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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Human transcription factor YY1 could upregulate the HIV-1 gene expression

  • Yu, Kyung Lee (National Research Laboratory of Molecular Virology, Department of Pathology, The Catholic University of Korea) ;
  • Jung, Yu Mi (National Research Laboratory of Molecular Virology, Department of Pathology, The Catholic University of Korea) ;
  • Park, Seong Hyun (National Research Laboratory of Molecular Virology, Department of Pathology, The Catholic University of Korea) ;
  • Lee, Seong Deok (National Research Laboratory of Molecular Virology, Department of Pathology, The Catholic University of Korea) ;
  • You, Ji Chang (National Research Laboratory of Molecular Virology, Department of Pathology, The Catholic University of Korea)
  • Received : 2019.08.27
  • Accepted : 2019.10.11
  • Published : 2020.05.31
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Abstract

Gene expression in HIV-1 is regulated by the promoters in 5' long-terminal repeat (LTR) element, which contain multiple DNA regulatory elements that serve as binding sites for cellular transcription factors. YY1 could repress HIV-1 gene expression and latent infection. Here, however, we observed that virus production can be increased by YY1 over-expression and decreased under YY1 depleted condition by siRNA treatment. To identify functional domain(s) of YY1 activation, we constructed a number of YY1 truncated mutants. Our data show that full-length YY1 enhances the viral transcription both through U3 and U3RU5 promoters. Moreover, the C-terminal region (296-414 residues) of YY1 is responsible for the transcriptional upregulation, which could be enhanced further in the presence of the viral Tat protein. The central domain of YY1 (155-295 residues) does not affect LTR activity but has a negative effect on HIV-1 gene expression. Taken together, our study shows that YY1 could act as a transcriptional activator in HIV-1 replication, at least in the early stages of infection.

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References

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