Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory effects of a mixture of coffee and sword bean extracts

커피와 작두콩 추출물의 혼합에 따른 항염증 효과

  • Bae, Hun Cheon (Department of Food Science & Technology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Park, Jung Up (College of Pharmacy and Research Institute of Drug Development, Chonnam National University) ;
  • Moon, Jae-Hak (Department of Food Science & Technology, College of Agriculture and Life Sciences, Chonnam National University)
  • 배훈천 (전남대학교 농업생명과학대학 식품공학과) ;
  • 박정업 (전남대학교 약학대학) ;
  • 문제학 (전남대학교 농업생명과학대학 식품공학과)
  • Received : 2020.02.21
  • Accepted : 2020.04.13
  • Published : 2020.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Coffee is one of the most widely consumed beverages in the world, and sword bean (Canavalia gladiata, SB) reportedly possesses various biological activities. Therefore, in this study, to reduce caffeine intake and improve coffee function, SB was selected as a supplementary material for blending coffee. The antioxidant and anti-inflammatory activities of coffee with the SB extract at concentrations of 0.1-0.5% (v/v) were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and RAW 264.7 cells, respectively. The DPPH radical-scavenging activity of SB-treated coffee depended on the concentration of the SB extract. In the cell culture experiment, cytotoxicity was not observed at any SB concentration. In addition, the inducible nitric oxide synthesis protein expression as well as the increases in nitric oxide and interleukin-6 expression were effectively inhibited by SB addition to the coffee. These results indicate that SB might be useful as a supplementary ingredient to enhance the caffeinated drink functions.

커피에 작두콩 추출물의 혼합에 따른 항염증 효과를 평가하였다. 커피 추출물과 작두콩 추출물을 1:1, 1:2, 1:3, 1:4, 1:5 (v/v)의 비율로 혼합한 시료를 대상으로 DPPH를 이용한 항산화 활성평가와 세포독성 평가 및 LPS로 염증이 유도된 RAW 264.7 세포에서 염증 매개물질인 NO, 염증성 cytokine인 TNF-α와 IL-6의 생성 및 iNOS 단백질의 발현을 측정하였다. 그 결과, DPPH radical-scavenging 활성은 작두콩 추출물의 농도에 의존적으로 높게 나타났으며, 커피와 작두콩 1:4 (v/v)의 혼합 비율에서 대략 80%의 DPPH radical-scavenging 활성을 보였다. MTS assay로 세포 생존율을 분석한 결과, 커피와 작두콩 1:1, 1:2, 1:3, 1:4, 1:5(v/v) 혼합 추출물 모두에서 세포독성이 나타나지 않았다. LPS로 염증이 유도된 RAW 264.7 세포에서 TNF-α의 발현 저해 효과는 관찰되지 않았지만, iNOS 단백질 발현과 그에 따른 NO와 IL-6의 생성은 효과적으로 억제됨이 확인되었다. 특히, 커피와 작두콩 1:2 (v/v) 혼합 비율에서 IL-6와 iNOS의 발현이 유의하게 감소하였다. 이와 같은 결과를 종합하여볼 때, 작두콩을 커피의 블렌딩 소재로 이용함으로써 항산화와 항염증 기능이 향상된 커피음료의 제조에 응용할 수 있을 것으로 시사되었다.

