Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant Activity and Standardization of Extraction Solvents of SJ004

SJ004의 추출용매별 항산화 활성 및 표준화 연구

  • Received : 2020.03.17
  • Accepted : 2020.04.03
  • Published : 2020.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives SJ004 is a natural herbal medicine that contains Acyranthes japonica Nakai and Eucommia ulmoides Oliver traditionally used for joint and spinal diseases. This study aimed to establish an efficient method of extracting SJ004 to standardize using the yield, high-performance liquid chromatography (HPLC), and antioxidant assay. Methods SJ004 was extracted with distilled water, 70% and 100% of ethyl alcohol (EtOH). The method validation of 20-hydroxyecdysone and pinoresinol diglucoside was determined by HPLC-photo diode array and the content of SJ004 was calculated. The antioxidant activity of each extract was compared and measured using total flavonoids, total phenolic compounds, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power according to the standard protocol. Results The yield was highest in pure water extract and lowest in 100% EtOH. But, the content of marker compounds indicating 20-hydroxyecdysone and pinoresinol diglucoside was highest in 100% EtOH extract. In the physiological activity measurement using antioxidant activity, 100% ethanol extract was highest. The limit of detection indicating 20-hydroxyecdysone and pinoresinol diglucoside were analyzed 0.33 ㎍/mL, 0.1616 ㎍/mL, and the limit of quantification were analyzed 1.01 ㎍/mL and 0.49 ㎍/mL respectively. Conclusions The experimental results showed that the extraction conditions have a significant effect on content of marker compounds and antioxidant activity. As a result of method validation, SJ004 was standardized by 20-hydroxyecdysone and pinoresinol diglucoside.

