The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Microbiological Characteristics and Carbon source activity of Unrecorded Wild Yeast Strains from gut of the earthworm(Eisenia andrei) in Korea

지렁이(Eisenia andrei)의 장으로부터 분리한 국내 미기록 야생효모들의 균학적 특성과 탄소원 활성

  • Han, Joo Hyun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Lee, Sang Eun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • 한주현 (서울여자대학교 생명환경공학과) ;
  • 이상은 (서울여자대학교 생명환경공학과) ;
  • 김명겸 (서울여자대학교 생명환경공학과)
  • Received : 2020.04.29
  • Accepted : 2020.06.30
  • Published : 2020.06.30
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Abstract

The goal of this study was to isolate and characterize the wild yeast strains from the gut of earthworm(Eisenia andrei). The 19 yeast strains isolated from 5 gut of earthworm samples from Nanji water regeneration center in Goyang-si, Gyeonggi-do, Korea. Among them, 16 strains were recorded and 3 strains, Yarrowia deformans YP242 (=KACC48778), Sporidiobolus pararoseus YP66 (=KCTC27963) and Naganishia liquefaciens YI9 (=KACC48948) were recorded for the first time in Korea. The microbiological characteristics of these previously unrecorded yeasts were investigated. All three strains were oval-shaped, convex and smooth. However, they showed some differences in colony color and result of carbon assimilation assays. YP242 was white-colored and assimilated glycerol, L-arabinose and N-acetyl-D-glucosamine as carbon sources. YP66 was red-colored and assimilated D-Saccharose. YI9 was whitecolored and positive for 2-keto-D-gluconate assimilation.

국내에 자생하는 지렁이로(붉은줄지렁이)의 장에서 야생효모를 분리, 동정하고자 하였다. 지렁이는 고양시 난지 물 재생 센터에서 분양 받았으며, 지렁이 5마리에서 19개의 야생효모를 분리하였다. 효모의 D1/D2 영역으로 동정하여 국내에 보고되지 않은 효모 3균주를 선별하였다. 국내 미기록종 효모인 Yarrowia deformans YP242 (=KACC48778), Sporidiobolus pararoseus YP66(=KCTC27963), Naganishia liquefaciens YI9 (=KACC48948) 균주는 위상차현미경, API 20C AUX kit를 통해 균학적 특성을 조사하였다. YP242, YI9, YP66 균주의 세포 모양은 타원형이며, 집락은 볼록하고, 부드러운 재질을 가지는 것으로 관찰되었다. 탄소원 활성 측정 결과, YP242는 glycerol, L-arabinose, N-acetyl-D-glucosamine을, YP66는 N-acetyl-D-glucosamine을, liquefaciens YI9는 2-keto-D-gluconate을 탄소원으로 사용할 수 있음을 확인하였다.

Keywords

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