Keywords

References

  1. Bystrom LM, Guzman ML, Rivella S. Iron and reactive sxygen species: Friends or toes of cancer cells? Antioxid. Redox Signaling. 20: 1917-1924 (2014) https://doi.org/10.1089/ars.2012.5014
  2. Chang MI, Kim JY, Kim SJ, Baek SH. Effect of sword bean Chunggukjang addition on quality of Kochujang. J. Korean Soc. Food Sci. Nutr. 40: 1292-1299 (2011) https://doi.org/10.3746/jkfn.2011.40.9.1292
  3. Cho YS, Bae YI, Shim KH. Chemical components in different parts of Korean sword bean (Canavalia gladiata). Korean J. Postharvest Sci. Technol. 6: 475-480 (1999)
  4. European Food Safety Authority. Panel on Dietetic Products, Nutrition and Allergies (NDA). Scientific opinion on the safety of caffeine. EFSA J. 13: 4102-4107 (2015)
  5. Ekanayake S, Skog K, Asp NG. Canavanine content in sword bean (Canavalia gladiata): Analysis and effect of processing. Food Chem. Toxicol. 45: 797-803 (2007) https://doi.org/10.1016/j.fct.2006.10.030
  6. Green SJ, Scheller LF, Marletta MA, Seguin MC, Klotz FW, Slayter M, Nelson BJ, Nacy CA. Nitric oxide: cytokine-regulation of nitric oxide in host resistance to intracellular pathogens. Immunol. Lett. 43: 87-94 (1994) https://doi.org/10.1016/0165-2478(94)00158-8
  7. Jeon KS, Na HJ, Kim YM, Kwon HJ. Antiangiogenic activity of 4-O-methylgallic acid from Canavalia gladiata, dietary legume. Biochem. Biophys. Res. Commun. 330(4): 1268-1274 (2005) https://doi.org/10.1016/j.bbrc.2005.03.109
  8. Kim JP, Lee HH, Moon JH, Ha DR, Kim ES, K JH, Seo KW. Isolation and Identification of antioxidants from methanol extract of sword bean (Canavalia gladiata). Korean J. Food Sci. Technol. 45: 777-784 (2013) https://doi.org/10.9721/KJFST.2013.45.6.777
  9. Korea Customs Service. Trade statistics. Available from: https://unipass.customs.go.kr/ets/index.do. Accessed Nov. 23, 2019.
  10. Korean Statistical Information Service. Ministry of Food and Drug Safety. Production of food manufacturing and processing establishments. Available from: http://kosis.kr/index/index.do. Accessed Nov. 23, 2019.
  11. Lafranconi A, Micek A, De Paoli P, Bimonte S, Rossi P, Quagliariello V, Berretta M. Coffee intake decreases risk of ostmenopausal breast cancer: A dose-response meta-analysis on rospective cohort studies. Nutrients 10: 112 (2018) https://doi.org/10.3390/nu10020112
  12. Loftfield E, Rothwell JA, Sinha R, Keski-Rahkonen P, Robinot N, Albanes D, Weinstein SJ, Derkach A, Sampson J, Scalbert A, Freedman ND. Prospective investigation of serum metabolites, coffee drinking, liver cancer incidence, and liver disease mortality. JNCI, J. Natl. Cancer Inst. 112: 286-294 (2020) https://doi.org/10.1093/jnci/djz122
  13. Loomis D, Guyton KZ, Grosse Y, Lauby-Secretan B, El Ghissassi F, Bouvard V, et al. Carcinogenicity of drinking coffee, matmate, and very hot beverages. Lancet Oncol. 17(7): 877-878 (2016) https://doi.org/10.1016/S1470-2045(16)30239-X
  14. Lopez-Garcia E, Guallar-Castillon P, Leon-Munoz L, Graciani A, Rodriguez-Artalejo F. Coffee consumption and health-related quality of life. Clin. Nutr. 33: 143-149 (2014) https://doi.org/10.1016/j.clnu.2013.04.004
  15. Lopez-Garcia E, Rodriguez-Artalejo F, Rexrode KM, Logroscino G, Hu, FB, van Dam RM. Coffee consumption and risk of stroke in women. Circulation. 119: 1116-1123 (2009) https://doi.org/10.1161/CIRCULATIONAHA.108.826164
  16. Mungrue IN, Husain M, Stewart DJ. The role of NOS in heart failure: lessons from murine genetic models. Heart Failure Rev. 7: 407-422 (2002) https://doi.org/10.1023/A:1020762401408
  17. Nakatsuka Y, Nagasawa T, Yumoto Y, Nakazawa F, Furuichi Y. Inhibitory effects of sword bean extract on alveolar bone resorption induced in rats by Porphyromonas gingivalis infection. J. Periodontal Res. 49: 801-809 (2014) https://doi.org/10.1111/jre.12166
  18. Negi AS, Darokar MP, Chattopadhyay SK, Garg A, Bhattacharya AK, Srivastava V, Khanuja PS. Synthesis of a growth promoter from gallic acid. Bioorg. Med. Chem. Lett. 15: 1243-1247 (2005) https://doi.org/10.1016/j.bmcl.2004.11.079
  19. Nieber K. The impact of coffee on health. Planta Med. 83: 1256-1263 (2017) https://doi.org/10.1055/s-0043-115007
  20. Nimenibo-Uadia R. Effect of aqueous extract of Canavalia ensiformis seeds on hyperlipidaemia and hyperketonaemia in alloxaninduced diabetic rats. Biokemistri 15: 7-15 (2003)
  21. Pandurangan AK, Mohebali N, Norhaizan ME, Looi CY. Gallic acid attenuates dextran sulfate sodium-induced experimental colitis in BALB/c mice. Drug Des., Dev. Ther. 9: 3923-934 (2015)
  22. Park JU, Kim SJ, Na CS, Choi CH, Seo CS, Son JK, Kang BY, Kim YR. Chondroprotective and anti-inflammatory effects of ChondroT, a new complex herbal medication. BMC Complementary Altern. Med. 16: 1-11 (2016)
  23. Tamura S, Takahashi N, Murofushi N, Yokota T, Kato J, Shiotani Y. Isolation of two new gibberellins from immature seeds of Canavalia. Planta 75: 279-282 (1967) https://doi.org/10.1007/BF00386327
  24. Wootton-Beard PC, Ryan L. Improving public health?: The role of antioxidant-rich fruit and vegetable beverages. Food Res. Int. 44: 3135-3148 (2011) https://doi.org/10.1016/j.foodres.2011.09.015