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Characterization of Rajath Bhasma and Evaluation of Its Toxicity in Zebrafish Embryos and Its Antimicrobial Activity

  • Kalimuthu, Kalishwaralal (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Kim, Ji Min (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Subburaman, Chandramohan (Department of Biotechnology, Kalasalingam University) ;
  • Kwon, Woo Young (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Hwang, Sung Hyun (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Jeong, Sehan (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Jo, Min Geun (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Kim, Hyung Joo (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Park, Ki Soo (Department of Biological Engineering, College of Engineering, Konkuk University)
  • 투고 : 2019.11.11
  • 심사 : 2020.03.16
  • 발행 : 2020.06.28

초록

In India, nanotechnology has been used in therapeutic applications for several millennia. One example of a traditional nanomedicine is Rajath Bhasma (also called calcined silver ash), which is used as an antimicrobial and for the treatment of various ailments and conditions such as memory loss, eye diseases, and dehydration. In this study, we aimed to characterize the physical composition and morphology of Rajath Bhasma and its suitability for use as a non-toxic antimicrobial agent. First, Rajath Bhasma was physically characterized via i) Fourier-transform infrared spectroscopy to analyze the surface functional groups, ii) scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy to observe the morphology and elemental composition, and iii) X-ray diffraction to determine the crystalline phases. Thereafter, functional characterization was performed through toxicity screening using zebrafish embryos and through antimicrobial activity assessment against gram-positive (Staphylococcus epidermidis) and gram-negative (Escherichia coli) bacteria. Rajath Bhasma was found to harbor alkene, hydroxyl, aldehyde, and amide functional groups originating from biological components on its surface. The main component of Rajath Bhasma is silver, with particle size of 170-210 nm, and existing in the form of spherical aggregates with pure crystalline silver structures. Furthermore, Rajath Bhasma did not exert toxic effects on zebrafish embryos at concentrations below 5 ㎍/ml and exhibited effective antimicrobial activity against both gram-positive and gram-negative bacteria. The present results indicate that Rajath Bhasma is a potentially effective antimicrobial agent without toxicity when used at concentrations below 5 ㎍/ml.

