Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Ethanol-producing Thermotolerant Yeast Hanseniaspora opuntiae from Senecio cruentus

시네라리아 꽃으로부터 에탄올 생산성 및 내열성이 우수한 효모 Hanseniaspora opuntiae 균주 분리

  • Yoon, Jeong-Ah (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University) ;
  • Do, Young-Eun (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University) ;
  • Park, Eun-Hee (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University) ;
  • Bae, Young-Woo (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University) ;
  • Kim, Myoung-Dong (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University)
  • 윤정아 (강원대학교 바이오산업공학부) ;
  • 도영은 (강원대학교 바이오산업공학부) ;
  • 박은희 (강원대학교 바이오산업공학부) ;
  • 배영우 (강원대학교 바이오산업공학부) ;
  • 김명동 (강원대학교 바이오산업공학부)
  • Received : 2020.03.09
  • Accepted : 2020.05.27
  • Published : 2020.06.28
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Abstract

The MBY/L6793 strain showing the highest ethanol yield of 0.48 ± 0.00 g ethanol/ g glucose was isolated from Senecio cruentus. Its ethanol yield was approximately 1.5 times that of the MBY/L6986 isolated from Callistephus chinensis. The strain was identified as Hanseniaspora opuntiae by sequence analysis of the 18S rRNA gene, and the sequenced gene was registered to the GenBank (MN859968). When grown at 40℃, the strain produced 3.82 ± 0.98 g ethanol from 20 g glucose and 10.05 ± 0.06 g ethanol from 60 g glucose, corresponding to approximately 2.45 and 5.74 times, respectively, compared to the control strain H. opuntiae KCCM50747. The MBY/L6793 strain was deposited to KCTC (Korean Collection for Type Culture) as KCTC37025.

다양한 꽃으로부터 분리된 160점의 효모 균주 중 시네라리아 꽃에서 분리된 MBY/L6793은 0.48 ± 0.00 g ethanol/g glucose 으로 분리된 균주 중 가장 우수한 에탄올 생산 수율을 나타냈으며, 과꽃으로부터 분리된 MBY/L6986 균주의 약 1.5배 수준이었다. MBY/L6793 균주의 18s rRNA 유전자의 염기서열을 분석한 결과 Hanseniaspora opuntiae로 동정되었으며, 분석된 염기서열은 GenBank (MN859968)에 등록하였다. 40℃에서 배양하였을 때, H. opuntiae MBY/L6793 균주는 20 g의 포도당으로부터 3.82 ± 0.98 g의 에탄올을 생산하였으며, 60g의 포도당으로부터 10.05 ± 0.06 g의 에탄올을 생산하여 대조구 균주인 H. opuntiae KCCM50747 균주의 약 2.45배와 5.74배 수준이었다. H. opuntiae MBY/L6793 균주는 한국생물자원센터에 KCTC37025로 기탁하였다.

Keywords

References

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