Korean Journal of Radiology

The Korean Society of Radiology (대한영상의학회)
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Role of Chemical Exchange Saturation Transfer and Magnetization Transfer MRI in Detecting Metabolic and Structural Changes of Renal Fibrosis in an Animal Model at 3T

  • Anqin Li (Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Chuou Xu (Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Ping Liang (Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Yao Hu (Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Yaqi Shen (Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Daoyu Hu (Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Zhen Li (Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Ihab R. Kamel (Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins Medical Institutions)
  • Received : 2019.08.29
  • Accepted : 2019.12.08
  • Published : 2020.05.01
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Abstract

Objective: To investigate the value of combined chemical exchange saturation transfer (CEST) and conventional magnetization transfer imaging (MT) in detecting metabolic and structural changes of renal fibrosis in rats with unilateral ureteral obstruction (UUO) at 3T MRI. Materials and Methods: Thirty-five Sprague-Dawley rats underwent UUO surgery (n = 25) or sham surgery (n = 10). The obstructed and contralateral kidneys were evaluated on days 1, 3, 5, and 7 after surgery. After CEST and MT examinations, 18F-labeled fluoro-2-deoxyglucose positron emission tomography was performed to quantify glucose metabolism. Fibrosis was measured by histology and western blots. Correlations were compared between asymmetrical magnetization transfer ratio at 1.2 ppm (MTRasym(1.2ppm)) derived from CEST and maximum standard uptake value (SUVmax) and between magnetization transfer ratio (MTR) derived from MT and alpha-smooth muscle actin (α-SMA). Results: On days 3 and 7, MTRasym(1.2ppm) and MTR of UUO renal cortex and medulla were significantly different from those of contralateral kidneys (p < 0.05). On day 7, MTRasym(1.2ppm) and MTR of UUO renal cortex and medulla were significantly different from those of sham-operated kidneys (p < 0.05). The MTRasym(1.2ppm) of UUO renal medulla was fairly negatively correlated with SUVmax (r = -0.350, p = 0.021), whereas MTR of UUO renal medulla was strongly negatively correlated with α-SMA (r = -0.744, p < 0.001). Conclusion: CEST and MT could provide metabolic and structural information for comprehensive assessment of renal fibrosis in UUO rats in 3T MRI and may aid in clinical monitoring of renal fibrosis in patients with chronic kidney disease.

Keywords

Acknowledgement

The authors thank Xiaolei Song for MRI technical assistance (Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins Medical Institutions, Baltimore, MD, USA).

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