Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Conjugated linoleic acid producing potential of lactobacilli isolated from goat (AXB) rumen fluid samples

  • Tyagi, Amrish Kumar (Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute) ;
  • Kumar, Sachin (Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute) ;
  • Choudhury, Prasanta Kumar (Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute) ;
  • Tyagi, Bhawna (Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute) ;
  • Tyagi, Nitin (Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute)
  • Received : 2019.01.23
  • Accepted : 2019.07.26
  • Published : 2020.08.01
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Abstract

Objective: The present investigation was aimed to explore the potential of lactobacilli for conjugated linoleic acid (CLA) production, isolated from rumen fluid samples of lactating goats. Methods: A total of 64 isolates of lactobacilli were obtained using deMan-Rogosa-Sharpe (MRS) agar from rumen fluid of goats and further subjected to morphological and biochemical characterizations. Isolates found as gram-positive, catalase negative rods were presumptively identified as Lactobacillus species and further confirmed by genus specific polymerase chain reaction (PCR). The phylogenetic tree was constructed from the nucleotide sequences using MEGA6. Results: Out of the 64 isolates, 23 isolates were observed positive for CLA production by linoleate isomerase gene-based amplification and quantitatively by UV-spectrophotometric assay for the conversion of linoleic acid to CLA as well as gas chromatography-based assay. In all Lactobacillus species cis9, trans11 isomer was observed as the most predominant CLA isomer. These positive isolates were identified by 16S rRNA gene-based PCR sequencing and identified to be different species of L. ingluviei (2), L.salivarius (2), L. curvatus (15), and L. sakei (4). Conclusion: The findings of the present study concluded that lactic acid bacteria isolated from ruminal fluid samples of goat have the potential to produce bioactive CLA and may be applied as a direct fed microbial to enhance the nutraceutical value of animal food products.

