Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Reanalysis of discarded blastocysts for autosomal aneuploidy after sex selection in cleavage-stage embryos

  • Ebrahimian, Neda (Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences) ;
  • Montazeri, Fatemeh (Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences) ;
  • Sadeghi, Mohammad Reza (Reproductive Embryology and Andrology Research Center, Avicenna Research Institute, Academic Center for Education, Culture and Research) ;
  • Kalantar, Seyed Mehdi (Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences) ;
  • Gilany, Kambiz (Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR) ;
  • Khalili, Mohannad Ali (Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences)
  • Received : 2019.11.21
  • Accepted : 2020.06.11
  • Published : 2020.12.31
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Abstract

Objective: The goal of the present study was to investigate the rate of chromosomal aneuploidies in surplus embryos after sex determination at the cleavage stage. Then, the same chromosomal aneuploidies were evaluated in blastocysts after extended culture. Methods: Sixty-eight surplus embryos were biopsied at the cleavage stage and incubated for an additional 3 days to allow them to reach the blastocyst stage. The embryos were reanalyzed via fluorescence in situ hybridization (FISH) to examine eight chromosomes (13, 15, 16, 18, 21, 22, X, and Y) in both cleavage-stage embryos and blastocysts. Results: Although the total abnormality rate was lower in blastocysts (32.35%) than in cleavage-stage embryos (45.58%), the difference was not significant (p=0.113). However, when we restricted the analysis to autosomal abnormalities, we observed a significant difference in the abnormality rate between the cleavage-stage embryos (44.11%) and the blastocysts (17.64%, p=0.008). A higher rate of sex chromosomal abnormalities was also observed in cleavage-stage embryos (29.4%) than in blastocysts (14.70%, p=0.038). Conclusion: The data indicated that embryo biopsy should be conducted at the blastocyst stage rather than the cleavage stage. The results also emphasized that examination of common chromosomal aneuploidies apart from sex selection cycles can be conducted in the blastocyst stage with the FISH method.

Keywords

Acknowledgement

We would like to thank the staff of Assisted Reproductive Technologies at the Avicenna Infertility Center in Tehran and the staff of the Abortion Research Center at the Yazd Reproductive Sciences Institute in Yazd, Iran.

