DOI QR코드

DOI QR Code

Effect of Banggibongnyeongtang on the immunohistological change in LPS-induced depression rats

방기복령탕(防己茯苓湯)이 백서에서 LPS로 유도된 우울증에서 면역 조직학적 변화에 미치는 효과

  • Park, Sung jun (Department of Formulae Pharmacology, College of Korean Medicine, Gachon University) ;
  • Lee, Tae Hee (Department of Formulae Pharmacology, College of Korean Medicine, Gachon University)
  • 박성준 (가천대학교 한의과대학 방제학교실) ;
  • 이태희 (가천대학교 한의과대학 방제학교실)
  • Received : 2020.02.15
  • Accepted : 2020.02.24
  • Published : 2020.02.28

Abstract

Objective : This study is accomplished in order to investigate the effect of banggibongnyeongtang on the immunohistological change in LPS-induced depression rats to confirm the histological result of the previous behavioral and biochemical effect. Methods : LPS 5 ㎍ was injected to lateral ventricle and experimental groups were administered BBT intraperitoneally. The concentration of 5-HT in the Medial Prefrontal Cortex, Striatum, Hippocampus, Amygdala was measured by ELISA. IL-1β, TNF-α mRNA and BDNF mRNA expression in the hippocampus was examined by RT-PCR. Result : BBT enhanced 5-HT concentration at all part of brain but no significantly difference at medial prefrontal cortex and striatum. LPS+BBT400 group increased 5-HT concentration significantly than LPS group at hippocampus and amygdala (p<0.05). BBT decreased IL-1β mRNA expression dose dependently but only with significantly decrease in LPS+BBT400 group than LPS group's in Hippocampus (p<0.05). But BBT did not decrease TNF-α mRNA expression significantly in Hippocampus. BBT increased the expression of BDNF mRNA at hippocampus and LPS+BBT400 group significantly increased comparing with LPS group does (p<0.05). Conclusion : It is postulated that the anti-depressant effect of BBT can be validated through the anti-inflammatory effect, 5-HT concentration increase, and the neuro-protective effect mediated by BDNF by combining the results of the previous report about the behavioral and biochemical effect.

