Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Study on the Stability of Biotin-containing Nano-liposome

바이오틴 함유 나노리포좀의 안정성에 관한 연구

  • Yang, Seong Jun (Department of Chemistry and Cosmetics, College of National Science, Jeju National University) ;
  • Kim, Tae Yang (Songpa R&D Center, Coreana Cosmetic CO., Ltd.) ;
  • Lee, Chun Mong (Songpa R&D Center, Coreana Cosmetic CO., Ltd.) ;
  • Lee, Kwang Sik (Songpa R&D Center, Coreana Cosmetic CO., Ltd.) ;
  • Yoon, Kyung-Sup (Department of Chemistry and Cosmetics, College of National Science, Jeju National University)
  • 양성준 (제주대학교 화학.코스메틱학과) ;
  • 김태양 ((주)코리아나화장품 송파기술연구원) ;
  • 이춘몽 ((주)코리아나화장품 송파기술연구원) ;
  • 이광식 ((주)코리아나화장품 송파기술연구원) ;
  • 윤경섭 (제주대학교 화학.코스메틱학과)
  • Received : 2020.04.02
  • Accepted : 2020.05.27
  • Published : 2020.06.30
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Abstract

This study utilized nano-liposomes for the purpose of stabilizing and increasing the solubility of biotin, a water-soluble active material with low solubility. The particle size, zeta potential, and polydispersity index were confirmed with a nano zetasizer. It was possible to manufacture nano liposomes at 100 to 250 nm of particle size and -80 to -30 mV of zeta potential. Dialysis membrane method (DMM) was used to measure the capsulation efficiency of biotin in biotin nano-liposomes, and results showed that pH increased biotin nano-liposomes had higher capsulation efficiency than normal biotin nano-liposome. Through this experiment, it was confirmed that the pH has a great influence on the stability of biotin nano-liposomes. In vitro franz diffusion cell method was used to measure in vitro skin absorption rate of biotin nano-liposomes. The shape of the formulation and biotin solubility in nano-liposome was observed by cryogenic transmission electron microscopy (cryo-TEM). Through this study, we confirmed that biotin, which is introduced as closely related to hair health, can be incorporated into a nano-liposome drug delivery system, to make biotin nano-liposome with improved solubility and precipitation problems.

본 연구는 용해도가 낮은 수용성 활성물질인 바이오틴(biotin)의 안정화 및 용해도 증가를 목적으로 나노리포좀을 활용하였다. 이번 실험을 통해 바이오틴 나노리포좀의 안정성에 pH가 큰 영향을 준다는 사실을 확인할 수 있었으며, pH 상승이 바이오틴 활성에 튼 영향을 미치지 않음을 확인하였다. 또한 제타사이저(zetasizer)로 입자크기, 제타전위(zeta potential) 및 다분산지수(polydispersity index)를 측정하여 안정성을 평가하였다. 입자크기는 평균 100 ~ 250 nm, 제타전위 -80 ~ -30 mV로 나노리포좀 제조가 가능함을 확인하였다. 바이오틴 나노리포좀 내의 바이오틴 캡슐화율(capsulation efficiency)을 측정하기 위해 dialysis membrane method (DMM)를 이용하여 평가하였으며, 이를 통해 알지닌을 첨가시킨 바이오틴 나노리포좀이 일반 바이오틴 나노리포좀보다 캡슐화율이 5 배 높은 것으로 측정되었다. 바이오틴 나노리포좀의 경피흡수율을 측정하기 위해 in vitro franz diffusion cell method를 통해 확인하였으며, cryogenic transmission electron microscopy (cryo - TEM)을 통해 바이오틴 나노리포좀이 잘 형성되었는지 확인하였다. 본 논문을 통하여 모발건강과 밀접한 관계가 있는 것으로 소개된 바이오틴을 약물전달체(drug delivery carrier)인 나노리포좀에 캡슐화시켜 기존의 낮은 용해도 및 석출되는 문제를 보완한 바이오틴 나노리포좀을 만들 수 있음을 확인하였다.

Keywords

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