The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of Persicae Semen for Atopic Dermatitis Skin Tissue and Regulate to Inflammation Mediator in Serum

도인(桃仁)의 아토피 피부염 모델 피부조직 및 혈청 내 염증매개물질 조절 효과

  • Kim, Sangwoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Hong, SooYeon (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Kwon, Boguen (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Myunghyun (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Sang-bae (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Jin, Dae-hwan (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Choi, Woochan (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Sohn, Youngjoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Jung, Hyuk-Sang (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
  • 김상우 (경희대학교 한의과대학 해부학교실) ;
  • 홍수연 (경희대학교 한의과대학 해부학교실) ;
  • 권보근 (경희대학교 한의과대학 해부학교실) ;
  • 김명현 (경희대학교 한의과대학 해부학교실) ;
  • 김상배 (경희대학교 한의과대학 해부학교실) ;
  • 진대환 (경희대학교 한의과대학 해부학교실) ;
  • 최우찬 (경희대학교 한의과대학 해부학교실) ;
  • 손영주 (경희대학교 한의과대학 해부학교실) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실)
  • Received : 2020.06.03
  • Accepted : 2020.07.25
  • Published : 2020.07.30
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Abstract

Objective : The objective of this study was to demonstrate the effect of Persicae Semen (PS) in DNCB-induced atopic dermatitis mouse and HaCaT cell. Methods : The BALB/c mice were divided into four groups. To develop atopic dermatitis, 200 ㎕ of 1 and 0.5% DNCB solution was put on the back of mice in the Control group, the PS-Low group and the PS-High group once a day. After application of DNCB, 200 ㎕ of the PS extract was also treated. The Normal group was given PBS. The mice dorsal skin was stained with Masson's trichrome, H&E, and toluidine blue to evaluate the thickness of the epidermis and dermis, infiltration of eosinophils and mast cells respectively. ELISA was applied to measure the serum level of IgE and IL-6. Toxicity of PS was measured by MTS assay in HaCaT cell. To investigate the effects of PS on HaCaT cells, cells were pre-treated with PS for 1h, and then stimulated with TNF-α and IFN-γ. After 24 hours, the expression of TARC was analyzed using RT-PCR. Results : PS not only significantly diminished the thickness of the epidermis and dermis, but also reduced the infiltration of eosinophil and mast cell in skin lesion. PS also reduced the serum IgE and IL-6 level which plated important roles in the atopic dermatitis. The expression of TARC was decreased significantly in TNF-α/IFN-γ stimulated HaCaT cell. Conclusion : These results suggest that PS may be effective in alleviating the atopic dermatitis induced by DNCB and inflammation by TNF-α/IFN-γ.

Keywords

References

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