Keywords

References

  1. Belmaker RH, Agam G. Major Depressive Disorder. The new england journal of medicine. 2008;358(1):55-68 https://doi.org/10.1056/NEJMra073096
  2. Kim RH. 2018 Death Causes Statistics. 2019 sep 24. Availabel from; URL:http://kostat.go.kr/portal/korea/kor_nw/1/6/2/index.board?bmode=read&bSeq=&aSeq=377606&pageNo=1&rowNum=10&navCount=10&currPg=&searchInfo=&sTarget=title&sTxt=
  3. Millan J, Mark. Multi-target strategies for the improved treatment of depressive states: Conceptual foundations and neuronal substrates, drug discovery and therapeutic application. Pharmacology & Therapeutics. 2006;110(2):135-370 https://doi.org/10.1016/j.pharmthera.2005.11.006
  4. Jeon SW, Kim YK. Inflammation-induced depression: Its pathophysiology and therapeutic. Journal of Neuroimmunology. 2017;313:92-98 https://doi.org/10.1016/j.jneuroim.2017.10.016
  5. SJ Park, TH Lee. Effect of banggibongnyeongtang on LPS-induced Depression in rats. Herbal Formula Science. 2019;27(2):137-149. https://doi.org/10.14374/HFS.2019.27.2.137
  6. Lee BB, Sur BJ, Cho SG, Yeom MJ, Shim IS, Lee HJ, et al. Wogonin Attenuates Hippocampal Neuronal Loss and Cognitive Dysfunction in Trimethyltin-Intoxicated Rats. Biomolecules & Therapeutics.2016;24(3):328-337 https://doi.org/10.4062/biomolther.2015.152
  7. Lee JE, Kwon YJ, Cho SH. A Review of Clinical Studies with Herbal Medicine for Depression - Based on Randomized Controlled Clinical Trial. The Journal of Oriental Neuropsychiatry. 2011;22(4):31-39 https://doi.org/10.7231/JON.2011.22.4.031
  8. Hindmarch I. Beyond the monoamine hypothesis: mechanisms, molecules and methods. European Psychiatry. 2002;17(3):294-299 https://doi.org/10.1016/S0924-9338(02)00674-0
  9. Pigott HE, Leventhal AM, Alter GS, Boren JJ. Efficacy and Effectiveness of Antidepressants: Current Status of Research. Psychother Psychosom. 2010;79(5):267-279 https://doi.org/10.1159/000318293
  10. Raison CL, Capuron L, Miller AH. Cytokines sing the blues: inflammation and the pathogenesis of depression. TRENDS in Immunology. 2006;27(1):24-31 https://doi.org/10.1016/j.it.2005.11.006
  11. Miller AH, Maletic V, Raison CL. Inflammation and Its Discontents: The Role of Cytokines in the Pathophysiology of Major Depression. Biological Psychiatry. 2009;65(9):732-741 https://doi.org/10.1016/j.biopsych.2008.11.029
  12. Dooley LN, Kuhlman KR, Robles TF, Eisenberger NI, Craske MG, et al. The role of inflammation in core features of depression: Insights from paradigms using exogenously-induced inflammation. Neuroscience and Biobehavioral Reviews. 2018;94:219-237 https://doi.org/10.1016/j.neubiorev.2018.09.006
  13. Hanbangyangnihang gyojaepyeonchanwiwonhoe. Hanbangyangnihak. 4thedition.Seoul:Sinilbukseu. 2015:193-8.
  14. HongJW, YangGE, KimYB, EomSH, LewJH, Kangh. Anti-inflammatory activity of cinnamon water extract in vivo and in vitro LPSinduced models. ISCMR. 2012;12:237doi.org/10.1186/1472-6882-12-237
  15. Ressler KJ, Nemeroff CB. Role of serotonergic and noradrenergic systems in the pathophysiology of depression and anxiety disorders. Depress Anxiety. 2000;12Suppl1:2-19. https://doi.org/10.1002/1520-6394(2000)12:1+<2::AID-DA2>3.0.CO;2-4
  16. Sapolsky R, Rivier C, Yamamoto G, Plotsky P, Vale W. Interleukin-1 stimulates the secretion of hypothalamic corticotropin- releasing factor. Science. 1987;238(4826): 522-4 https://doi.org/10.1126/science.2821621
  17. Leonard BE. The HPA and immune axes in stress: the involvement of the serotonergic system. Eur Psychiatry, 2005 20(3),S302-S306. https://doi.org/10.1016/S0924-9338(05)80180-4
  18. Sapolsky R, Rivier C, Yamamoto G, Plotsky P, Vale W. Interleukin-1 stimulates the secretion of hypothalamic corticotropin- releasing factor. Science. 1987;238(4826): 522-4 https://doi.org/10.1126/science.2821621
  19. Berkenbosch F, van Oers J, del Rey A, Tilders F, Besedovsky H. Corticotropinreleasing factor-producing neurons in the rat activated by interleukin-1. Science. 1987;238(4826):524-6 https://doi.org/10.1126/science.2443979
  20. Kruse N, Cetin S, Chan A, Gold R, Luhder F. Differential expression of BDNF mRNA splice variants in mouse brain and immune cells. Journal of Neuroimmunology. 2007Jan;182(1-2):13-21 https://doi.org/10.1016/j.jneuroim.2006.09.001
  21. Wang Y, Zhang TY, Xin J, Li T, Yu H, Li N et al. Differential involvement of brainderived neurotrophic factor in reconsolidation and consolidation of conditioned taste aversion memory. PLoS One. 2012;7(11):e49942. doi: 10.1371 https://doi.org/10.1371/journal.pone.0049942
  22. Chen B, Dowlatshahi D, MacQueen GM, Wang JF, Young LT. Increased hippocampal BDNF immunoreactivity in subjects treated with antidepressant medication. Biol Psychiatry. 2001Aug15;50(4):260-5. https://doi.org/10.1016/S0006-3223(01)01083-6
  23. Sen S, Duman R, Sanacora G. Serum brainderived neurotrophic factor, depression, and antidepressant medications: meta-analyses and implications. Biol Psychiatry. 2008Sep15;64(6):527-32. doi: 10.1016 https://doi.org/10.1016/j.biopsych.2008.05.005
  24. Martinowich K, Lu B. Interaction between BDNF and Serotonin: Role in mood disorders. Neuropsychopharmacology. 2008Jan;33(1):73-83 https://doi.org/10.1038/sj.npp.1301571
  25. Hritcu L, Gorgan LD. Intranigral lipopolysaccharide induced anxiety and depression by altered BDNF mRNA expression in rat hippocampus. Prog Neuropsychopharmacol Biol Psychiatry. 2014Jun3;51:126-32. doi: 10.1016 https://doi.org/10.1016/j.pnpbp.2014.01.016