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References

  1. German JB, Gibson RA, Krauss RM, et al. A reappraisal of the impact of dairy foods and milk fat on cardiovascular disease risk. Eur J Nutr 2009;48:191-203. https://doi.org/10.1007/s00394-009-0002-5
  2. Kim L, Park Y, Park, Y. trans-10,cis-12 CLA promotes osteoblastogenesis via SMAD mediated mechanism in bone marrow mesenchymal stem cells. J Funct Foods 2014;8:367-76. https://doi.org/10.1016/j.jff.2014.04.006
  3. Yang B, Chen H, Gu Z, et al. Synthesis of conjugated linoleic acid by the linoleate isomerase complex in food-derived lactobacilli. J Appl Microbiol 2014;117:430-9. https://doi.org/10.1111/jam.12524
  4. Yang B, Chen H, Stanton C, et al. Review of the roles of conjugated linoleic acid in health and disease. J Funct Food 2015;15:314-25. https://doi.org/10.1016/j.jff.2015.03.050
  5. Kepler CR, Hirons KP, McNeill JJ, Tove SB. Intermediates and products of the biohydrogenation of linoleic acid by Butyrinvibrio Fibrisolvens. J Biol Chem 1966;241:1350-4. https://doi.org/10.1016/S0021-9258(18)96781-5
  6. Corl BA, Baumgard LH, Dwyer DA, Griinari JM, Phillips BS, Bauman DE. The role of ${\Delta}9$-desaturase in the production of cis-9, trans-11 CLA. J Nutr Biochem 2001;12:622-30. https://doi.org/10.1016/S0955-2863(01)00180-2
  7. Benjamin S, Prakasan P, Sreedharan S, Wright AD, Spener F. Pros and cons of CLA consumption: an insight from clinical evidences. Nutr Metab 2015;12:4. https://doi.org/10.1186/1743-7075-12-4
  8. Macdonald HB. Conjugated linoleic acid and disease prevention: a review of current knowledge. J Am Coll Nutr 2000;19:111S-8S. https://doi.org/10.1080/07315724.2000.10718082
  9. Sosa-Castaneda J, Hernandez-Mendoza A, Astiazaran-Garcia H, et al. Screening of Lactobacillus strains for their ability to produce conjugated linoleic acid in milk and to adhere to the intestinal tract. J Dairy Sci 2015;98:6651-9. https://doi.org/10.3168/jds.2014-8515
  10. Griinari JM, Bauman DE. Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In: Yurawecz MP, Mossoba MM, Kramer JKG, Pariza MW, Nelson GJ, editors. Advances in conjugated linoleic acid research. Champaign, IL, USA: AOCS Press; 1999. pp. 180-201.
  11. Puniya AK, Chaitanya S, Tyagi AK, De S, Singh K. Conjugated linoleic acid producing potential of lactobacilli isolated from the rumen of cattle. J Ind Microbiol Biotechnol 2008;35:1223-8. https://doi.org/10.1007/s10295-008-0429-3
  12. Asraf Hussain SK, Srivastava A, Tyagi A, et al. Characterization of CLA-producing Butyrivibrio spp. reveals strain-specific variations. 3 Biotech 2016;6:90. https://doi.org/10.1007/s13205- 016-0401-2
  13. Dahiya DK, Puniya AK. Isolation, molecular characterization and screening of indigenous lactobacilli for their abilities to produce bioactive conjugated linoleic acid (CLA). J Food Sci Technol 2017;54:792-801. https://doi.org/10.1007/s13197-017-2523-x
  14. Jena R, Choudhury PK, Puniya AK, Tomar SK. Isolation and species delineation of genus Bifidobacterium using PCR-RFLP of partial hsp60 gene fragment. LWT-Food Sci Technol 2017;80:286-93. https://doi.org/10.1016/j.lwt.2017.02.032
  15. Dubernet S, Desmasures N, GueguenM. A PCR-based method for identification of lactobacilli at the genus level. FEMS Microbiol Lett 2002;214:271-5. https://doi.org/10.1111/j. 1574-6968.2002.tb11358.x
  16. Gorissen L, Weckx S, Vlaeminck B, et al. Linoleate isomerase activity occurs in lactic acid bacteria strains and is affected by pH and temperature. J Appl Microbiol 2011;111:593-606. https://doi.org/10.1111/j.1365-2672.2011.05087.x
  17. Barrett E, Ross R, Fitzgerald G, Stanton C. Rapid screening method for analyzing the conjugated linoleic acid production capabilities of bacterial cultures. Appl Environ Microbiol 2007;73:2333-7. http://doi.org/10.1128/AEM.01855-06
  18. O'Fallon JV, Busboom JR, Nelson ML, Gaskins CT. A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs. J Anim Sci 2007;85:1511-21. https://doi.org/10.2527/jas.2006-491
  19. Lane DJ. 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M, editors. Nucleic acid techniques inbacterial systematics. New York, NY, USA: John Wiley; 1991. pp. 115-76.