References

  1. Daughtry BL, Chavez SL. Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences. Cell Tissue Res 2016;363:201-25.
  2. Lagalla C, Tarozzi N, Sciajno R, Wells D, Di Santo M, Nadalini M, et al. Embryos with morphokinetic abnormalities may develop into euploid blastocysts. Reprod Biomed Online 2017;34:137-46.
  3. Gianaroli L, Magli MC, Ferraretti AP, Lappi M, Borghi E, Ermini B. Oocyte euploidy, pronuclear zygote morphology and embryo chromosomal complement. Hum Reprod 2007;22:241-9.
  4. Daughtry BL, Rosenkrantz JL, Lazar NH, Fei SS, Redmayne N, Torkenczy KA, et al. Single-cell sequencing of primate preimplantation embryos reveals chromosome elimination via cellular fragmentation and blastomere exclusion. Genome Res 2019;29:367-82.
  5. Scott RT Jr, Upham KM, Forman EJ, Hong KH, Scott KL, Taylor D, et al. Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery rates: a randomized controlled trial. Fertil Steril 2013;100:697-703.
  6. Fragouli E, Bianchi V, Patrizio P, Obradors A, Huang Z, Borini A, et al. Transcriptomic profiling of human oocytes: association of meiotic aneuploidy and altered oocyte gene expression. Mol Hum Reprod 2010;16:570-82.
  7. Sahin L, Bozkurt M, Sahin H, Gurel A, Yumru AE. Is preimplantation genetic diagnosis the ideal embryo selection method in aneuploidy screening? Kaohsiung J Med Sci 2014;30:491-8.
  8. Franasiak JM, Forman EJ, Hong KH, Werner MD, Upham KM, Treff NR, et al. The nature of aneuploidy with increasing age of the female partner: a review of 15,169 consecutive trophectoderm biopsies evaluated with comprehensive chromosomal screening. Fertil Steril 2014;101:656-63.
  9. Munne S, Chen S, Colls P, Garrisi J, Zheng X, Cekleniak N, et al. Maternal age, morphology, development and chromosome abnormalities in over 6000 cleavage-stage embryos. Reprod Biomed Online 2007;14:628-34.
  10. Lee E, Illingworth P, Wilton L, Chambers GM. The clinical effectiveness of preimplantation genetic diagnosis for aneuploidy in all 24 chromosomes (PGD-A): systematic review. Hum Reprod 2015;30:473-83.
  11. Donoso P, Staessen C, Fauser BC, Devroey P. Current value of preimplantation genetic aneuploidy screening in IVF. Hum Reprod Update 2007;13:15-25.
  12. Harton GL, Magli MC, Lundin K, Montag M, Lemmen J, Harper JC, et al. ESHRE PGD Consortium/Embryology Special Interest Group: best practice guidelines for polar body and embryo biopsy for preimplantation genetic diagnosis/screening (PGD/PGS). Hum Reprod 2011;26:41-6.
  13. Sachdev NM, Maxwell SM, Besser AG, Grifo JA. Diagnosis and clinical management of embryonic mosaicism. Fertil Steril 2017;107:6-11.
  14. Zakharova EE, Zaletova VV, Krivokharchenko AS. Biopsy of human morula-stage embryos: outcome of 215 IVF/ICSI cycles with PGS. PLoS One 2014;9:e106433.
  15. Fesahat F, Kalantar SM, Sheikhha MH, Saeedi H, Montazeri F, Firouzabadi RD, et al. Developmental and cytogenetic assessments of preimplantation embryos derived from in-vivo or in-vitro matured human oocytes. Eur J Med Genet 2018;61:235-41.
  16. De Rycke M, Goossens V, Kokkali G, Meijer-Hoogeveen M, Coonen E, Moutou C. ESHRE PGD Consortium data collection XIV-XV: cycles from January 2011 to December 2012 with pregnancy follow-up to October 2013. Hum Reprod 2017;32:1974-94.
  17. Anbari F, Halvaei I, Nabi A, Ghazali S, Khalili MA, Johansson L. The quality of sperm preparation medium affects the motility, viability, and DNA integrity of human spermatozoa. J Hum Reprod Sci 2016;9:254-8.
  18. World Health Organization. Sperm preparation techniques. In: World Health Organization, editor. WHO laboratory manual for the examination and processing of human semen. Geneva: World Health Organization; 2010. p. 160-68.
  19. Sakkas D, Gardner DK. Evaluation of embryo quality analysis of morphology and physiology. In: Gardner DK, Weissman A, Howles CM, Shoham Z. editors. Textbook of assisted reproductive techniques. 5th ed. Boca Raton: CRC Press; 2017. p. 226-43.
  20. Magli MC, Jones GM, Lundin K, van den Abbeel E. Atlas of human embryology: from oocytes to preimplantation embryos. Preface. Hum Reprod 2012;27 Suppl 1:i1.
  21. Singh S, Hobeika E, Knochenhauer ES, Traub ML. Pregnancy rates after pre-implantation genetic screening for aneuploidy are only superior when trophectoderm biopsy is performed on hatching embryos. J Assist Reprod Genet 2019;36:621-8.
  22. Barbash-Hazan S, Frumkin T, Malcov M, Yaron Y, Cohen T, Azem F, et al. Preimplantation aneuploid embryos undergo self-correction in correlation with their developmental potential. Fertil Steril 2009;92:890-6.
  23. Kort DH, Chia G, Treff NR, Tanaka AJ, Xing T, Vensand LB, et al. Human embryos commonly form abnormal nuclei during development: a mechanism of DNA damage, embryonic aneuploidy, and developmental arrest. Hum Reprod 2016;31:312-23.
  24. Delhanty JD. The origins of genetic variation between individual human oocytes and embryos: implications for infertility. Hum Fertil (Camb) 2013;16:241-5.
  25. Liang L, Wang CT, Sun X, Liu L, Li M, Witz C, et al. Identification of chromosomal errors in human preimplantation embryos with oligonucleotide DNA microarray. PLoS One 2013;8:e61838.
  26. Fragouli E, Alfarawati S, Spath K, Jaroudi S, Sarasa J, Enciso M, et al. The origin and impact of embryonic aneuploidy. Hum Genet 2013;132:1001-13.
  27. Capalbo A, Bono S, Spizzichino L, Biricik A, Baldi M, Colamaria S, et al. Sequential comprehensive chromosome analysis on polar bodies, blastomeres and trophoblast: insights into female meiotic errors and chromosomal segregation in the preimplantation window of embryo development. Hum Reprod 2013;28:509-18.
  28. Munne S, Grifo J, Wells D. Mosaicism: "survival of the fittest" versus "no embryo left behind". Fertil Steril 2016;105:1146-9.
  29. Zhang XY, Ata B, Son WY, Buckett WM, Tan SL, Ao A. Chromosome abnormality rates in human embryos obtained from in-vitro maturation and IVF treatment cycles. Reprod Biomed Online 2010;21:552-9.
  30. Eaton JL, Hacker MR, Harris D, Thornton KL, Penzias AS. Assessment of day-3 morphology and euploidy for individual chromosomes in embryos that develop to the blastocyst stage. Fertil Steril 2009;91:2432-6.
  31. Turner K, Fowler K, Fonseka G, Griffin D, Ioannou D. Multicolor detection of every chromosome as a means of detecting mosaicism and nuclear organization in human embryonic nuclei. Panminerva Med 2016;58:175-90.