Korean Journal of Radiology

The Korean Society of Radiology (대한영상의학회)
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Histological Validation of Cardiovascular Magnetic Resonance T1 Mapping for Assessing the Evolution of Myocardial Injury in Myocardial Infarction: An Experimental Study

  • Lu Zhang (Department of Radiology, Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University) ;
  • Zhi-gang Yang (Department of Radiology, West China Hospital, Sichuan University) ;
  • Huayan Xu (Department of Radiology, Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University) ;
  • Meng-xi Yang (Department of Radiology, West China Hospital, Sichuan University) ;
  • Rong Xu (Department of Radiology, Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University) ;
  • Lin Chen (Department of Radiology, Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University) ;
  • Ran Sun (Key Laboratory of Obstetrics & Gynecology and Pediatric Disease and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University) ;
  • Tianyu Miao (Vascular Surgery, West China Hospital, Sichuan University) ;
  • Jichun Zhao (Vascular Surgery, West China Hospital, Sichuan University) ;
  • Xiaoyue Zhou (Siemens Healthineers Ltd.) ;
  • Chuan Fu (Department of Radiology, West China Second Hospital, Sichuan University) ;
  • Yingkun Guo (Department of Radiology, Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan University)
  • Received : 2020.02.11
  • Accepted : 2020.04.24
  • Published : 2020.12.01
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Abstract

Objective: To determine whether T1 mapping could monitor the dynamic changes of injury in myocardial infarction (MI) and be histologically validated. Materials and Methods: In 22 pigs, MI was induced by ligating the left anterior descending artery and they underwent serial cardiovascular magnetic resonance examinations with modified Look-Locker inversion T1 mapping and extracellular volume (ECV) computation in acute (within 24 hours, n = 22), subacute (7 days, n = 13), and chronic (3 months, n = 7) phases of MI. Masson's trichrome staining was performed for histological ECV calculation. Myocardial native T1 and ECV were obtained by region of interest measurement in infarcted, peri-infarct, and remote myocardium. Results: Native T1 and ECV in peri-infarct myocardium differed from remote myocardium in acute (1181 ± 62 ms vs. 1113 ± 64 ms, p = 0.002; 24 ± 4% vs. 19 ± 4%, p = 0.031) and subacute phases (1264 ± 41 ms vs. 1171 ± 56 ms, p < 0.001; 27 ± 4% vs. 22 ± 2%, p = 0.009) but not in chronic phase (1157 ± 57 ms vs. 1120 ± 54 ms, p = 0.934; 23 ± 2% vs. 20 ± 1%, p = 0.109). From acute to chronic MI, infarcted native T1 peaked in subacute phase (1275 ± 63 ms vs. 1637 ± 123 ms vs. 1471 ± 98 ms, p < 0.001), while ECV progressively increased with time (35 ± 7% vs. 46 ± 6% vs. 52 ± 4%, p < 0.001). Native T1 correlated well with histological findings (R2 = 0.65 to 0.89, all p < 0.001) so did ECV (R2 = 0.73 to 0.94, all p < 0.001). Conclusion: T1 mapping allows the quantitative assessment of injury in MI and the noninvasive monitoring of tissue injury evolution, which correlates well with histological findings.

Keywords

Acknowledgement

This work was supported by the National Natural Science Foundation of China (81471721, 81471722, 81641169, 81771887, 81771897, 81901712, and 81971586); the Program for New Century Excellent Talents in University (No. NCET-13-0386); the Program for Young Scholars and Innovative Research Team in Sichuan Province (No. 2017TD0005) of China; the Applied and Fundamental Study of Sichuan Province (No. 2017JY0026) and 1·3·5 project for disciplines of excellence, West China Hospital, Sichuan University (No. ZYGD18013).

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