Clinical and Experimental Reproductive Medicine

  • 제47권2호
  • /
  • Pages.85-93
  • /
  • 2020
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Advantages of the outgrowth model for evaluating the implantation competence of blastocysts

  • Kim, Jihyun (Clinical Medicine Division, Korea Institute of Oriental Medicine) ;
  • Lee, Jaewang (Department of Biomedical Laboratory Science, College of Health Science, Eulji University) ;
  • Jun, Jin Hyun (Department of Biomedical Laboratory Science, College of Health Science, Eulji University)
  • 투고 : 2019.08.01
  • 심사 : 2020.01.31
  • 발행 : 2020.06.30
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초록

The implantation process is highly complex and difficult to mimic in vitro, and a reliable experimental model of implantation has yet to be established. Many researchers have used embryo transfer (ET) to assess implantation potential; however, ET with pseudopregnant mice requires expert surgical skills and numerous sacrificial animals. To overcome those economic and ethical problems, several researchers have tried to use outgrowth models to evaluate the implantation potential of embryos. Many previous studies, as well as our experiments, have found significant correlations between blastocyst outgrowth in vitro and implantation in utero by ET. This review proposes the blastocyst outgrowth model as a possible alternative to animal experimentation involving ET in utero. In particular, the outgrowth model might be a cost- and time-effective alternative method to ET for evaluating the effectiveness of culture conditions or treatments. An advanced outgrowth model and further culture of outgrowth embryos could provide a subtle research model of peri- and postimplantation development, excluding maternal effects, and thereby could facilitate progress in assisted reproductive technologies. Recently, we found that outgrowth embryos secreted extracellular vesicles containing specific microRNAs. The function of microRNAs from outgrowth embryos should be elucidated in further researches.

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참고문헌

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