Journal of Practical Agriculture & Fisheries Research (현장농수산연구지)

Korea National University of Agriculture and Fisheries (국립한국농수산대학교 교육개발센터)
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Analysis of Pregnancy-Associated Plasma Protein-A (PAPP-A) in Porcine Neonatal Testis

미성숙 돼지 정소 내에서의 pregnancy-associated plasma protein-A 특성 분석

  • Lee, W.Y. (Department of Beef Science, Korea National College of Agriculture and Fisheries) ;
  • Cho, K.H. (Department of Dairy Science, Korea National College of Agriculture and Fisheries) ;
  • Yeo, J.M. (Department of Dairy Science, Korea National College of Agriculture and Fisheries) ;
  • Shin, Y.K. (Department of Agriculture and Fisheries Business, Korea National College of Agriculture and Fisheries) ;
  • Park, J.K. (Department of Swine Science, Korea National College of Agriculture and Fisheries)
  • 이원영 (국립한국농수산대학 한우학과) ;
  • 조광현 (국립한국농수산대학 낙농학과) ;
  • 여준모 (국립한국농수산대학 낙농학과) ;
  • 신용광 (국립한국농수산대학 농수산비즈니스학과) ;
  • 박진기 (국립한국농수산대학 양돈학과)
  • Received : 2020.03.19
  • Accepted : 2020.06.19
  • Published : 2020.06.30
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Abstract

The identify of biomarkers in living tissues is useful to understand the characteristics and functions of the cells. Proteins such as protein gene product 9.5, promyelocytic leukemia zinc finger, NANOG, and stage-specific embryonic antigen-1 have been identified as markers for porcine undifferentiated spermatogonia. In this study, the expression of insulin-like growth factor binding proteins (IGFBPs), a newly discovered porcine spermatogonia marker and pregnancy-associated plasma protein-A (PAPP-A), a protein regulator of IGFBPs, were characterized in 5-day-old porcine testis. To analyze the function of IGFBPs, RT-PCR was performed. IGFBP 2, 3, 4, and 6 were detected in porcine spermatogonia and PAPP-A was detected in basement regions in 5day old porcine seminiferous tubules. PAPP-A was not expressed in spermatogonia, but it was expressed in Sertoli cells. These results suggest that the expression of PAPP-A protein in Sertoli cells may regulate the development and differentiation of testicular cells through the IGF axis in porcine neonatal testis.

생체 조직 내에서 표지인자의 발견은 해당 세포의 특성과 기능을 이해하는 데 매우 중요하다. 기존에 밝혀진 돼지 정원세포의 표지인자로는 PGP9.5, PLZF, NANOG, SSEA1 등의 단백질이 알려져 있다. 본 연구에서는 최근 새로이 발굴된 돼지 정원세포 표지인자인 IGFBPs 의 기능을 분석하기 위해 IGFBPs 의 발현과 이를 조절하는 단백질인 PAPP-A 단백질의 발현을 5일령 돼지 정소에서 확인하였다. IGFBP 2, 3, 4, 6 의 발현이 돼지 정원세포 특이적으로 높게 나타났으며 PAPP-A의 발현은 세르톨리세포 특이적으로 나타났다. PAPP-A 의 발현을 PGP9.5, GATA4 등의 표지 인자와 함께 확인해 본 결과 PGP9.5를 발현하는 정원세포에서는 발현하지 않았으며, 세정관 내의 세르톨리세포 특이적으로 발현하였다. 이러한 사실로 미루어 볼 때 세르톨리세포에서 발현하는 PAPP-A 단백질은 정원세포에서 발현하는 IGFBPs 의 조절을 통하여 알려진 바와 같이 IGF axis 를 통해 정소내 세포들의 발달 및 분화를 조절할 것으로 판단된다.

Keywords

References

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