Safety and Health at Work

Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency (산업안전보건연구원)
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A Study on Concentration, Identification, and Reduction of Airborne Microorganisms in the Military Working Dog Clinic

  • Kim, Min-Ho (Department of Work Environment Monitoring, Armed Forces Medical Research Institute) ;
  • Baek, Ki-Ook (Department of Work Environment Monitoring, Armed Forces Medical Research Institute) ;
  • Park, Gyeong-Gook (Department of Research of Infectious Disease, Armed Forces Medical Research Institute) ;
  • Jang, Je-Youn (Department of Veterinary Medicine, Armed Forces Medical Research Institute) ;
  • Lee, Jin-Hong (Department of Environmental Engineering, Chungnam National University of Engineering)
  • Received : 2020.06.02
  • Accepted : 2020.09.01
  • Published : 2020.12.30
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Abstract

Background: The study was planned to show the status of indoor microorganisms and the status of the reduction device in the military dog clinic. Methods: Airborne microbes were analyzed according to the number of daily patient canines. For identification of bacteria, sampled bacteria was identified using VITEK®2 and molecular method. The status of indoor microorganisms according to the operation of the ventilation system was analyzed. Results: Airborne bacteria and fungi concentrations were 1000.6 ± 800.7 CFU/m3 and 324.7 ± 245.8 CFU/m3. In the analysis using automated identification system, based on fluorescence biochemical test, VITEK®2, mainly human pathogenic bacteria were identified. The three most frequently isolated genera were Kocuria (26.6%), Staphylococcus (24.48%), and Granulicatella (12.7%). The results analyzed by molecular method were detected in the order of Kocuria (22.6%), followed by Macrococcus (18.1%), Glutamicibacter (11.1%), and so on. When the ventilation system was operated appropriately, the airborne bacteria and fungi level were significantly decreased. Conclusion: Airborne bacteria in the clinic tend to increase with the number of canines. Human pathogenic bacteria were mainly detected in VITEK®2, and relatively various bacteria were detected in molecular analysis. A decrease in the level of bacteria and fungi was observed with proper operation of the ventilation system.

Keywords

Acknowledgement

This research was not supported by any funding source.

