64.
  2. Brown, W.M., M. George and A.C. Wilson. 1979. Rapid evolution of animal mitochondrial DNA. Proc. Natl. Acad. Sci., U.S.A., 76: 1967-1971. https://doi.org/10.1073/pnas.76.4.1967.
  3. Carneiro, M., R. Martins, F. Reiner and I. Bastista. 2019. Ichthyofauna of Portugal: Taxonomic diversity, common and scientific names of marine fishes. Vol. I. I.P.M.A., I.P., Lisbon, Portugal, 376pp.
  4. Cheng, Y.Z., T.J. Xu, X.X. Jin, D. Tang, T. Wei, Y.Y. Sun and R.X. Wang. 2012. Universal primers for amplification of the complete mitochondrial control region in marine fish species. Mol. Biol., 46: 727-730. https://doi.org/10.1134/S0026893312040024.
  5. Chyung, M.K. 1954. Korean fishes. Ministry of Trade, Seoul, Korea, p. 207.
  6. Chyung, M.K. 1961. Illustrated encyclopedia the fauna of Korea (2) fishes. The ministry of Education, Seoul, Korea, pp. 298-299.
  7. Chyung, M.K. 1977. The fishes of Korea. Iljisa, Seoul, Korea, pp. 269-270.
  8. Clarke, T.A. 1984. Diet and morphological variation in snipefishes, presently recognized as Macrorhamphosus scolopax, from southeast Australia: evidence for two sexually dimorphic species. Copeia, 1984: 595-608. https://doi.org/10.2307/1445140.
  9. Ehrich, S. 1976. On the taxonomy, ecology and growth of Macroramphosus scolopax (Linnaeus, 1758) (Pisces, Syngnathiformes) from the subtropical northeast Atlantic. Ber. Dt. Wiss. Kommn. Meeresforsch., 24: 251-266.
  10. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic. Acids. Symp. Ser., 41: 95-98.
  11. Hsu, T.H and K.H. Lin. 2008. Genetic relationship among four subspecies of cherry salmon (Oncorhynchus masou) inferred using AFLP. Mol. Phylogenet. Evol., 48: 776-781.
  12. Kai, Y. and T. Nakabo. 2008. Taxonomic review of the Sebastes inermis species complex (Scorpaeniformes: Scorpaenidae). Ichthyol. Res., 55: 238-259. https://doi.org/10.1007/s10228007-0029-7.
  13. Kai, Y., K. Nakayama and T. Nakabo. 2002. Genetic differences among three colour morphotypes of black rockfish, Sebastes inermis, inferred from mtDNA and AFLP analysis. Mol. Ecol., 11: 2591-2598. https://doi.org/10.1046/j.1365-294X.2002.01628.x.
  14. Kim, I.S., Y. Choi, C.L. Lee, Y.J. Lee, B.J. Kim and J.H. Kim. 2005. Illustrated book of Korean fishes. Kyohak Publishing Co. Ltd., Seoul, Korea, 615pp.
  15. Kim, J.K., J.H. Ryu, H.J. Kwon, H.S. Ji, J.H. Park, S.H. Myoung, Y.S. Song, S.J. Lee, H.J. YU, S.E. Bae, S.H. Jang and W.J. Lee. 2019. Distribution map of sea fishes in Korean Peninsula. Maple design, Busan, Korea, 541pp.
  16. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol., 16: 111-120.
  17. Kochzius, M., C. Seidel, A. Antoniou, S.K. Botla, D. Campo, A. Cariani and G. Hreggvidsson. 2010. Identifying fishes through DNA barcodes and microarrays. PLoS One, 5: e12620. https://doi.org/10.1371/journal.pone.0012620.
  18. Kumar, S., G. Stecher, M. Li, C. Knyaz and K. Tamura. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol., 35: 1547-1549. https://doi.org/10.1093/molbev/msy096.
  19. Kuranaga, I. and K. Sasaki. 2000. Larval development in a snipefish (Macroramphosus scoplopax) from Japan with notes on eastern Pacific and Mediterranean Macroramphosus larvae (Gasterosteiformes, Macroramphosidae). Ichthyol. Res., 47: 101106.
  20. Lindberg, G.U. and M.I. Legeza. 1959. Fishes of the Sea of Japan and the adjacent areas of the Sea of Okhotsk and the Yellow Sea, part 2, Teleostomi, xii. Israel Program for Scientific Translations, Jerusalem.
  21. MABIK (Marine Biodiversity Institute of Korea). 2018. National list of marine species. Namu Press, Seocheon, Korea, p. 33.
  22. Matthiessen, B., H.O. Fock and H. von. Westernhagen. 2003. Evidence for two sympatric species of snipefishes Macroramphosus spp.(Syngnathiformes, Centriscidae) on Great Meteor Seamount. Helgol. Mar. Res., 57: 63. https://doi.org/10.1007/s10152-002-0133-z.
