환경생물 (Korean Journal of Environmental Biology)

  • 제38권1호
  • /
  • Pages.40-46
  • /
  • 2020
  • /
  • 1226-9999(pISSN)
  • /
  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
권호 표지
DOI QR코드

DOI QR Code

암모니아 급성노출에 따른 대왕범바리(Epinephelus fuscoguttatus ♀×E. lanceolatus ♂)의 혈액성상 및 혈장성분의 변화

Changes of hematological parameters and plasma components in the hybrid grouper(Epinephelus fuscoguttatus ♀×E. lanceolatus ♂) by acute ammonia exposure

  • 김광민 (선문대학교 수산생명의학과) ;
  • 이희진 (선문대학교 수산생명의학과) ;
  • 윤한빈 (선문대학교 수산생명의학과) ;
  • 조재황 (서해수산연구소 양식산업과) ;
  • 김석렬 (서해수산연구소 양식산업과) ;
  • 이경미 (서해수산연구소 양식산업과) ;
  • 김준환 (서해수산연구소 양식산업과)
  • Kim, Kwang-Min (Department of Aquatic Life and Medical Science, Sun Moon University) ;
  • Lee, Hee-Jin (Department of Aquatic Life and Medical Science, Sun Moon University) ;
  • Yun, Han Bin (Department of Aquatic Life and Medical Science, Sun Moon University) ;
  • Cho, Jae-Hwang (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Seok-Ryel (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Lee, Kyung Mi (West Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kim, Jun-Hwan (West Sea Fisheries Research Institute, National Institute of Fisheries Science)
  • 투고 : 2019.12.23
  • 심사 : 2020.02.10
  • 발행 : 2020.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 실험에서 암모니아 급성 노출은 대왕범바리의 혈액학적 성상 및 혈장 성분에 유의적 변화를 나타내었다. 본 실험의 결과 20mg L-1 이상의 급성 암모니아 노출은 대왕범바리의 혈액학적 성상 및 혈장 성분에 영향을 주어 생리적 변화를 일으키며, 40mg L-1의 급성 암모니아 농도는 대량 폐사를 유발할 수 있음을 나타낸다. 향후 대왕범바리 바이오플락 양식기술 적용을 위한 양식연구에 이러한 결과를 지표로 활용할 수 있을 것이다.

Hybrid grouper(Epinephelus fuscoguttatus ♀×E. lanceolatus ♂) (mean weight 25.7±3.5g, mean length 11.2±0.9cm) were exposed to different ammonia concentrations of 0, 5, 10, 20, and 40mg L-1 for 96 hours. The hematological hematocrit and hemoglobin parameters of the hybrid grouper were significantly decreased by 20 mg L-1 ammonia exposure. In the organic plasma components, calcium was significantly decreased, whereas there was no change in magnesium. In the organic plasma components, the glucose and cholesterol values of the hybrid grouper were significantly increased by ammonia exposure. In the enzymatic plasma components, the ALP(Alkaline phosphatase) value of the hybrid grouper was also significantly increased by ammonia exposure. The results of this study demonstrate that acute ammonia exposure to hybrid grouper induced changes in hematological parameters and plasma components. Therefore, acute ammonia exposure over 20 mg L-1 appears to be toxic to hybrid grouper and the results can be used as a major indicator in breeding hybrid grouper.

