Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-inflammatory effect of Malus domestica cv. Green ball apple peel extract on Raw 264.7 macrophages

  • Lee, Eun-Ho (School of Food science & Biotechnology, Kyungpook National University) ;
  • Park, Hye-Jin (School of Food science & Biotechnology, Kyungpook National University) ;
  • Kim, Byung-Oh (School of Food science & Biotechnology, Kyungpook National University) ;
  • Choi, Hyong-Woo (Department of Plant Medicals, Andong National University) ;
  • Park, Kyeung-Il (Department of Horticulture and Life Science, Yeungnam University) ;
  • Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University) ;
  • Cho, Young-Je (School of Food science & Biotechnology, Kyungpook National University)
  • Received : 2020.02.05
  • Accepted : 2020.03.17
  • Published : 2020.06.30
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Abstract

We examined the anti-inflammatory effect of the peel extract of the newly bred Korean apple (Malus domestica Borkh.) cultivar Green ball. To test its possible use as anti-inflammatory functional material, Raw 264.7 macrophages were treated with pro-inflammatory lipopolysaccharide (LPS) in the presence or absence of Green ball apple peel ethanol extract (GBE). Notably, up to 500 ㎍/mL of GBE did not result in any signs of inhibition on cellular metabolic activity or cytotoxicity in Raw 264.7 macrophages. Supplementation with GBE to LPS-treated Raw 264.7 macrophage significantly suppressed various pro-inflammatory responses in a dose-dependent manner, including i) nitric oxide (NO) production, ii) accumulation of inducible NO synthase and cyclooxygenase-2, iii) phosphorylation of nuclear factor-kappa B (NF-κB) subunit p65, and iv) expression of pro-inflammatory biomarker genes, including tumor necrosis factor alpha, interleukin 1 beta, interleukin 6, monocyte chemoattractant protein-1, and prostaglandin E synthase 2.

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