Journal of Microbiology and Biotechnology

  • 제30권4호
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  • Pages.615-621
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  • 2020
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Multiple Tolerances and Dye Decolorization Ability of a Novel Laccase Identified from Staphylococcus Haemolyticus

  • Li, Xingxing (College of Animal Science and Technology, Northwest A&F University) ;
  • Liu, Dongliang (College of Animal Science and Technology, Northwest A&F University) ;
  • Wu, Zhaowei (College of Animal Science and Technology, Northwest A&F University) ;
  • Li, Dan (College of Animal Science and Technology, Northwest A&F University) ;
  • Cai, Yifei (College of Animal Science and Technology, Northwest A&F University) ;
  • Lu, Yao (College of Animal Science and Technology, Northwest A&F University) ;
  • Zhao, Xin (College of Animal Science and Technology, Northwest A&F University) ;
  • Xue, Huping (College of Animal Science and Technology, Northwest A&F University)
  • 투고 : 2019.10.28
  • 심사 : 2020.01.20
  • 발행 : 2020.04.28
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초록

Laccases are multicopper oxidases with important industrial value. In the study, a novel laccase gene (mco) in a Staphylococcus haemolyticus isolate is identified and heterologously expressed in Escherichia coli. Mco shares less than 40% of amino acid sequence identities with the other characterized laccases, exhibiting the maximal activity at pH 4.0 and 60℃ with 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) as a substrate. Additionally, the Mco is tolerant to a wide range of pH, heavy metal ions and many organic solvents, and it has a high decolorization capability toward textile dyes in the absence of redox mediators. The characteristics of the Mco make this laccase potentially useful for industrial applications such as textile finishing. Based on BLASTN results, mco is found to be widely distributed in both the bacterial genome and bacterial plasmids. Its potential role in oxidative defense ability of staphylococci may contribute to the bacterial colonization and survival.

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참고문헌

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