키워드

참고문헌

  1. Bijvelds M, JA Velden, ZI Kolar and G Flik. 1998. Magnesium transport in freshwater teleosts. J. Exp. Biol. 201:1981-1990. https://doi.org/10.1242/jeb.201.13.1981
  2. Choi YS and SY Lee. 1992. Serum cholesterol and 3-hydroxy-3-methylglutaryl coenzyme A reductase. J. Korean Soc. Food Sci. Nutr. 21:580-593.
  3. Das PC, S Ayyappan, JK Jena and BK Das. 2004. Acute toxicity of ammonia and its sub-lethal effects on selected haematological and enzymatic parameters of mrigal, Cirrhinus mrigala (Hamilton). Aquac. Res. 35:134-143. https://doi.org/10.1111/j.1365-2109.2004.00994.x
  4. Evans JJ, DJ Pasnik, GC Brill and PH Klesius. 2006. Un-ionized ammonia exposure in Nile tilapia: toxicity, stress response, and susceptibility to Streptococcus agalactiae. N. Am. J. Aquacult. 68:23-33. https://doi.org/10.1577/A05-032.1
  5. Groff JM and JG Zinkl. 1999. Hematology and clinical chemistry of cyprinid fish: common carp and goldfish. Vet. Clin. N. Am.-Exot. Anim. Pract. 2:741-776. https://doi.org/10.1016/S1094-9194(17)30120-2
  6. Hrubec TC, SA Smith and JL Robertson. 1996. Nitrate toxicity: a potential problem of recirculating systems. In: Aquacultural Engineering Society Proceedings II: Successes and Failures in Commercial Recirculating Aquaculture. Northeast Regional Agricultural Engineering Service Cooperative Extension (NRAES). Ithaca, NY.
  7. Ip YK and SF Chew. 2010. Ammonia production, excretion, toxicity, and defense in fish: a review. Front. Physiol. 1:134. https://doi.org/10.3389/fphys.2010.00134
  8. Kim JH and JC Kang. 2014. The selenium accumulation and its effect on growth, and haematological parameters in red sea bream, Pagrus major, exposed to waterborne selenium. Ecotox. Environ. Safe. 104:96-102. https://doi.org/10.1016/j.ecoenv.2014.02.010
  9. Kim JH and JC Kang. 2016. The chromium accumulation and its physiological effects in juvenile rockfish, Sebastes schlegelii, exposed to different levels of dietary chromium (Cr6$^+$) concentrations. Environ. Toxicol. Pharmacol. 41:152-158. https://doi.org/10.1016/j.etap.2015.12.001
  10. Kim JH, KW Kim, SH Bae, SK Kim, SK Kim and JH Kim. 2017a. Alterations in hematological parameters and antioxidant responses in the biofloc-reared flatfish Paralichthys olivaceus following ammonia exposure. Korean J. Fish. Aquat. Sci. 50:750-755. https://doi.org/10.5657/KFAS.2017.0750
  11. Kim JH, HJ Park, IK Hwang, JM Han, DH Kim, CW Oh and JC Kang. 2017b. Alterations of growth performance, hematological parameters, and plasma constituents in the sablefish, Anoplopoma fimbria depending on ammonia concentrations. Fish. Aquat. Sci. 20:4. https://doi.org/10.1186/s41240-017-0049-9
  12. Kim JH, EH Jeong, SR Kim, SK Kim, SK Kim and YB Hur. 2019. Changes in water quality and hematological parameters according to the stocking density of olive flounder, Paralichthys olivaceus raised in bio-floc environment. Korean J. Environ. Biol. 37:155-163. https://doi.org/10.11626/KJEB.2019.37.2.155
  13. Kim SH, JH Kim, MA Park, SD Hwang and JC Kang. 2015. The effects of ammonia exposure on antioxidant and immune responses in Rockfish, Sabestes schlegelii during thermal stress. Environ. Toxicol. Pharmacol. 40:954-959. https://doi.org/10.1016/j.etap.2015.10.006
  14. LC Folmar. 1993. Effects of chemical contaminants on blood chemistry of teleost fish: A biblography and synopsis of selected effects. Environ. Toxicol. Chem. 12:337-375. https://doi.org/10.1897/1552-8618(1993)12[337:EOCCOB]2.0.CO;2
  15. Lee KS and HM Ryu. 2011. The acute toxicity of naphthalene on hematologic properties in juvenile flounder Paralichthys olivaceus. J. Korean Soc. Mar. Environ. 17:191-196. https://doi.org/10.7837/kosomes.2011.17.3.191
  16. Peyghan R and GA Takamy. 2002. Histopathological, serum enzyme, cholesterol and urea changes in experimental acute toxicity of ammonia in common carp Cyprinus carpio and use of natural zeolite for prevention. Aquacult. Int. 10:317-325. https://doi.org/10.1023/A:1022408529458
  17. Randall DJ and TKN Tsui. 2002. Ammonia toxicity in fish. Mar. Pollut. Bull. 45:17-23. https://doi.org/10.1016/S0025-326X(02)00227-8
  18. Riche M. 2007. Analysis of refractometry for determining total plasma protein in hybrid striped bass (Morone chrysops x M. saxatilis) at various salinities. Aquaculture 264:279-284. https://doi.org/10.1016/j.aquaculture.2006.12.018
  19. Robert MD, MC David and WB Martin. 1997. Ammonia in Fish Ponds. Publication No. 463. Southern Regional Aquaculture Center (SRAC). Stoneville, MS.
  20. Roumieh R, A Barakat, NE Abdelmeguid, J Ghanawi and IP Saoud. 2013. Acute and chronic effects of aqueous ammonia on marbled spinefoot rabbitfish, Siganus rivulatus (Forsskal 1775). Aquac. Res. 44:1777-1790. https://doi.org/10.1111/j.1365-2109.2012.03188.x
  21. Ruyet JPL, A Lamers, AL Roux, A Severe, G Boeuf and N Mayer-Gostan. 2003. Long-term ammonia exposure of turbot: effects on plasma parameters. J. Fish Biol. 62:879-894. https://doi.org/10.1046/j.1095-8649.2003.00073.x
  22. Shaheen T and T Akhtar. 2012. Assessment of chromium toxicity in Cyprinus carpio through hematological and biochemical blood markers. Turk. J. Zool. 36:682-690.
  23. Thangam Y, M Perumayee, S Jayaprakash, S Umavathi and SK Basheer. 2014. Studies of ammonia toxicity on haematological parameters to freshwater fish Cyprinus carpio (Common carp). Int. J. Curr. Microbiol. Appl. Sci. 3:535-542.
  24. Xing X, M Li, L Yuan, M Song, Q Ren, G Shi and R Wang. 2016. The protective effects of taurine on acute ammonia toxicity in grass carp Ctenopharynodon idellus. Fish Shellfish Immunol. 56:517-522. https://doi.org/10.1016/j.fsi.2016.08.005
  25. Yanagisawa T and K Hashimoto. 1984. Plasma albumins in elasmobranchs. Bull. Jpn. Soc. Sci. Fish. 50:1083-1090. https://doi.org/10.2331/suisan.50.1083
  26. Yang W, F Xiang, H Sun, Y Chen, E Minter and Z Yang. 2010. Changes in the selected hematological parameters and gill Na$^+$/K$^+$ ATPase activity of juvenile crucian carp Carassius auratus during elevated ammonia exposure and the post-exposure recovery. Biochem. Syst. Ecol. 38:557-562. https://doi.org/10.1016/j.bse.2010.06.005