Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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N-Methyl-D-Aspartate (NMDA) Receptor Antagonistic Effect and Ginsenoside Content of Panax ginseng C. A. Meyer Cultivar Root Extracts

인삼 품종별 뿌리 추출물의 NMDA 수용체 길항 효과 및 진세노사이드 함량

  • 이승은 (국립원예특작과학원 인삼특작부 인삼특작이용팀) ;
  • 김장욱 (국립원예특작과학원 인삼특작부 인삼특작이용팀) ;
  • 정현수 (국립원예특작과학원 인삼특작부 인삼특작이용팀) ;
  • 최재훈 (국립원예특작과학원 인삼특작부 인삼특작이용팀) ;
  • 지윤정 (국립원예특작과학원 인삼특작부 인삼특작이용팀) ;
  • 김형돈 (국립원예특작과학원 인삼특작부 인삼특작이용팀) ;
  • 장귀영 (국립원예특작과학원 인삼특작부 인삼특작이용팀) ;
  • 현동윤 (국립원예특작과학원 인삼특작부 인삼특작이용팀) ;
  • 김동휘 (국립원예특작과학원 인삼특작부 인삼특작이용팀)
  • Received : 2019.10.18
  • Accepted : 2019.12.26
  • Published : 2020.02.28
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Abstract

Background: Although a number of Panax ginseng cultivars have been developed by Korean researchers in recent years, there has been insufficient analysis of their beneficial properties. In this study, we sought to identify useful ginseng varieties as functional materials. Methods and Results: We evaluated effects of root extracts of 10 ginseng cultivars (Cheongsun; CS, Chunpoong; CP, Gopoong; GP, Gumpoong; GMP, K1, Sunhyang; SH, Sunone; SO, Sunpoong; SP, Sunun; SU and Yunpoong; YP) against the inhibitory effects of nitric oxide (NO) and reactive oxygen species (ROS) production in mouse brain microglial BV2 cells, as well as the binding of N-methyl-D-aspartate receptor (NMDAR), a marker related to memory. Ginsenosides, such as 20 (S)-protopanaxadiols (PPDs), including ginsenoside-Rb1, -Rb2, -Rb3, -Rc, -Rd, and - Rg3 and 20 (S)-protopanaxatriols (PPTs) including -Re, -Rg1, and -Rg2 were analyzed by HPLC. We observed that the cultivar GMP showed the highest inhibitory effect (60.8%) against NO production at 20 ㎍/㎖. Those cultivars showing the significantly highest inhibition effects against ROS at 20 ㎍/㎖ were K1 (57.3%), SP (54.5%), YP (53.1%), CP (51.7%), CS (50.9%) and SH (49.6%). At 50 ㎍/㎖, K1 showed the most potent inhibitory effect (51.2%) on NMDAR binding. The total phenol content of SH (1.89 mg/g) and K1 (1.73 mg/g) were higher than those of the other cultivars, whereas in terms of PD/PT ratios, the values of CP (0.98), K1 (1.05) and SO (1.05) were lower than those of the other cultivars. On the basis of correlation coefficient (0.7064) between NMDAR inhibition and ONOO- scavenging activity. Conclusions: The findings of this study indicate that the cultivars K1 and SH could be useful ginseng resources as functional materials with favorable cognition-improving and antioxidative properties.

Keywords

References

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