한국산림과학회지 (Journal of Korean Society of Forest Science)

  • 제109권2호
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  • Pages.189-194
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  • 2020
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  • 2586-6613(pISSN)
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  • 2586-6621(eISSN)
한국산림과학회 (Korean Society of Forest Science)
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멸종위기종 두메닥나무(Daphne pseudomezereum var. koreana)의 줄기 기내배양을 통한 식물체 생산

Propagation of Endangered Species, Daphne pseudomezereum var. koreana via in vitro Bud Culture

  • 추예린 (강원대학교 산림환경시스템학과) ;
  • 박상희 (강원대학교 산림환경시스템학과) ;
  • 정은주 (강원대학교 산림환경시스템학과)
  • Chu, Yerin (Department of Forest and Environment System, Kangwon National University) ;
  • Park, Sanghee (Department of Forest and Environment System, Kangwon National University) ;
  • Cheong, Eun Ju (Department of Forest and Environment System, Kangwon National University)
  • 투고 : 2020.03.03
  • 심사 : 2020.04.08
  • 발행 : 2020.06.30
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초록

두메닥나무 (Daphne pseudomezereum var. koreana)는 강원도, 전라북도, 경상도의 고산지대에 분포하는 한국 자생종이다. 최근 자생지가 축소되어 개체 수가 급격히 줄어들고 있어 멸종위기종으로 지정되었다. 두메닥나무의 추출물은 화장품 등에 유용한 소재로 이용되나 식물체의 번식 효율은 매우 낮은 실정이다. 본 연구는 두메닥나무의 유전자원 보존과 생물소재로 활용을 위해 대량증식법을 확립하고자 하였다. 온실에서 자란 두메닥나무의 액아를 포함한 줄기절편을 생장이 우수했던 WPM 배지를 기본 조성으로 하여 BA와 GA3를 혼용 처리한 4가지 배지에서 기내줄기의 정단의 유무에 따른 줄기생장과 신초유도를 비교하였다. 두메닥나무는 정아가 포함된 절편체는 BA 8μM + GA38μM 조성에서 줄기생장이 가장 우수하였으며, 정아가 존재하지 않을 때 BA 8μM + GA38μM 조성에서 신초유도가 가장 우수하였다. 반면 발근유도는 NAA 1μM, NAA 5μM, NAA 10μM 조성 모두 발근이 발생하지 않은 대신 잎과 줄기가 생장하여 효과가 없는 것으로 나타났다.

Daphne pseudomezereum var. koreana is native to Korea and is distributedin Kangwon-do, Jeollabuk do, and Gyeongsang-do. This economically valuable species has experienced a dramatic decrease in natural habitat due to climate change and is difficult to cultivate. In this study, we investigate a mass propagation method for D. pseudomezereum through in vitro culture and genetic resource preservation.WPM medium was better than the MS medium for shoot growth. As a result, we compared the shoot number and length of apical (W/AP) and non-apical shoots (W0/AP) with BA and GA3 treatments in WPM medium. Their shoots and length grew well in both BA 8ìM + GA38ìM-treated apical shoot and without-apical shoot. NAA did not effectively induce rooting of the in vitro plantlet.

키워드

참고문헌

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