Keywords

References

  1. Uhm WS, Yun JE, Park YW, Kim HR, Nam JJ, Lee HS, Kim TH, Jun JB, Yoo DH, Bae SC. The prevalence of self-reported arthritis and its epidemiologic characteristics in Korea. Journal of the Korean Rheumatism Association. 2004;11(2):116-25.
  2. Kutuk O, Basaga H. Inflammation meets oxidation: NF-kappaB as a mediator of initial lesion development in atherosclerosis. Trends in Molecular Medicine. 2003;9(12):549-57. https://doi.org/10.1016/j.molmed.2003.10.007
  3. Sanchez-Borges M. NSAID hypersensitivity(respiratory, cutaneous, and generalized anaphylactic symptoms). Medical Clinics of North America. 2010;94(4):853-64. https://doi.org/10.1016/j.mcna.2010.03.005
  4. Ju MS, Jeong HU, Kim HG, Park GH, Youn YS, Kim YO, Kim SY, Oh MS. Anti-nociceptive and anti-inflammatory effects of geranii herba. Korean Journal of Herbology. 2010;25(3):97-101.
  5. Kim SN, Lee EJ, Lee HJ, Nam GS, Kim HS, Hwang SW, Hwang SY. Effect on inflammatory-cytokines production inhibition and analgesic activity of perilla frutescens extracts. Jouranal of Physiology & Pathology in Korean Medicine. 2006;20(2):414-9.
  6. Park MJ, Lee KY, Park KH, Chung AK, Shin DH. The clinical study on the effect of sulguanjul-bang no.1 on osteoarthritis of knee joint. Korean Journal of Acupuncture Research. 2004;21(3):249-64.
  7. Vetrichelvan T, Jegadeesan M. Effect of alcohol extract of achyranthes aspera linn. on acute and subacute inflammation. Phytotherapy Research. 2003;17(1):77-9. https://doi.org/10.1002/ptr.1070
  8. Chakraborty A, Brantner A, Mukainaka T, Nobukuni Y, Kuchide M, Konoshima T, Tokuda H, Nishino H. Cancer chemopreventive activity of achyranthes aspera leaves on epstein-barr virus activation and two-stage mouse skin carcinogenesis. Cancer Letters. 2002;177(1):1-5. https://doi.org/10.1016/S0304-3835(01)00766-2
  9. Han DS. Pharmacognosy. 2th ed. Seoul:Dongmyeong Publishers. 2014:182-4.
  10. Jung SM, Choi SI, Park SM, Heo TR. Antimicrobial effect of achyranthes japonica Nakai extracts against clostridium difficile. Korean Journal of Food Science and Technology. 2007;39(5):564-8.
  11. Choi DH, Lee SJ, Jeong HC, Kim KM, Kwon MY, Lee SH, Sohn JH. Anti-osteoarthritis and anti-arthrodynia effects of a mixture of fermented achyranthes japonica Nakai, Angelica gigas Nakai, and Eucommia ulmoides Oliver extracts on monosodium iodoacetate-induced arthritis in rats. Korean Journal of Food Science and Technology. 2019;48(11):1179-85.
  12. Seo BI, Kwon DY, Choi HY, Lee JH, Sook OM, Bu YM. Medicinal herbology. 1st ed. Seoul:Younglim Publishers. 2012:572-4.
  13. Luo LF, Wu WH, Zhou YZ, Yan J, Yang GP, Ouyang DS. Antihypertensive effect of Eucommia ulmoides Oliv. extracts in spontaneously hypertensive rats. Journal of Ethnopharmacology. 2010;129(2):238-43. https://doi.org/10.1016/j.jep.2010.03.019
  14. Youn Y, Baek HI, Jin HY, Jeong DY, Shen L, Joo JC, Park SJ. Randomized double-blind human trial to evaluate efficacy and safety of Rhus verniciflua stokes (Lacca Sinica Exsiccata) and Eucommia ulmoides Oliver (Eucommiae Cortex) extract combination (ILF-RE) on improvement of liver function. Korean Journal of Herbology. 2020;35(1):45-55.
  15. Kim SH, Kim IC. Antioxidative properties and whitening effects of the Eucommiae cortex, Salviae miltiorrhizae radix, Aurantii nobilis pericarpium and Cnidii rhizome. Journal of the East Asian Society of Dietary Life. 2008;18(4):618-23.
  16. Kim HK, Kim YE, Do JR, Lee YC, Lee BY. Antioxidative activity and physiological activity of some Korean medicinal plants. Korean Journal of Food Science and Technology. 1995;27(1):80-5.
  17. Jeon WJ, Shin GJ, Lee WC. An experimental studies on the alleviation effects of Daebangpoongtang(大防風湯) in LPS-induced arthritis. Journal of Oriental Medicine Drug R &D Center. 2000;9(9):35-49.
  18. Je YM, Yoo DY. Effects of Dokhwalgisaengtang-gami water extract on osteoclast differentiation and osteoblast function in RANKL-induced RAW 264.7 cell. Journal of Korean Obstetrics & Gynecology. 2013;26(2):1-16. https://doi.org/10.15204/jkobgy.2013.26.2.001
  19. Park H, Kim YH. Study on the anti-inflammatory activity and mechanism of medicinal plants used in the treatment of arthritis. Korean Journal of Clinical Laboratory Science. 2016;48(3):176-82. https://doi.org/10.15324/kjcls.2016.48.3.176
  20. Kwon SS, Choi SK, Kuen JD, Lee JW, Cheol SS, Choi HL, Oh YS, Seong ES. Functional assessment of complex composition based on Achyranthes japonica Nakai. Korean Journal of Food Engineering Progess. 2019;23(2):104-11.
  21. Park JS, Park GY, Choi HG, Kim SJ, Kim JH, Park MC, Kim YK, Han SY, Jo EH. Inhibitory effects of water extracts of eucommiae cortex and psoraleae semen alone and in combination on osteoclast differentiation and bone. Journal of Acupuncture Research. 2017;34(2):1-18. https://doi.org/10.13045/acupunct.2017079
  22. Jang GY, Kim HY, Lee SH, Kang YR, Hwang IG, Woo KS, Kang TS, Lee JS, Jeong HS. Effects of heat treatment and extraction method on antioxidant activity of several medicinal plants. Journal of the Korean Society of Food Science and Nutrition. 2012;41(7):914-20. https://doi.org/10.3746/JKFN.2012.41.7.914
  23. Koh WI, Lee JH, Ha IH, Chung HJ, Lee IH, Lee JW, Kim EJ, Gang BG, Jeon SH, Cho YK, Kim MJ. Salt-water processing-dependent change in anti-oxidative and anti-inflammatory effects of cortex eucommiae. J Korean Med Rehabil. 2017;27(2):29-38. https://doi.org/10.18325/jkmr.2017.27.2.29
  24. Kim YH, Sung HS, Kim WJ. Effect of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean Journal of Food Science and Technology. 1993;25(3):204-9.
  25. Arnao MB, Cano A, Acosta M. The hydrophilic and lipophilic contribution to total antioxidant activity. Journal of Food Chemistry. 2001;73(2):239-44. https://doi.org/10.1016/S0308-8146(00)00324-1
  26. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical Biochemistry. 1996;239(1):70-6. https://doi.org/10.1006/abio.1996.0292
  27. Ahn JY, Kee JY, Kim JH, Park HJ, Kim HJ, Lee JH, Kim YK. A review of anti-osteoporosis effects of herbal extracts in Korean journals. Korean Journal of Oriental Medicinal Prescription. 2008;16(2):47-71.
  28. Yu HE, Dela Paz LMM, Bae YJ, Lee DH, Park JS, Kwak HS, Kim HK, Lee JS. Screening and extraction condition of anti-aging bioactive substances from medicinal plants. Journal of Korean Society of Food Science and Nutrition. 2005;34(8):1136-42. https://doi.org/10.3746/JKFN.2005.34.8.1136
  29. Lee IH, Chung HJ, Shin JS, Ha IH, Kim MR, Koh W, Lee JH. Influence of boiling duration of GCSB-5 on index compound content and antioxidative and anti-inflammatory activity. Pharmacognosy Magazine. 2017;13(51):418-24. https://doi.org/10.4103/pm.pm_425_16
  30. Na GM, Han HS, Ye SH, Kim HK. Physiological activity of medicinal plant extracts. Korean Journal of Food Preservation. 2004;11(3):388-93.
  31. Lee JW, Lee SK, Do JH, Yang JW. Detremination of total phenolic compounds from Korean red ginseng and their extraction condition. Journal of Ginseng Research. 2000;24(2):64-7.
  32. Kehrer JP, Klotz LO. Free radicals as mediators of tissue injury and disease. Critical Reviews in Toxicology. 1993;23(1):21-48. https://doi.org/10.3109/10408449309104073
  33. Lee JS, Lee MK, Kim YK, Kim KE, Hyun KY. Attenuant effects of Hovenia dulcis extract on inflammatory orifacial pain in rats. The Korean Jounal of Academia- Industrial Cooperation Society. 2014;15(8):5088-94. https://doi.org/10.5762/KAIS.2014.15.8.5088
  34. Choi KB, Nam KW, Kim GY, Sim KC, Kim EJ. Effect of swimming exercise and achyranthe radix extracts on inflammatory and pain responses in rheumatoid arthritis rats. Jounal of Physiology & Pathology in Korean Medicine. 2009;23(1):113-20.

Cited by

  1. Network Pharmacological Dissection of the Mechanisms of Eucommiae Cortex-Achyranthis Radix Combination for Intervertebral Disc Herniation Treatment vol.16, pp.10, 2020, https://doi.org/10.1177/1934578x211055024