The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Ameliorative Effect of Schisandra chinensis and Ribes fasciculatum Extracts on Hydrogen Peroxide-Induced Neuronal Cell Death in Neuroblastic PC12 Cells and the Scopolamine-Induced Cognitive Impairment in a Rat Model

오미자칠해목 추출물의 과산화수소로 유발된 PC12뇌세포 사멸과 스코폴라민으로 유발된 렛드 동물모델에 대한 개선 효과

  • 박은국 (아주대학교 의과대학 의학유전학과) ;
  • 한경훈 (서울의료원 의학연구소) ;
  • 허재혁 (서울의료원 신경과) ;
  • 김남기 (알피바이오(주) 연구소) ;
  • 배문형 (알피바이오(주) 연구소) ;
  • 서영하 (알피바이오(주) 연구소) ;
  • 용윤중 ((주)나인비 연구소) ;
  • 정선용 ((주)나인비) ;
  • 최춘환 ((재)경기도경제과학진흥원 바이오센터 천연물연구팀)
  • Received : 2020.05.07
  • Accepted : 2020.06.11
  • Published : 2020.06.30
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Abstract

Cognitive impairment is considered to be key research topics in the field of neurodegenerative diseases and in understanding of learning and memory. In the present study, we investigated neuroprotective effects of Schisandra chinensis (SC) and Ribes fasciculatum (RF) extracts in hydrogen peroxide-induced neuronal cell death in vitro and scopolamine-induced cognitive impairment in Sprague Dawley® (SD) rat in vivo. Apoptotic cell death in neuroblastic PC12 cell line was induced by hydrogen peroxide for 1 hour at 100 μM. However, mixture of SC and RF treatment prevented peroxide induced PC12 cell death with no neurotoxic effects. For in vivo experiment, the effect of SC and RF extracts on scopolamine-induced cognitive impairment in SD rat was evaluated by spontaneous alternation behavior in Y-Maze test. After 30 min scopolamine injection, the scopolamine-induced rats presented significantly decreased % spontaneous alteration and acetylcholine level, compared to non-induced group. However, treatment of SC+RF extracts rescued the reduced % spontaneous alteration with acetylcholine concentration from hippocampus in scopolamine-induced rats. These results suggested that mixture of SC and RF extract may be a potential natural therapeutic agent for the prevention of cognitive impairment.

Keywords

References

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