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Interleukin-10-Producing B Cells Help Suppress Ovariectomy-Mediated Osteoporosis

  • Yuhua Wang (Department of Prosthodontics, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology) ;
  • Wei Zhang (Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University) ;
  • Seong-Min Lim (Department of Medical Biotechnology, Yeungnam University) ;
  • Li Xu (Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University) ;
  • Jun-O Jin (Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University)
  • Received : 2020.08.30
  • Accepted : 2020.10.21
  • Published : 2020.12.31
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Abstract

Osteoporosis is prevalent in elderly women and it may cause dental implant failure. In particular, estrogen deficiency in postmenopausal women leads to higher rates of osteoporosis prevalence. Immune cell-mediated effects involving the development of osteoporosis have been studied previously; however, the role of IL-10-producing regulatory B (B10) cells in osteoporosis is largely unclear. Here, we examined the role of B10 cells in osteoporosis. C57BL/6 mice were subjected to ovariectomy (OVX). Fifteen weeks after OVX surgery, the first molar of the right maxillary was extracted, and twenty-four weeks after OVX surgery, serous progression of osteoporosis was observed in the alveolar bone. Moreover, the proportion of CD19+CD5+CD1dhigh regulatory B cells, B10, and CD4+CD25+FoxP3+ regulatory T cells from the spleen of OVX mice decreased during the progression of osteoporosis, compared to controls. In contrast to regulatory cells, IL-17-producing Th (Th17) cell levels were increased in OVX mice. Adoptive transfer of B10 cells to OVX mice led to a decrease in Th17 cell abundance and inhibited the development of osteoporosis in the alveolar bone from OVX mice. Thus, our results suggest that B10 cells may help suppress osteoporosis development.

Keywords

Acknowledgement

We thank the Shanghai Public Health Clinical Center animal facility for maintaining the animals used in this study. This study was supported by the National Research Foundation of Korea (NRF-2019R1C1C1003334 and NRF-2020R1A6A1A03044512) and the National Natural Science Foundation of China (81874164). Yuhua Wang was supported by the Shanghai Natural Science Foundation (20ZR1431900).

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