Animal Systematics, Evolution and Diversity

The Korean Society of Systematic Zoology (한국동물분류학회)
권호 표지
DOI QR코드

DOI QR Code

DNA Barcoding of Antarctic Freshwater Copepod Boeckella poppei (Crustacea: Copepoda: Calanoida: Centropagidae) Inhabiting King George Island, South Shetland Islands, Antarctica

  • Received : 2020.06.19
  • Accepted : 2020.10.08
  • Published : 2020.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

The Antarctic freshwater copepod, Boeckella poppei (Mrazek, 1901), has the widest range of distribution extending from southern South America to Antarctic continent, among all Boeckella species. Boeckella poppei is the only freshwater copepod known to be inhabiting the Antarctic continent. In present study, we analyzed the DNA barcodes of the mitochondrial cytochrome c oxidase subunit I (COI) gene of B. poppei from King George Island, Antarctica. The intraspecific genetic distances varied from 0% to 13% and interspecific genetic distances ranged from 11% to 14%. The overlap of DNA barcode gap suggests careful threshold-based delimitation of species boundaries.

Keywords

Acknowledgement

This work was supported by Post-Polar Genomics Project: Functional genomic study for securing of polar useful genes (PE20040) funded by Korea Polar Research Institute.

References

  1. Aarbakke ONS, Bucklin A, Halsband C, Norrbin, F, 2014. Comparative phylogeography and demographic history of five sibling species of Pseudocalanus (Copepoda: Calanoida) in the North Atlantic Ocean. Journal of Experimental Marine Biology and Ecology, 461:479-488. https://doi.org/10.1016/j.jembe.2014.10.006
  2. Adamowicz SJ, Menu-Marque S, Hebert PDN, Purvis A, 2007. Molecular systematics and patterns of morphological evolution in the Centropagidae (Copepoda: Calanoida) of Argentina. Biological Journal of the Linnean Society, 90:279-292. https://doi.org/10.1111/j.1095-8312.2007.00723.x
  3. Bayly IAE, 1992. Fusion of the genera Boeckella and Pseudoboeckella (Copepoda) and revision of their species from South America and sub-Antarctic islands. Revista Chilena de Historia Natural, 65:17-63.
  4. Bayly IAE, Burton HR, 1993. Beaver Lake, Greater Antarctica, and its population of Boeckella poppei (Mrazek) (Copepoda: Calanoida). Internationale Vereinigung fur Theoretische und Angewandte Limnologie: Verhandlungen, 25:975-978. https://doi.org/10.1080/03680770.1992.11900300
  5. Bayly IAE, Gibson JAE, Wagner B, Swadling KM, 2003. Taxonomy, ecology and zoogeography of two East Antarctic freshwater calanoid copepod species: Boeckella poppei and Gladioferens antarcticus. Antarctic Science, 15:439-448. https://doi.org/10.1017/S0954102003001548
  6. Blanco-Bercial L, Bradford-Grieve J, Bucklin A, 2011. Molecular phylogeny of the Calanoida (Crustacea: Copepoda). Molecular Phylogenetics and Evolution, 59:103-113. https://doi.org/10.1016/j.ympev.2011.01.008
  7. Blanco-Bercial L, Cornils A, Copley N, Bucklin A, 2014. DNA barcoding of marine copepods: assessment of analytical approaches to species identification. PLoS Currents, 6:ecurrents.tol.cdf8b74881f87e3b01d56b43791626d2. https://doi.org/10.1371/currents.tol.cdf8b74881f87e3b01d56b43791626d2
  8. Cepeda GD, Blanco-Bercial L, Bucklin A, Beron CM, Vinas MD, 2012. Molecular systematic of three species of Oithona (Copepoda, Cyclopoida) from the Atlantic Ocean: comparative analysis using 28S rDNA. PLoS One, 7:e35861. https://doi.org/10.1371/journal.pone.0035861
  9. Costa FO, DeWaard JR, Boutillier J, Ratnasingham S, Dooh RT, Hajibabaei M, Hebert PDN, 2007. Biological identifications through DNA barcodes: the case of the Crustacea. Canadian Journal of Fisheries and Aquatic Sciences, 64:272-295. https://doi.org/10.1139/f07-008
  10. Gibson JAE, Bayly IAE, 2007. New insights into the origins of crustaceans of Antarctic lakes. Antarctic Science, 19:157-164. https://doi.org/10.1017/S0954102007000235
  11. Google Earth V, 2020. 7.3.3.7786 (64-bit), Barton Peninsula, King George Island, South Shetland Islands, 62°14'24" S, 58°44'36" W, Eye alt 11.20 km [Internet]. Accessed 12 Oct 2020,
  12. Heywood RB, 1977. A limnological survey of the Ablation Point area, Alexander Island, Antarctica. Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 279:39-54. https://doi.org/10.1098/rstb.1977.0070
  13. Kumar S, Stecher G, Li M, Knyaz C, Tamura K, 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35:1547-1549. https://doi.org/10.1093/molbev/msy096
  14. Machida RJ, Tsuda A, 2010. Dissimilarity of species and forms of planktonic Neocalanus copepods using mitochondrial COI, 12S, nuclear ITS, and 28S gene sequences. PLoS One, 5:e10278. https://doi.org/10.1371/journal.pone.0010278
  15. Maturana CS, Rosenfeld S, Naretto J, Convey P, Poulin E, 2019. Distribution of the genus Boeckella (Crustacea, Copepoda, Calanoida, Centropagidae) at high latitudes in South America and the main Antarctic biogeographic regions. ZooKeys, 854:1-15. https://doi.org/10.3897/zookeys.854.29614
  16. Maturana CS, Rosenfeld S, Naretto J, Convey P, Poulin E, Lopez Z, Gerard K, Contador T, Rendoll J, Jackson JA, Hong ID, Duggan IC, Bahamonde F, 2018. Occurrences of Boeckella at high latitudes in the Southern Hemisphere, version 1.1. [Internet]. Global Biodiversity Information Facility, Copenhagen, Accessed 1 Oct 2018, .
  17. Maturana CS, Segovia NI, Gonzalez-Wevar CA, Diaz A, Rosenfeld S, Poulin E, Jackson JA, Convey P, 2020. Evidence of strong small-scale population structure in the Antarctic freshwater copepod Boeckella poppei in lakes on Signy Island, South Orkney Islands. Limnology and Oceanography, 65:2024-2040. https://doi.org/10.1002/lno.11435
  18. Menu-Marque S, Morrone JJ, Locascio de Mitrovich C, 2000. Distributional patterns of the South American species of Boeckella (Copepoda: Centropagidae): a track analysis. Journal of Crustacean Biology, 20:262-272. https://doi.org/10.1163/20021975-99990038
  19. Meyer CP, Paulay G, 2005. DNA barcoding: error rates based on comprehensive sampling. PLoS Biology, 3:e422. https://doi.org/10.1371/journal.pbio.0030422
  20. Nedbalova L, Elster J, Komarek J, Nyvlt D, 2009. Lake ecosystems of James Ross Island: short characteristics. In: Structure and function of antarctic terrestrial ecosystems (Eds., Bartak M, Hajek J, Vaczi P). Muni Press, Brno, pp. 33-34.
  21. Sharma P, Gutierrez ME, Kobayashi T, 2014. Identification of common cladocerans and calanoids in two south Australian reservoirs using DNA barcoding and morphological analysis: an integrative approach. Crustaceana, 87:834-855. https://doi.org/10.1163/15685403-00003333
  22. Walter T, Boxshall G, 2018. World of Copepods database [Internet]. Accessed 20 Feb 2018, .