References

  1. Bell DR, Berghaus RD, Patel S, Beavers S, Fernandez I, Sanchez S. Seroprevalence of tick-borne infections in military working dogs in the Republic of Korea. Vector Borne Zoonotic Dis 2012;12:1023-30.
  2. Harper TA, Bridgewater S, Brown L, Pow-Brown P, Stewart-Johnson A, Adesiyun AA. Bioaerosol sampling for airborne bacteria in a small animal veterinary teaching hospital. Infect Ecol Epidemiol 2013;3:20376.
  3. Chen C, Liu B, Hsu C, Liu C, Liao AT, Chou C, Lin C. Bioaerosol investigation in three veterinary teaching hospitals in taiwan. Taiwan Vet J 2017;43:39-45.
  4. Weese J, Peregrine A, Armstrong J. Occupational health and safety in small animal veterinary practice: Part I-nonparasitic zoonotic diseases. Can Vet J 2002;43:631.
  5. Bang K, An JU, Kim W, Dong HJ, Kim J, Cho S. Antibiotic resistance patterns and genetic relatedness of Enterococcus faecalis and Enterococcus faecium isolated from military working dogs in Korea. J Vet Sci 2017;18:229-36.
  6. Heederik D, Brouwer R, Biersteker K, Boleij JS. Relationship of airborne endotoxin and bacteria levels in pig farms with the lung function and respiratory symptoms of farmers. Int Arch Occup Environ Health 1991;62:595-601.
  7. bioMerieux Industry Culture Media Group. Physical Efficiency Testing of the bioMerieux air IDEAL 3P ® sampler followingnthe ISO 14698-1 standard (Marcy L'etoile) [Internet]. bioMerieux. 2005 [cited 2020 Jul 26]. Available from: http://https://www.biomerieux-usa.com/sites/subsidiary_us/files/doc/airideal-physical-efficiency-testing-1.pdf.
  8. Ligozzi M, Bernini C, Bonora MG, de Fatima M, Zuliani J, Fontana R. Evaluation of the VITEK 2 system for identification and antimicrobial susceptibility testing of medically relevant gram-positive cocci. J Clin Microbiol 2002;40:1681-6.
  9. Singh UA, Kumari M, Iyengar S. Method for improving the quality of genomic DNA obtained from minute quantities of tissue and blood samples using Chelex 100 resin. Biol Proced Online 2018;20:12.
  10. Mandal J, Brandl H. Bioaerosols in indoor environment-a review with special reference to residential and occupational locations. Open Environ Biol Monit J 2011;4.
  11. Dutkiewicz J, Pomorski ZJH, Sitkowska J, Krysinska-Traczyk E, Skorska C, Prazmo Z, Cholewa G, Wojtowicz H. Airborne microorganisms and endotoxin _ in animal houses. Grana 1994;33:85-90.
  12. Kim KY, Ko HJ. Indoor distribution characteristics of airborne bacteria in pig buildings as influenced by season and housing type. Asian-Australas J Anim Sci 2019;32:742.
  13. Jeon B-H, Hwang I-Y. Concentrations of total culturable microorganisms and its identification in public facilities. JKAIS 2015;16:868-76.
  14. Kim KY, Kim CN. Airborne microbiological characteristics in public buildings of Korea. Build Sci 2007;42:2188-96.
  15. Kim KY, Kim HT, Kim D, Nakajima J, Higuchi T. Distribution characteristics of airborne bacteria and fungi in the feedstuff-manufacturing factories. J Hazard Mater 2009;169:1054-60.
  16. Wolmarans E, du Preez HH, de Wet CM, Venter SN. Significance of bacteria associated with invertebrates in drinking water distribution networks. Water Sci Technol 2005;52:171-5.
  17. Delmas J, Chacornac JP, Robin F, Giammarinaro P, Talon R, Bonnet R. Evaluation of the Vitek 2 system with a variety of Staphylococcus species. J Clin Microbiol 2008;46:311-3.
  18. Ma ES, Wong CL, Lai KT, Chan EC, Yam WC, Chan AC. Kocuria kristinae infection associated with acute cholecystitis. BMC Infect Dis 2005;5:60.
  19. Maslanova I, Wertheimer Z, Sedla cek I, Svec P, Indrakova A, Kovarovic V, Schumann P, Sproer C, Kralova S, Sedo O, Kristofova L, Vrbovska V, Fuzik T, Petras P, Zdrahal Z, Ruzickova V, Doskar J, Pantucek R. Kocuria rhizophila adds to the emerging spectrum of micrococcal species involved in human infections. J Clin Microbiol 2008;46:3537-9.
  20. Altuntas F, Yildiz O, Eser B, Gundogan K, Sumerkan B, Cetin M. Catheterrelated bacteremia due to Kocuria rosea in a patient undergoing peripheral blood stem cell transplantation. BMC Infect Dis 2004;4:1-3.
  21. Tsai C-Y, Su S-h, Cheng Y-H, Chou Y-l, Tsai T-H, Lieu A-S. Kocuria varians infection associated with brain abscess: a case report. BMC Infect Dis 2010;10:1-4.
  22. Peer M, Nasir R, Kakru D, Fomda B, Bashir G, Sheikh I. Sepsis due to linezolid resistant Staphylococcus cohnii and Staphylococcus kloosii: first reports of linezolid resistance in coagulase negative staphylococci from India. Indian J Med Microbiol 2011;29:60.
  23. Karachalios GN, Michelis FV, Kanakis KV, Karachaliou I, Koutri R, Zacharof AK. Splenic abscess due to Staphylococcus lentus: a rare entity. Scand J Infect Dis 2006;38:708-10.
  24. Stepanovic S, Jezek P, Vukovic D, Dakic I, Petras P. Isolation of members of the Staphylococcus sciuri group from urine and their relationship to urinary tract infections. J Clin Microbiol 2003;41:5262-4.
  25. Abdul-Redha RJ, Prag J, Sonksen UW, Kemp M, Andresen K, Christensen JJ. Granulicatella elegans bacteraemia in patients with abdominal infections. Scand J Infect Dis 2007;39:830-3.
  26. Seifert H, Kaltheuner M, Perdreau-Remington F. Micrococcus luteus endocarditis: case report and review of the literature. Zentralblatt fur Bakteriologie. 1995;282:431-5.
  27. Maslanova I, Wertheimer Z, Sedla cek I, Svec P, Indrakova A, Kovarovic V, et al. Description and comparative genomics of Macrococcus caseolyticus subsp. hominis subsp. nov., Macrococcus goetzii sp. nov., Macrococcus epidermidis sp. nov., and Macrococcus bohemicus sp. nov., novel macrococci from human clinical material with virulence potential and suspected uptake of foreign DNA by natural transformation. Front Microbiol 2018;9:1178.
  28. Brawand SG, Cotting K, Gomez-Sanz E, Collaud A, Thomann A, Brodard I, Rodriguez-Campos S, Strauss C, Perreten V. Macrococcus canis sp. nov., a skin bacterium associated with infections in dogs. Int J Syst Evol 2017;67:621-6.
  29. Santos RG, Hurtado R, Gomes LGR, Profeta R, Rifici C, Attili AR, Spier SJ, Mazzullo G, Rodrigues FM, Gomide ACP, Brenigh B, Garcia AG, Cuteri V, Castro TLP, Ghoshi P, Seyffert N, Azevedo V. Complete genome analysis of Glutamicibacter creatinolyticus from mare abscess and comparative genomics provide insight of diversity and adaptation for Glutamicibacter. Gene 2020;741:144566.
  30. Dunleavy J. Curtobacterium plantarum sp. nov. is ubiquitous in plant leaves and is seed transmitted in soybean and corn. Int J Syst Evol 1989;39:240-9.
  31. Kembel SW, Jones E, Kline J, Northcutt D, Stenson J, Womack AM, Bohannan BJ, Brown GZ, Green JL. Architectural design influences the diversity and structure of the built environment microbiome. ISME J 2012;6:1469-79.
  32. Kodama AM, McGee RI. Airborne microbial contaminants in indoor environments. Naturally ventilated and air-conditioned homes. Arch Environ Health 1986;41:306-11.
  33. Kulmala M, Asmi A, Pirjola L. Indoor air aerosol model: the effect of outdoor air, filtration and ventilation on indoor concentrations. Atmos Environ 1999;33:2133-44.
  34. Meadow JF, Altrichter AE, Kembel SW, Kline J, Mhuireach G, Moriyama M, Northcutt D, O'Connor TK, Womack AM, Brown GZ, Green JL, Bohannan BJM. Indoor airborne bacterial communities are influenced by ventilation, occupancy, and outdoor air source. Indoor Air 2014;24:41-8.
  35. Joo Y-C, Kim C-S, Kwon S-J. A study on the air ventilation system of operation room for the prevention of hospital infection. JKAIS 2004;5:552-7.