대한한의학방제학회지 (Herbal Formula Science)

  • 제28권1호
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  • Pages.81-90
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  • 2020
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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Icariin의 멜라닌합성 촉진 작용

Icariin promotes melanin synthesis

  • 차수빈 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 박설아 (원광대학교 대학원 뷰티디자인학과) ;
  • 강리아민주 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 한의과대학 해부학교실) ;
  • 문연자 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Cha, Su Bin (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Park, Seol A (Department of Beauty Design Graduate School, Wonkwang University) ;
  • Kang, Lea Minju (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Woo, Won Hong (Deptartment of Anatomy, College of Korean Medicine, Wonkwang University) ;
  • Mun, Yeun Ja (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University)
  • 투고 : 2020.02.12
  • 심사 : 2020.02.26
  • 발행 : 2020.02.28
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초록

Objectives : This study was conducted to investigate the effects of major constituents of Epimedium koreanum Nakai (Icariin, epimedium A, epimedium B, and epimedium C) on melanin synthesis. Methods : We measured melanin contents, tyrosinase activity, and expression of Rab27a in B16F10 cells cultured with Epimedium koreanum Nakai ethanol extract (EKN) and their major constituents. After treatment with H89 and dibutyryl cAMP, which inhibit or promote the activation of PKA, we observed changes in melanin synthesis and tyrosinase activity stimulated by EKN. Results : Among them, EKN and icariin enhanced tyrosinase activity and melanin contents. We confirmed that EKN augmented melanin synthesis via cAMP/PKA pathway. Icariin-induced tyrosinase activity and melanin content were attenuated by PKA inhibitor H89, while melanogenic effect of icariin was further augmented by cAMP analog, dbc AMP. However, icariin did not affect the expression of small GTPase Rab27a involved in melanosome transport. Conclusions : These results suggest that icariin promotes melanogenesis through cAMP/PKA pathway but does not affect small GTPase Rab27a.

키워드

참고문헌

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