Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Influence of various concentrations of aflatoxin B1 on in vitro rumen fermentation of a buffalo diet

  • Singh, Ram (Buffalo Nutrition Division, ICAR-Central Institute for Research on Buffaloes) ;
  • Park, Sungkwon (Department of Food Science and Biotechnology, College of Life Science, Sejong University) ;
  • Koo, Jin Su (Department of Food Science and Biotechnology, College of Life Science, Sejong University) ;
  • Balasubramanian, Balamuralikrishnan (Department of Food Science and Biotechnology, College of Life Science, Sejong University)
  • Received : 2020.01.08
  • Accepted : 2020.02.21
  • Published : 2020.03.01
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Abstract

The aim of this study was to evaluate the effect of aflatoxin B1 (AFB1) on in vitro rumen fermentation at various dose levels of 0 (T1), 100 (T2), 200 (T3), and 300 (T4) ppb in a wheat straw-based buffalo diet. The results show that the truly degradable dry matter, truly degradable organic matter, gas production, microbial biomass production and partitioning factor values in the control group (T1) were higher (p < 0.05) than those of the T2, T3, and T4 groups. The total volatile fatty acids, acetate, propionate, and butyrate values in the control group (T1) were higher (p < 0.05) than those of the T2, T3, and T4 groups. The partitioning factor value in the control group (T1) was higher (p < 0.05) than those of the T2, T3, and T4 groups. The partitioning factor values of the T2 and T3 groups were higher (p < 0.05) than that of the T4 group. There was no significant variation in the partitioning factor value between the T2 and T3 group. The acetate : propionate (A : P) ratio in the control group (T1) was lower (p < 0.05) than those of the T2, T3, and T4 groups. The A : P ratio in the T2 group was lower (p < 0.05) than those of the T3 and T4 groups. It was concluded that different levels of AFB1 contamination in feed significantly affect the in vitro rumen fermentation characteristics. Thus, these findings could help to determine the influences of AFB1 in a wheat straw-based buffalo diet. Additionally, it is necessary to manage AFB1 contamination in ruminants.

