Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effect of stigmasterol in liposome bilayer on the stabilization of encapsulated ascorbic acid

리포솜 이중층의 stigmasterol이 포집된 ascorbic acid의 안정성에 미치는 영향

  • 이동욱 (경남대학교 바이오융합학부) ;
  • 박혜원 (경남대학교 바이오융합학부) ;
  • 이승철 (경남대학교 바이오융합학부)
  • Received : 2020.02.27
  • Accepted : 2020.04.02
  • Published : 2020.04.30
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Abstract

The effect of stigmasterol (SS), a phytosterol, in the liposome bilayer on the stability of encapsulated ascorbic acid (ASA) was evaluated. Liposomes, consisting of phosphatidylcholine (PC) and SS, and ASA were encapsulated by the dehydration/rehydration method. The average particle size of the liposome increased with increasing SS content. SS significantly increased the stability of encapsulated ASA. For example, ASA remaining in the liposomes of 100:0, 90:10, and 70:30 (PC:SS, w/w) ratios was 34.12%, 49.88%, and 58.58%, respectively, after storage for 8 days at 4℃, while only 7.66% ASA remained in the buffer under the same conditions. These results indicated that SS in liposomes increased the stability of encapsulated ASA.

SS은 식물성 스테롤로서 인체에 유익한 생리활성을 나타낸다. 리포솜 인지질을 PC과 SS로 구성하고, ASA를 탈수/재수화 방법에 의해 포집시킨 후, ASA의 안정성을 분석하였다. 리포솜의 평균 크기는 SS 함량이 증가할수록 증가하였으며, SS는 포집된 ASA의 안정성을 향상시켰다. 예를 들어, 완충용액에 존재하는 ASA는 4℃에서 8일간 저장하면 7.66%가 잔존하지만, 100:0, 90:10, 70:30 (PC/SS)로 조성된 리포솜에 포집된 ASA는 같은 조건에서 각각 34.12, 49.88, 58.58%가 잔존하였다. 이러한 결과는 리포솜에서 SS가 포집된 ASA의 안정성을 향상시킴을 의미한다.

Keywords

References

  1. Akbarzadeh A, Rezaei-Sadabady R, Davaran S, Joo SW, Zarghami N, Hanifehpour Y, Samiei M, Kouhi M, Nejati-Koshki K. Liposome: classification, preparation, and applications. Nanoscale Res. Lett. 8: 102 (2013) https://doi.org/10.1186/1556-276X-8-102
  2. Arrigoni O, De Tulino MC. Ascorbic acid: much more than just an antioxidant, Biochim. Biophys. Acta 1569: 1-9 (2002) https://doi.org/10.1016/S0304-4165(01)00235-5
  3. Beutler HO. L-ascorbate and L-dehydroascorbate. Vol. 1, pp. 376-385. In: Methods in Enzymatic Analysis. 3rd ed., Bermeyer HV (ed). Verlag Chemie., Basel, Switzerland (1984)
  4. Cabral CE, Klein MR. Phytosterols in the treatment of hypercholesterolemia and prevention of cardiovascular diseases. Arq. Bras. Cardiol. 109: 475-482 (2017)
  5. Coderch L, Fonollosa J, De Pera M, Estelrich J, De La Maza A, Parra JL. Influence of cholesterol on liposome fluidity by EPR. Relationship with percutaneous absorption. J. Control Release 68: 85-95 (2000) https://doi.org/10.1016/S0168-3659(00)00240-6
  6. Daraee H, Etemadi A, Kouhi M, Alimirzalu S, Akbarzadeh A. Application of liposomes in medicine and drug delivery. Artif. Cell Nanomed. B. 44: 381-391 (2016) https://doi.org/10.3109/21691401.2014.953633
  7. de Meyer F, Smit B. Effect of cholesterol on the structure of a phospholipid bilayer. Proc. Natl. Acad. Sci. USA. 106: 3654-3658 (2009) https://doi.org/10.1073/pnas.0809959106
  8. Emami S, Azadmard-Damirchi S, Peighambardoust SH, Valizadeh H, Hesari J. Liposomes as carrier vehicles for functional compounds in food sector. J. Exp. Nanosci. 11: 737-759 (2016) https://doi.org/10.1080/17458080.2016.1148273
  9. Herbig AL, Renard CMGC. Factors that impact the stability of vitamin C at intermediate temperature in a food matrix. Food Chem. 220: 444-451 (2017) https://doi.org/10.1016/j.foodchem.2016.10.012
  10. Johnson SM. The effect of charge and cholesterol on the size and thickness of sonicated phospholipid vesicles. Biochim. Biophys. Acta 307: 27-41 (1973) https://doi.org/10.1016/0005-2736(73)90022-9
  11. Kirby CJ, Gregoriadis G. Dehydration-rehydration vesicles, a simple method for high yield drug entrapment in liposome. Biotechnology 2: 979-984 (1984)
  12. Koynova R, Caffrey M. Phases and phase transitions of the phosphatidylcholines. Biochim. Biophys. Acta 1376: 91-145 (1998) https://doi.org/10.1016/S0304-4157(98)00006-9
  13. Kratz M. Dietary cholesterol, atherosclerosis and coronary heart disease. Handb. Exp. Pharmacol. 170: 195-213 (2005) https://doi.org/10.1007/3-540-27661-0_6
  14. Lee SC, Lee KE, Kim JJ, Lim SH. 2005. The effect of cholesterol in the liposome bilayer on the stabilization of incorporated retinol. J. Liposome Res. 15: 157-166 (2005) https://doi.org/10.1080/08982100500364131
  15. Lee SC, Yuk HG, Lee DH, Lee KE, Hwang YI, Ledescher RD. Stabilization of retinol through incorporation into liposome. J. Biochem. Mol. Biol. 35: 358-363 (2002)
  16. Lee YW, Hwang YI, Lee SC. Effect of liposome on the stabilization of ascorbic acid. Korean J. Food Sci. Technol. 31: 280-284 (1999)
  17. Rhim CH, Lee YW, Lee SC, Lee SC. Effect of cholesterol in liposome on the stabilization of encapsulated ascorbic acid. J. Korean Soc. Agric. Chem. Biotechnol. 42: 205-209 (1999)
  18. Tran VV, Moon JY, Lee YC. Liposomes for delivery of antioxidants in cosmeceuticals: Challenges and development strategies. J. Control Release 300: 114-140 (2019) https://doi.org/10.1016/j.jconrel.2019.03.003