Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Nitric oxide, 8-hydroxydeoxyguanosine, and total antioxidant capacity in human seminal plasma of infertile men and their relationship with sperm parameters

  • Gholinezhad, Maryam (Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences) ;
  • Aliarab, Azadeh (Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences) ;
  • Abbaszadeh-Goudarzi, Ghasem (Department of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences) ;
  • Yousefnia-Pasha, Yousefreza (Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences) ;
  • Samadaian, Niusha (Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences) ;
  • Rasolpour-Roshan, Korush (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
  • Aghagolzadeh-Haji, Hemat (Cellular and Molecular Biology Research Center, Babol University of Medical Sciences) ;
  • Mohammadoo-Khorasani, Milad (Department of Clinical Biochemistry, Faculty of Medicine, Gonabad University of Medical Sciences)
  • Received : 2019.07.12
  • Accepted : 2019.09.23
  • Published : 2020.03.31
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Abstract

Objective: Oxidative stress plays a key role in the pathogenesis of male infertility. But, the adverse effects of oxidative biomarkers on sperm quality remain unclear. This study aimed to investigate the levels of nitric oxide (NO), 8-hydroxydesoxyguanosine (8-OHdG), and total antioxidant capacity (TAC) oxidative biomarkers in seminal plasma and their relationship with sperm parameters. Methods: A total of 77 volunteers participated in the study, including fertile (n = 40) and infertile men (n = 37). NO, 8-OHdG, and TAC levels were measured using the ferric reducing ability of plasma, Griess reagent method and an enzyme-linked immunosorbent assay kit, respectively. Results: The mean values of sperm parameters in the infertile group were significantly lower than those in the fertile group (p< 0.001). The mean 8-OHdG in the seminal plasma of infertile men was significantly higher (p= 0.013) than those of controls, while the mean TAC was significantly lower (p= 0.046). There was no significant difference in NO level between the two groups. The elevated seminal 8-OHdG levels were negatively correlated with semen volume, total sperm counts and morphology (p< 0.001, p= 0.001 and p= 0.052, respectively). NO levels were negatively correlated with semen volume, total sperm counts and morphology (p= 0.014, p= 0.020 and p= 0.060, respectively). Positive correlations between TAC and both sperm count and morphology (p= 0.043 and p= 0.025, respectively) were also found. Conclusion: These results suggested that increased levels of NO and 8-OHdG in seminal plasma could have a negative effect on sperm function by inducing damage to the sperm DNA hence their fertility potentials. Therefore, these biomarkers can be useful in the diagnosis and treatment of male infertility.

Keywords

References

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