Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Protective Effects of Combination of Carthamus tinctorius L. Seed and Taraxacum coreanum on Scopolamine-induced Memory Impairment in Mice

홍화씨와 흰민들레 복합물의 Scopolamine 유도 기억력 손상에 대한 보호 효과

  • Kim, Ji Hyun (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University) ;
  • He, Mei Tong (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University) ;
  • Kim, Min Jo (Institute of Natural Medicine, Toyama Medical and Pharmaceutical University) ;
  • Park, Chan Hum (Farmdew Inc.) ;
  • Lee, Jae Yang (Insan Life Science Research Institute, Insan Inc.) ;
  • Shin, Yu Su (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University)
  • 김지현 (부산대학교 식품영양학과 및 김치연구소) ;
  • ;
  • 김민조 (토야마의과약과대학) ;
  • 박찬흠 ((주)참선진녹즙) ;
  • 이재양 ((주)인산가 기업부설 인산생명과학연구소) ;
  • 신유수 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 조은주 (부산대학교 식품영양학과 및 김치연구소)
  • Received : 2020.03.18
  • Accepted : 2020.04.20
  • Published : 2020.04.30
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Abstract

Background: Alzheimer's disease (AD) is caused by various factors, such as cholinergic dysfunction, regulation of neurotrophic factor expression, and accumulation of amyloid-beta. We investigated whether or not a combination of Carthamus tinctorius L. seed and Taraxacum coreanum (CT) has a protective effect on scopolamine-induced memory impairment in a mouse model. Methods and Results: Mice were orally pretreated with CT (50, 100 and 200 mg/kg/day) for 14 days, and scopolamine (1 mg/kg/day) was injected intraperitoneally before subjecting them to behavior tests. CT-administered mice showed better novel object recognition and working memory ability than scopolamine-treated control mice. In T-maze and Morris water maze tests, CT (100 and 200 mg/kg/day) significantly increased space perceptive ability and occupancy to the target quadrant, respectively. In addition, 100 and 200 mg/kg/day of CT attenuated cholinergic dysfunction through inhibition of butyryl cholinesterase in brain tissue. Furthermore, CT-administered mice showed higher cyclic adenosine monophosphate-response element-binding protein (CREB) levels and lower amyloid precursor protein (APP) levels compared to scopolamine-treated control mice. Conclusions: CT improved scopolamine-induced memory impairment through inhibition of cholinergic dysfunction, up-regulation of CREB, and down-regulation of APP. Therefore, CT could be a useful therapeutic agent for AD with protective effects on cognitive impairment.

Keywords

References

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