Journal of Practical Agriculture & Fisheries Research (현장농수산연구지)

Korea National University of Agriculture and Fisheries (국립한국농수산대학교 교육개발센터)
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Anti-inflammatory and Cytotoxic Screening Evaluation of Macroalgae Resources

국내 해조류 자원의 항염증 및 세포독성 스크리닝 평가

  • Kim, C.W. (Department of Aquaculture, Korea National College of Agriculture and Fisheries) ;
  • Chang, K.J. (Department of Medicinal and Industrial Crops, Korea National College of Agriculture and Fisheries) ;
  • Kim, Y.B. (Department of Medicinal and Industrial Crops, Korea National College of Agriculture and Fisheries) ;
  • Kim, D.H. (Department of Medicinal and Industrial Crops, Korea National College of Agriculture and Fisheries) ;
  • Chae, C.J. (Department of General Education, Korea National College of Agriculture and Fisheries) ;
  • Choi, H.G. (Division of Biological Science, Wonkwang University) ;
  • Koo, H.J. (Department of Medicinal and Industrial Crops, Korea National College of Agriculture and Fisheries)
  • 김철원 (국립한국농수산대학 수산생물양식학과) ;
  • 장광진 (국립한국농수산대학 특용작물학과) ;
  • 김연복 (국립한국농수산대학 특용작물학과) ;
  • 김동현 (국립한국농수산대학 특용작물학과) ;
  • 채철주 (국립한국농수산대학 교양공통과) ;
  • 최한길 (원광대학교 자연과학대학생명과학부) ;
  • 구현정 (국립한국농수산대학 특용작물학과)
  • Received : 2020.10.21
  • Accepted : 2020.11.29
  • Published : 2020.12.28
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Abstract

In this study, the anti-inflammatory and cytotoxic effects of hot-water extracts from 10 kinds of macroalgae in Korea were investigated. It was selected materials in consideration of biological activity and industrial potential as follows: Caulerpa okamurae; Codium fragile; Ulva australis; Ishige foliacea; Saccharina japonica; Sargassum horneri; Undaria pinnatifida; Gloiopeltis tenax; Gracilaria verrucosa; Porphyra tenera. Results showed that S. japonica and G. tenax significantly decreased NO productionn in LPS-stimulated Raw 264.7 cells at concentrations of 100, 1000 ㎍/mL and 1000 ㎍/mL, respectively. However, most of the other macroalgae used in the experiment did not affect NO production. It was observed that all macroalgae extracts except for the highest concentration (1000 ㎍/mL) treatment group of P. tenera did not affect the viability in Raw 264.7 cells. In addition, there was not significant decrease in cell viability by macroalgae extracts treatment in HINAE cells. These results suggest that S. japonica and G. tenax could be used as potential safe natural anti-inflammatory agents for food and feed additives. Also, the results of this study are expected to be used as basic data for the development of functional materials for 10 kinds of macroalgae resources in Korea.

본 연구에서는 해조류 자원의 산업적 활용 소재로서의 가치 및 기능성 식품 및 사료 첨가 소재로의 개발을 위한 기초 자료를 제시하고자 국내에 서식하는 10종의 해조류 열수추출물을 이용하여 항염증 활성 및 마우스 대식세포주와 어류 세포주의 세포생존율에 미치는 영향을 확인하였다. 그 결과 S. japonica 100, 1000 ㎍/mL과 G. tenax 1000 ㎍/mL 농도에서 마우스 대식세포주를 LPS로 자극하였을 때 생성되는 NO를 유의적으로 감소시키는 것을 관찰하였으며, 나머지 8종의 해조류 추출물은 실험 농도에서 LPS에 의한 NO 생성에 영향을 미치지 않았다. 고농도에서 항염증 활성을 보인 S. japonica 및 G. tenax 열수추출물은 염증 인자를 조절할 수 있는 기능성 후보 소재로서 분획별 생리활성과 유효성분 규명의 연구가 요구된다. 본 연구 대상 해조류 추출물에 대한 안전성 기초 자료로서 마우스 대식세포 및 넙치 배아 유래 세포 세포생존율에 미치는 영향을 평가한 결과, P. tenera 고농도 (1000 ㎍/mL) 처리군을 제외하고 모든 해조류 추출물은 마우스 대식세포의 생존율에 영향을 주지 않았으며, 모든 해조류 추출물에서 5000 ㎍/mL까지 넙치 배아유래 세포에 대한 세포독성이 나타나지 않았다. 본 연구 결과는 후속 연구를 통해 해조류의 다양하고 복잡한 구조의 생물 활성 화합물의 생체 내 메커니즘을 규명함으로써 기능성 소재 개발을 위한 기초자로료 활용될 수 있을 것으로 기대하며, 본 연구의 대상 10종의 국내 해조류 자원 중 S. japonica과 G. tenax은 잠재적인 천연 항염증제로서 안전한 식품 및 사료 첨가 소재로서의 가능성을 제시한다.

Keywords

References

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