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생물반응기 산삼 배양액의 진세노사이드 분석 및 엽채류 응용에 따른 생물학적 변화

Ginsenoside Analysis of Panax ginseng C. A. Meyer Culture Broth in a Bioreactor and Its Application in Inducing Biological Changes in Leafy Vegetables

  • 김창흠 (강원대학교 생물자원과학과) ;
  • 성은수 (수원여자대학교 약용식물과) ;
  • 유지혜 (강원대학교 한방바이오연구소) ;
  • 이재근 (화진화장품 생명공학연구소) ;
  • 유창연 (강원대학교 생물자원과학과)
  • Kim, Chang Heum (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Seong, Eun Soo (Department of Medicinal Plant, Suwon Women's University) ;
  • Yoo, Ji Hye (Bioherb Research Institute, Kangwon National University) ;
  • Lee, Jae Geun (Research Institute of Biotechnology, Hwajin Cosmetics) ;
  • Yu, Chang Yeon (Department of Bio-Resource Sciences, Kangwon National University)
  • 투고 : 2020.02.06
  • 심사 : 2020.04.21
  • 발행 : 2020.04.30

초록

Background: The aim of this study was done to identify whether mass produced wild ginseng culture broth prepared from cultivated wild ginseng roots could have an application in enhancing the agricultural utility value of leafy vegetables. Methods and Results: Leafy vegetables Lactuca sativa and Brassica juncea were treated with wild ginseng culture broth. Plants were examined and treatment (100 ㎖) applied twice a week over an eight week period. Total phenolic and flavonoid content of treated plants was then measured. Wild ginseng culture broth treatment resulted in phenolic and flavonoid content of 0.40 mg·GAE/㎖ and 0.36 mg·QE/㎖, respectively in L. sativa. When treated with wild ginseng culture broth, free radical scavenging ability was found to be higher in both L. sativa and B. juncea whereas antimicrobial activity was found to be higher in B. juncea (625 ㎍/㎖) than in L. sativa. Inorganic element analysis of L. sativa and B. juncea showed that Ca and Mg were higher in the wild ginseng broth treatment group, whereas harmful elements such as As were reduced. Conclusions: Rather than discarding the wild ginseng culture broth, it can be used as a fresh biomaterial by reprocessing it as agricultural products that can promote growth and improve functionality in plants.

키워드

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