Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Effects of Temperature and Light Intensity on the Early Growth of Tetrasporophytes and Gametophytes of Agarophyton vermiculophyllum

꼬시래기의 사분포자체와 배우체의 초기 생장에 대한 온도와 광도의 영향

  • Lee, Sang Yong (Seaweed Research Center, National Institute of Fisheries Science) ;
  • Choi, Han Gil (Faculty of Biological Science and Institute of Basic Natural Sciences, College of Natural Sciences, Wonkwang University)
  • 이상용 (국립수산과학원 해조류연구센터) ;
  • 최한길 (원광대학교 자연과학대학 생명과학부.기초자연과학연구소)
  • Received : 2020.01.13
  • Accepted : 2020.05.18
  • Published : 2020.06.30
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Abstract

The aim of this study is to examine the physiological characteristics of an agarophyte Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N. Norris et Fredericq in the early life stage of tetrasporophytes (2n) and gametophytes (n) to select appropriate seedlings for mariculture. Growth experiments were carried out at the combinations of four temperatures (20, 25, 30, and 35℃) and three light intensity levels (20, 60, and 100 µmol photons m-2 s-1) in the two ontogenetic stages: discoid holdfasts and erect sporelings. Holdfast areas and sporeling lengths of tetrasporophytes and gametophytes were estimated after 14 days in culture. Relative growth rates (RGRs) for holdfast areas were 7.08-28.38% day-1 for tetrasporophytes and 11.58-23.67% day-1 for gametophytes. At 35℃, holdfasts of tetrasporophytes survived with RGRs of 7.08-23.28% day-1 but those of gametophytes died. Maximal holdfast growth of tetrasporophytes occurred at 30℃ and 100 µmol photons m-2 s-1, which were different from gametophytes (25℃ and 100 µmol photons m-2 s-1). RGRs of tetrasporophytic sporelings were 2.93-11.11% day-1 and were between 0.78-10.82% day-1 for gametophytes. Maximal growth of A. vermiculophyllum sporelings occurred at 25℃ and 60 µmol photons m-2 s-1 for tetrasporophytes, and at 20℃ and 100 µmol photons m-2 s-1 for gametophytes. In conclusion, the present results indicate that carpospores could be used as resources of spore-seedling methods having genetic diversity for mass field cultivation because tetrasporophytes showed higher-temperature tolerance and faster-growing ability than gametophytes of A. vermiculophyllum in the discoid holdfast and sporeling stages.

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References

  1. Alveal KHCEC, Romo H, Werlinger C, Oliveira EC (1997) Mass cultivation of the agar-producing alga Gracilaria chilensis (Rhodophyta) from spores. Aquaculture 148:77-83 https://doi.org/10.1016/S0044-8486(96)01415-9
  2. Buschmann AH, Correa JA, Westernmeier E, Hernandez-Gonzalez MDC, Normabuena R (2001) Red algal farming in Chile: A review. Aquaculture 194:203-220 https://doi.org/10.1016/S0044-8486(00)00518-4
  3. Glenn EP, Moore D, Fitzsimmons K, Azevedo C (1996) Spore culture of the edible red seaweed, Gracilaria parvispora (Rhodophyta). Aquaculture 142:59-74 https://doi.org/10.1016/0044-8486(95)01249-4
  4. Guillemin ML, Sepulveda RD, Correa JA, Destombe C (2013) Differential ecological responses to environmental stress in the life history phases of the isomorphic red alga Gracilaria chilensis (Rhodophyta). J Appl Phycol 25:215-224 https://doi.org/10.1007/s10811-012-9855-8
  5. Guzman-Uriostegui A, Robledo D (1999) Factors affecting sporulation of Gracilaria cornea (Gracilariales, Rhodophyta) carposporophytes from Yucatan, Mexico. Hydrobiologia 398:285-290 https://doi.org/10.1023/A:1017071427664
  6. Jayasankar R, Varghese S (2002) Cultivation of marine red alga Gracilaria edulis (Gigartinales, Rhodophyta) from spores. Indian J Mar Sci 31:75-77
  7. Kain JM, Destombe C (1995) A review of the life history, reproduction and phenology of Gracilaria. J Appl Phycol 7:269-281 https://doi.org/10.1007/BF00004001
  8. Kim YS, Choi HG, Kim HG, Nam KW, Sohn CH (1998) Reproductive phenology of Gracilaria verrucosa (Rhodophyta) in Cheongsapo near Pusan, Korea. J Fish Sci Tech 1:147-151
  9. Kim YS, Choi HG, Nam KW (2001) Effects of light, desiccation and salinity for the spore discharge of Gracilaria verrucosa (Rhodophyta) in Korea. J Fish Sci Tech 4:257-260
  10. Mantri VA, Thakur MC, Kumar M, Reddy CRK, Jha B (2009) The carpospore culture of industrially important red alga Gracilaria dura (Gracilariales, Rhodophyta). Aquaculture 297:85-90 https://doi.org/10.1016/j.aquaculture.2009.09.004
  11. Michetti KM, Martin LA, Leonardi PI (2013) Carpospore release and sporeling development in Gracilaria gracilis (Gracilariales, Rhodophyta) from the southwestern Atlantic coast (Chubut, Argentina). J Appl Phycol 25:1917-1924. doi:10.1007/s10811-013-0029-0
  12. Orduna-Rojas J, Robledo D (1999) Effects of irradiance and temperature on the release and growth of carpospores from Gracilaria cornea J. Agardh (Gracilariales, Rhodophyta). Bot Mar 42(2):315-319. doi:10.1515/BOT.1999.035
  13. Provasoli L (1968) Media and prospects for the cultivation of marine algae. In: Watanabe A, Hattori A (eds) Proceeding of the US-Japan conference, Japanese Society for Plant Physiology, Tokyo, pp 63-75
  14. Polifrone M, Masi FD, Gargiulo GM (2006) Alternative pathways in the life history of Gracilaria gracilis (Gracilariales, Rhodophyta) from north-eastern Sicily (Italy). Aquaculture 261:1003-1013 https://doi.org/10.1016/j.aquaculture.2006.08.026
  15. Serisawa Y, Yokohama Y, Aruga Y, Tanaka J (2002) Growth of Ecklonia cava (Laminariales, Phaeophyta) sporophytes transplanted to a locality with different temperature conditions. Phycol Res 50:201-207. doi:10.1046/j.1440-1835.2002.00274.x
  16. Shang YC (1976) Economic aspects of Gracilaria culture in Taiwan. Aquaculture 8:1-7 https://doi.org/10.1016/0044-8486(76)90014-4
  17. Sokal RR, Rohlf FJ (1995) Biometry. Freeman, New York, 880 p
  18. Yokoya NS, Kakita H, Obika H, Kitamura T (1999) Effects of environmental factors and plant growth regulators on growth of the red alga Gracilaria vermiculophylla from Shikoku Island, Japan. Hydrobiologia 398:339-347 https://doi.org/10.1023/A:1017072508583
  19. Zemke-White WL, Ohno M (1999) World seaweed utilization: An end of century summary. J Appl Phycol 11:369-376 https://doi.org/10.1023/A:1008197610793