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References

  1. AFRC (Agricultural and Food Research Council). 1991. Voluntary intake of cattle (Report 8). Nutrition Abstracts and Reviews 61:816-823.
  2. Bhat R, Rai RV, Karim A. 2010. Mycotoxins in food and feed: present status and future concerns. Comprehensive Reviews in Food Science and Food Safety 9:57-81. https://doi.org/10.1111/j.1541-4337.2009.00094.x
  3. Blummel M, Makkar HPS, Becker K. 1997. In vitro gas production: A technique revisited. Journal of Animal Physiology and Animal Nutrition 77:24-34. https://doi.org/10.1111/j.1439-0396.1997.tb00734.x
  4. Cook WO, Richard JL, Osweiller GD, Trampel DW. 1986. Clinical and pathologic changes in acute bovine aflatoxicosis: Rumen motility and tissue and fluid concentrations of aflatoxins B1 and M1. American Journal of Veterinary Research 47:1817-1825.
  5. Cottyn BG, Boucque CV. 1968. Rapid method for the gas chromatographic determination of volatile fatty acids in rumen fluid. Journal of Agricultural and Food Chemistry 16:105-107. https://doi.org/10.1021/jf60155a002
  6. Eaton DL, Gallagher EP. 1994. Mechanisms of aflatoxin carcinogenesis. Annual Review of Pharmacology and Toxicology 34:135-172. https://doi.org/10.1146/annurev.pa.34.040194.001031
  7. Edrington TS, Harvey RB, Kubena LF. 1994. Effect of aflatoxin in growing lambs fed ruminally degradable or escape protein sources. Journal of Animal Science 72:1274-1281. https://doi.org/10.2527/1994.7251274x
  8. Fehr PM, Delage J. 1970. Effect of aflatoxin on fermentation in the rumen. Comptes rendus de l'Academie des Sciences Paris (Series D) 270:550-553.
  9. Fink-Gremmels J. 2008. Mycotoxins in cattle feeds and carry-over to dairy milk. Food Additives and Contaminants 25:172-180. https://doi.org/10.1080/02652030701823142
  10. Gallo A, Giuberti G, Frisvald JC, Bertuzzi T, Nielsen KF. 2015. Review on mycotoxin issues in ruminants: Occurrence in forages, effects of mycotoxin ingestion on health status and animal performance and practical strategies to counteract their negative effects. Toxins 7:3057-3111. https://doi.org/10.3390/toxins7083057
  11. Groopman JD, Cain LG, Kensler TW, Harris CC. 1988. Aflatoxin exposure in human populations: Measurements and relationship to cancer. CRC Critical Review in Toxicology 19:113-145. https://doi.org/10.3109/10408448809014902
  12. Harikrishna CH, Mahender M, Ramana Reddy Y, Gnana Prakash M, Sudhakar K, Pavani M. 2012. Evaluation of in vitro gas production and nutrient digestibility of complete diets supplemented with different levels of thermotolerant yeast in Nellore rams. Veterinary World 5:477-485. https://doi.org/10.5455/vetworld.2012.477-485
  13. Helferich WG, Baldwin RL, Hsieh DPH. 1986b. Aflatoxin B1 metabolism in lactating goats and rats. Journal of Animal Science 62:697-705. https://doi.org/10.2527/jas1986.623697x
  14. Helferich WG, Garrett WN, Hsieh DPH, Baldwin RL. 1986a. Feedlot performance and tissue residues of cattle consuming diets containing aflatoxins. Journal of Animal Science 62:691-696. https://doi.org/10.2527/jas1986.623691x
  15. IARC (International Agency for Research on Cancer). 1993. IARC monographs on the evaluation of carcinogenic risks to humans. Vol. 56. Some naturally occurring substances: Food items and constitution, heterocyclic aromatic amines and mycotoxins. IARC, Geneva, Swiss.
  16. Jiang YH, Yang HJ, Lund P. 2012. Effect of aflatoxin B1 on in vitro ruminal fermentation of rations high in alfalfa hay or ryegrass hay. Animal Feed Science and Technology 175:85-89. https://doi.org/10.1016/j.anifeedsci.2012.03.021
  17. Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science 93:217-222. https://doi.org/10.1017/S0021859600086305
  18. Menke KH, Steingass H. 1988. Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Animal Research Development 28:7-55.
  19. Mojtahedi M, Danesh Mesgaran M, Vakili SA, Hayati-Ashtiani M. 2013. Effect of aflatoxin B1 on in vitro rumen microbial fermentation responses using batch culture. Annual Review and Research in Biology 3:686-693.
  20. Morgavi DP, Riley RT. 2007. An historical overview of field disease outbreaks known or suspected to be caused by consumption of feeds contaminated with Fusarium toxins. Animal Feed Science and Technology 137:201-212. https://doi.org/10.1016/j.anifeedsci.2007.06.002
  21. Pettersson H. 2004. Controlling mycotoxins in animal feed. In: Mycotoxins in food, detection and control (Magan N and Olsen M, eds.), pp. 262-304. Woodhead Publishing Limited, Cambridge, UK.
  22. Pettersson H, Kiessling KH. 1976. Metabolism of aflatoxin by intact rumen fluid, rumen protozoa and rumen bacteria. Swedish Journal of Agricultural Research 6:161.
  23. Pulina G, Battacone G, Brambilla G, Cheli F, Danieli PP, Masoero F, Pietri A, Ronchi B. 2014. An update on the safety of foods of animal origin and feeds. Italian Journal of Animal Science 13:845-856.
  24. Punia BS, Singh S. 2001. Buffalo calf feeding and management. Buffalo Bulletin 20:3-11.
  25. Singh R, Shamsudeen P. 2008. Aflatoxigenic potential of Aspergillus parasiticus MTCC 411 and Aspergillus parasiticus NRRL 2999 under laboratory conditions. Indian Journal of Poultry Science 43:245-246.
  26. Smith MC, Madec S, Coton E, Hymery N. 2016. Natural co-occurrence of mycotoxins in foods and feeds and their in vitro combined toxicological effects. Toxins 8:94. https://doi.org/10.3390/toxins8040094
  27. Van Soest PJ, Robertson JB. 1985. Analysis of forages and fibrous foods. A laboratory manual for animal science. Departmant of Animal Science, Cornell University, NY, USA.
  28. Van Soest PJ, Robertson JB, Lewis BA. 1991. Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74:3583-3597. https://doi.org/10.3168/jds.s0022-0302(91)78551-2
  29. Westlake K, Mackie RI, Dutton MF. 1989. In vitro metabolism of mycotoxins by bacterial protozoal and ovine ruminal fluid preparations. Animal Feed Science and Technology 25:169-178. https://doi.org/10.1016/0377-8401(89)90117-X
  30. Yeanpet C, Thamrongyoswittayakul C, Wachirapakorn C, Songsermsakul P, Somphon N, Wongnen C. 2018. Efficacy of mycotoxin adsorbents on aflatoxin B1 decontamination and in vitro rumen fermentation. Prawarun Agriculture Journal 15:260-268.

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