키워드

참고문헌

  1. Kalimuthu Kalishwaralal, Venkataraman Deepak, SureshBabu Ram Kumar Pandian, Muniasamy Kottaisamy, Selvaraj BarathmaniKanth, Bose Kartikeyan, et al. 2010. Biosynthesis of silver and gold nanoparticles using Brevibacterium casei. Colloids Surf. B Biointerfaces 77: 257-262. https://doi.org/10.1016/j.colsurfb.2010.02.007
  2. Royan CU. 1957. Siddha hospital pharmacopeia. Government of Tamil Nadu. 45-47.
  3. Fritts M, Crawford CC, Quibell D, Gupta A, Jonas WB, Coulter I, et al. 2008. Traditional Indian medicine and homeopathy for HIV/AIDS: A review of the literature. AIDS Res. Ther. 5: 25. https://doi.org/10.1186/1742-6405-5-25
  4. Daniel Beaudet, Simona Badilescu, Kiran Kuruvinashetti, Ahmad Sohrabi Kashani, Dilan Jaunky, Sylvie Ouellette, et al. 2017. Comparative study on cellular entry of incinerated ancient gold particles (Swarna Bhasma) and chemically synthesized gold particles. Sci. Rep. 7: 10678. https://doi.org/10.1038/s41598-017-10872-3
  5. Wijnhoven SWP, Peijnenburg WJGM, Herberts CA, Hagens WI, Oomen AG, Heugens EHW, et al. 2009. Nano-silver - a review of available data and knowledge gaps in human and environmental risk assessment. Nanotoxicology 3: 109-138. https://doi.org/10.1080/17435390902725914
  6. Nagarajan S, Krishnaswamy S, Pemiah B, Rajan KS, Krishnan U, Sethuraman S. 2014. Scientific insights in the preparation and characterisation of a lead-based Naga Bhasma. Indian J. Pharm. Sci. 76: 38-45.
  7. Bagul MS, Kanaki NS, Rajani M. 2005. Evaluation of free radical scavenging properties of two classical polyherbal formulations. Indian J. Exp. Biol. 43: 732-736.
  8. Donga SB, Parikh P, Induben U. 2012. PA01.45. An ayurvedic management of vandhyatva w.s.r. to cervical factor. Anc. Sci. Life 32: S95. https://doi.org/10.4103/0257-7941.112094
  9. Mitra A, Chakraborty S, Auddy B, Tripathi P, Sen S, Saha AV, et al. 2002. Evaluation of chemical constituents and free-radical scavenging activity of Swarnabhasma (gold ash), an ayurvedic drug. J. Ethnopharmacol. 80: 147-153. https://doi.org/10.1016/S0378-8741(02)00008-9
  10. Hamouda RA, Hussein MH, Abo-elmagd RA, Bawazir SS. 2019. Synthesis and biological characterization of silver nanoparticles derived from the Cyanobacterium Oscillatoria limnetica. Sci. Rep. 9: 13071. https://doi.org/10.1038/s41598-019-49444-y
  11. AshaRani PV, Mun GLK, Hande MP, Valiyaveettil S. 2009. Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 3: 279-290. https://doi.org/10.1021/nn800596w
  12. Bilberg K, Hovgaard MB, Besenbacher F, Baatrup E. 2012. In vivo toxicity of silver nanoparticles and silver ions in zebrafish (Danio rerio). J. Toxicol. 2012: 293784.
  13. Yoo MH, Rah YC, Choi J, Park S, Park H-C, Oh KH, et al. 2016. Embryotoxicity and hair cell toxicity of silver nanoparticles in zebrafish embryos. Int. J. Pediatr. Otorhinolaryngol. 83: 168-174. https://doi.org/10.1016/j.ijporl.2016.02.013
  14. Liu X, Dumitrescu E, Kumar A, Austin D, Goia D, Wallace K, et al. 2019. Differential lethal and sublethal effects in embryonic zebrafish exposed to different sizes of silver nanoparticles. Environ. Pollut. 248: 627-634. https://doi.org/10.1016/j.envpol.2019.02.085
  15. Kalishwaralal K, Jeyabharathi S, Sundar K, Muthukumaran A. 2016. A novel one-pot green synthesis of selenium nanoparticles and evaluation of its toxicity in zebrafish embryos. Artif. Cells Nanomed. Biotechnol. 44: 471-477. https://doi.org/10.3109/21691401.2014.962744
  16. Kalishwaralal K, Deepak V, Ramkumarpandian S, Nellaiah H, Sangiliyandi G. 2008. Extracellular biosynthesis of silver nanoparticles by the culture supernatant of Bacillus licheniformis. Mater. Lett. 62: 4411-4413. https://doi.org/10.1016/j.matlet.2008.06.051
  17. Maria E Diaz-Casado, Iryna Rusanova, Paula Aranda, Marisol Fernandez-Ortiz, Ramy K A Sayed, Beatriz I Fernandez-Gil, et al. 2018. In vivo determination of mitochondrial respiration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated zebrafish reveals the efficacy of melatonin in restoring mitochondrial normalcy. Zebrafish 15: 15-26. https://doi.org/10.1089/zeb.2017.1479
  18. Kalimuthu Kalishwaralal, Selvaraj BarathManiKanth, Sureshbabu Ram Kumar Pandian, Venkataraman Deepak, Sangiliyandi Gurunathan. 2010. Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloids Surf. B Biointerfaces 79: 340-344. https://doi.org/10.1016/j.colsurfb.2010.04.014
  19. Chaturvedi R,Jha C. 2011. Standard manufacturing procedure of Rajata Bhasma. Ayu 32: 566-571. https://doi.org/10.4103/0974-8520.96135
  20. Peng Wu, Wei Li, Qiong Wu, Yushan Liu, Shouxin Liu . 2017. Hydrothermal synthesis of nitrogen-doped carbon quantum dots from microcrystalline cellulose for the detection of $Fe^{3+}$ ions in an acidic environment. RSC Adv. 7: 44144-44153. https://doi.org/10.1039/C7RA08400E
  21. Mahadevan S, Vijayakumar S, Arulmozhi, P. 2017. Green synthesis of silver nano particles from Atalantia monophylla (L) Correa leaf extract, their antimicrobial activity and sensing capability of $H_2O_2$. Microb. Pathog. 113: 445-450. https://doi.org/10.1016/j.micpath.2017.11.029
  22. Nakason K, Panyapinyopol B, Kanokkantapong V, Virya-empikul N, Kraithong W, Pavasant P. 2018. Hydrothermal carbonization of unwanted biomass materials: Effect of process temperature and retention time on hydrochar and liquid fraction. J. Energy Inst. 91: 786-796. https://doi.org/10.1016/j.joei.2017.05.002
  23. Baia TC, Gama RA, Silva De Lima LA, Lima KMG. 2016. FTIR microspectroscopy coupled with variable selection methods for the identification of flunitrazepam in necrophagous flies. Anal. Methods 8: 968-972. https://doi.org/10.1039/C5AY02342D
  24. Bharani M, Thirunethiran Karpagam, Varalakshmi B, Indria S. 2012. Synthesis and characterization of silver nano particles from wrightia tinctoria. Int. J. Appl. Biol. Pharm. Tech. 3: 58-63.
  25. Wojnarowicz J, Opalinska A, Chudoba T, Grerlotka S, Mukhovskyi, Pierzylowska, et al. 2016. Effect of water content in ethylene glycol solvent on the size of ZnO nanoparticles prepared using microwave solvothermal synthesis. J. Nanomater. 2016: ID2789871.
  26. Hiremath R, Jha CB, Narang KK. 2010. Vanga Bhasma and its XRD analysis. Ancient. Sci. Life. 29: 24-28.