  23. Miyazaki, E., K. Sasaki, T. Mitani, M. Ishida and S. Uehara. 2004. The occurrence of two species of Macroramphosus (Gasterosteiformes: Macroramphosidae) in Japan: morphological and ecological observations on larvae, juveniles, and adults. Ichthyol. Res., 51: 256-262. https://doi.org/10.1007/s10228004-0227-5.
  24. Mohr, E. 1937. Revision der Centriscidae (Acanthopterygii, Centrisciformes). Dana Rep., 13: 1-69.
  25. Muto, N., Y. Kai and T. Nakabo. 2011. Genetic and morphological differences between Sebastes vulpes and S. zonatus(Teleostei: Scorpaeniformes: Scorpaenidae). Fish. Bull., 109: 429-439.
  26. Muto, N., Y. Kai and T. Nakabo. 2019. Taxonomic review of the Sebastes vulpes complex (Scorpaenoidei: Sebastidae). Ichthyol. Res., 66: 9-29. https://doi.org/10.1007/s10228-0180641-8.
  27. Nelson, J.S., T.C. Grande and M.V.H. Wilson. 2016. Fishes of the world, 5th ed. John Wiley & Sons Inc., New Jersey, U.S.A., 410pp.
  28. NIBR (National Institute of Biological Resources). 2011. National list of species of Korea (Vertebrates). NIBR, Incheon, Korea, p. 69.
  29. Noguchi, T., K. Sakuma, T. Kitahashi, H. Itoh, Y. Kano, G. Shinohara and S. Kojima. 2014. No genetic deviation between two morphotypes of the snipefishes (Macroramphosidae: Macroramphosus) in Japanese waters. Ichthyol. Res., 62: 368-373. https://doi.org/10.1007/s10228-014-0443-6.
  30. Noguchi, T., T. Kitahashi, G. Shinohara, J. Hashimoto and S. Kojima. 2016. Morphological differences among snipefishes (Macroramphosidae: Macroramphosus) from the Pacific and Atlantic oceans. Plankton Benthos Res., 11: 147-150. https://doi.org/10.3800/pbr.11.147.
  31. Okada, Y. and K. Suzuki. 1951. A review on the Macrorhamphosus fishes of Japan. Rep. Fac. Fish. Pref. Univ. Mie, 1: 7-11.
  32. Robalo, J.I., C. Sousa-Santos, H. Cabral, R. Castilho and V.C. Almada. 2009. Genetic evidence fails to discriminate between Macroramphosus gracilis Lowe 1839 and Macroramphosus scolopax (Linnaeus 1758) in Portuguese waters. Mar. Biol., 156: 1733-1737. https://doi.org/10.1007/s00227009-1197-y.
  33. Senou, H. 2013. Macroramphosidae. In: Nakabo, T. (ed.), Fishes of Japan with pictorial keys to the species, 3rd ed. Tokai Univ. Press, Kanagawa, Japan, p. 611.
  34. Shao, K.T., H.C. Ho, P.L. Lin, P.F. Lee, M.Y. Lee, C.Y. Tsai, Y.C. Liao and Y.C. Lin. 2008. A checklist of the fishes of southern Taiwan, northern South China Sea. Raffles. Bull. Zool., 19: 233-271.
  35. Tavare, S. 1986. Some probabilistic and statistical problems in the analysis of DNA sequences. Lectures Math. Life Sci., 17: 57-86.
  36. Tompson, J.D., D.G. Higgins and T.J. Gibson. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence weighthing, position-specific gap penalties, and weight matrix choice. Nucleic Acids Res., 22: 4673-4680.
  37. Wang, J., P. Sun and F. Yin. 2014. Low mtDNA Cytb diversity and shallow population structure of Eleutheronema tetradactylum in the East China Sea and the South China Sea. Biochem. Syst. Ecol., 55: 268-274. https://doi.org/10.1016/j.bse.2014.03.026.
  38. Ward, R.D., T.S. Zemlak, B.H. Innes, P.R. Last and P.D. Hebert. 2005. DNA barcoding Australia's fish species. Philos. Trans. R. Soc. Lond. B. Biol. Sci., 360: 1847-1857. https://doi.org/10.1098/rstb.2005.1716.
  39. Youn, C.H. 2002. Fishes of Korea with pictorial key and systematic list. Academic Seojeok, Seoul, Korea, 694pp.
  40. Zheng, W., L. Zou and Z. Han. 2015. Genetic analysis of the populations of Japanese anchovy Engraulis japonicus from the Yellow Sea and East China Sea based on mitochondrial cytochrome b sequence. Biochem. Syst. Ecol., 58: 169-177. https://doi.org/10.1016/j.bse.2014.12.007.