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Effect of Germination Temperature, Spermine and Putrescine on Pollen Viability of Strawberry

발아온도 및 Spermine, Putrescine이 딸기의 화분 발아에 미치는 영향

  • Lee, Jung-Eun (Department of Horticulture Bioscience, Pusan National University) ;
  • Kim, Hyeon-Do (Department of Horticulture Bioscience, Pusan National University) ;
  • Je, Byoung-Il (Department of Horticulture Bioscience, Pusan National University) ;
  • Lee, Yong-Jae (Department of Horticulture Bioscience, Pusan National University) ;
  • Park, Young-Hoon (Department of Horticulture Bioscience, Pusan National University) ;
  • Choi, Young-Whan (Department of Horticulture Bioscience, Pusan National University) ;
  • Son, Beung-Gu (Department of Horticulture Bioscience, Pusan National University) ;
  • Kang, Nam-Jun (Department of Horticulture, Gyeongsang National University) ;
  • Kang, Jum-Soon (Department of Horticulture Bioscience, Pusan National University)
  • 이정은 (부산대학교 원예생명과학과) ;
  • 김현도 (부산대학교 원예생명과학과) ;
  • 제병일 (부산대학교 원예생명과학과) ;
  • 이용재 (부산대학교 원예생명과학과) ;
  • 박영훈 (부산대학교 원예생명과학과) ;
  • 최영환 (부산대학교 원예생명과학과) ;
  • 손병구 (부산대학교 원예생명과학과) ;
  • 강남준 (경상대학교 원예학과) ;
  • 강점순 (부산대학교 원예생명과학과)
  • Received : 2020.03.27
  • Accepted : 2020.04.10
  • Published : 2020.04.30

Abstract

This study was conducted to determine the optimum conditions for the treatment of polyamine to promote pollen germination and improve the fruiting rate by overcoming fertilization defects in low-temperature strawberry cultivation. The optimum temperature for pollen germination of strawberries was 30 ℃ for Seolhyang and 25 ℃ for Maehyang, and Seolhyang had a higher pollen germination rate than that of Maehyang. The addition of polyamines, namely spermine and putrescine to the medium improved the pollen germination rate and spermine treatment showed a better effect than putrescine treatment. The proper polyamine type and treatment concentration for enhancing the potency of pollen germination was 500 μM of spermine for both Seolhyang and Maehyang, which improved the pollen germination rate by 19-23% compared with that of the control. However, combined treatment of spermine and putrescine, resulted in a lower germination rate lower than that of the single treatment. Our results indicated that the treatment of polyamines during flowering in protected cultivation of strawberrise can improve the fruiting rate by overcoming the problem of poor pollen germination due to low temperature.

Keywords

References

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