Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Immunogenicity of Exosomes from Dendritic Cells Stimulated with Toxoplasma gondii Lysates in Ocularly Immunized Mice

  • Jung, Bong-Kwang (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Kim, Eun-Do (Department of Ophthalmology, Severance Eye Hospital, Severance Hospital, Institute of Vision Research, Yonsei University College of Medicine) ;
  • Song, Hyemi (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Chai, Jong-Yil (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Seo, Kyoung Yul (Department of Ophthalmology, Severance Eye Hospital, Severance Hospital, Institute of Vision Research, Yonsei University College of Medicine)
  • Received : 2019.12.20
  • Accepted : 2020.03.23
  • Published : 2020.04.30
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Abstract

Immunogenicity of dendritic cell-derived exosomes stimulated with Toxoplasma gondii lysates (TLA exo), mixed with cholera toxin as an adjuvant, was investigated in mice immunized via 2 mucosal routes (ocular vs intranasal). BALB/c mice were injected 3 times with TLA exo vaccine at 2 week interval, and the levels of IgG in serum and IgA in tear, saliva, feces, and vaginal wash were measured. To observe the expression of T. gondii-specific B1 gene, mice infected with ME49 T. gondii cysts were immunized with TLA exo or PBS exo (not stimulated with TLA), and their brain tissues were examined. The mice vaccinated via intranasal route elicited significantly higher humoral and mucosal immune responses compared with mice treated with PBS alone. Also, mice immunized via ocular route (by eyedrop) induced significantly higher T. gondii-specific IgG in serum and IgA in tear and feces in comparison with PBS controls. B1 gene expression was significantly lower in TLA exo vaccinated mice than in PBS or PBS exo vaccinated mice. These results demonstrated that ocular immunization of mice with TLA exo vaccine has the potential to stimulate systemic or local antibody responses. This study also highlighted an advantage of an eyedrop vaccine as an alternative for T. gondii intranasal vaccines.

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