  20. Ogawa J, Matsumura K, Kishino S, Omura Y, Shimizu S. Conjugated linoleic acid accumulation via 10-hydroxy-12-octadecaenoic acid during microaerobic transformation of linoleic acid by Lactobacillus acidophilus. Appl Environ Microbiol 2001;67:1246-52. https://doi.org/10.1128/AEM.67.3.1246- 1252.2001
  21. Khosravi A, Safari M, Khodaiyan F, Gharibzahedi SMT. Bioconversion enhancement of conjugated linoleic acid by Lactobacillus plantarum using the culture media manipulation and numerical optimization. J Food Sci Technol 2015;52:5781-9. https://doi.org/10.1007/s13197-014-1699-6
  22. Kishino S, Ogawa J, Omura Y, Matsumura K, Shimizu S. Conjugated linoleic acid production from linoleic acid by lactic acid bacteria. J Am Oil Chem Soc 2002;79:159-63. https://doi.org/10.1007/s11746-002-0451-4
  23. Andrade JC, Ascencao K, Gullon P, et al. Production of conjugated linoleic acid by food-grade bacteria: a review. Int J Dairy Technol 2012;65:467-81. https://doi.org/10.1111/j.1471-0307. 2012.00871.x
  24. Li H, Liu Y, Liu X, Zhang H. Conjugated linoleic acid conversion by six Lactobacillus plantarum strains cultured in MRS broth supplemented with sunflower oil and soymilk. J Food Sci 2012;77:M330-6. https://doi.org/10.1111/j.1750-3841. 2012.02723.x
  25. Lee K, Paek K, Lee H, Park JH, Lee Y. Antiobesity effect of trans-10, cis-12-conjugated linoleic acid-producing Lactobacillus plantarum PL62 on diet-induced obese mice. J Appl Microbiol 2007;103:1140-6. https://doi.org/10.1111/j.1365- 2672.2007.03336.x
  26. Lin TY, Lin CW, Wang YJ. Production of conjugated linoleic acid by enzyme extract of Lactobacillus acidophilus CCRC 14079. Food Chem 2003;83:27-31. https://doi.org/10.1016/S0308-8146(03)00032-3
  27. Ando A, Ogawa J, Kishino S, Shimizu S. CLA production from ricinoleic acid by lactic acid bacteria. J Am Oil Chem Soc 2003;80:889-94. https://doi.org/10.1007/s11746-003-0790-1
  28. Farmani J, Safari M, Roohvand F, Razavi SH, Aghasadeghi MR, Noorbazargan H. Conjugated linoleic acid-producing enzymes: a bioinformatics study. Eur J Lipid Sci Technol 2010;112:1088-100. https://doi.org/10.1002/ejlt.201000360
  29. Koort J, Vandamme P, Schillinger U, Holzapfel W, Bjorkroth J. Lactobacillus curvatus subsp. melibiosus is a later synonym of Lactobacillus sakei subsp. carnosus. Int J Syst Evol Microbiol 2004;54:1621-6. https://doi.org/10.1099/ijs.0.63164-0
  30. Lee J, Jang J, Kim B, Kim J, Jeong G, Han H. Identification of Lactobacillus sakei and Lactobacillus curvatus by multiplex PCR-based restriction enzyme analysis. J Microbiol Methods 2004;59:1-6. https://doi.org/10.1016/j.mimet.2004.05.004
  31. Klein G, Dicks LMT, Pack A, et al. Emended descriptions of Lactobacillus sake (Katagiri, Kitahara, and Fukami) and Lactobacillus curvatus (Abo-Elnaga and Kandler): numerical classification revealed by protein fingerprinting and identification based on biochemical patterns and DNA-DNA hybridizations. Int J Syst Bacteriol 1996;46:367-76. https://doi.org/10.1099/00207713-46-2-367
  32. Gorissen L, Leroy F, De Vuyst L, De Smet S, Raes K. Bacterial production of conjugated linoleic and linolenic acid in foods: a technological challenge. Crit Rev Food Sci Nutr 2015;55:1561-74. https://doi.org/10.1080/10408398.2012.706243
  33. Seo JK, Kim SW, Kim MH, Upadhaya SD, Kam DK, Ha JK. Direct-fed microbials for ruminant animals. Asian-Australas J Anim Sci 2010;23:1657-67. https://doi.org/10.5713/ajas. 2010.r.08
  34. Frizzo LS, Sotto LP, Zbrun MV, et al. Lactic acid bacteria to improve growth performance in young calves fed milk replacer and spray-dried whey powder. Anim Feed Sci Technol 2010;157:159-67. https://doi.org/10.1016/j.anifeedsci.2010.03.005
  35. Ting YS, Saad WZ, Chin SC, Wan HY. Characterization of conjugated linoleic acid-producing lactic acid bacteria as potential probiotic for chicken. Malays J Microbiol 2016;12:15-23. https://dx.doi.org/10.21161/mjm.67214
  36. Mandal A, Paul T, Roy S, et al. Effect of newly isolated Lactobacillus ingluviei ADK10, from chicken intestinal tract on acetaminophen induced oxidative stress in Wistar rats. Indian J Exp Biol 2013;51:174-80.

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