DOI QR코드

DOI QR Code

Optimization of Combined Process of Enzymatic Hydrolysis and Solvent Extraction for Production of Lycopene from Elaeagnus umbellata

보리수 나무 열매로부터 라이코펜 생산을 위한 효소 분해 및 유기용매 추출 복합 공정의 최적화

  • Oh, Yun Hye (Department of Food and Biotechnology, Hoseo University) ;
  • Lee, Ju Mi (Department of Food and Biotechnology, Hoseo University) ;
  • Chae, Hee Jeong (Department of Food and Pharmaceutical Engineering, Hoseo University)
  • 오윤혜 (호서대학교 식품생물공학과) ;
  • 이주미 (호서대학교 식품생물공학과) ;
  • 채희정 (호서대학교 식품제약공학부)
  • Received : 2020.01.06
  • Accepted : 2020.04.03
  • Published : 2020.04.29

Abstract

This study was undertaken to optimize combining the processes of enzymatic hydrolysis and extraction for lycopene production from autumn olive berry. The autumn olive berry was pulverized and suspended in water, followed by treatment with various hydrolytic enzymes including Ceremix, Celluclast, AMG, Viscozyme, Pectinex, Promozyme, Ultraflo and Tunicase. Reaction solutions were subjected to extraction by applying different organic solvents including acetone, ethyl acetate, hexane and chloroform. Highest yields of lycopene extraction were obtained with the Ceremix (hydrolysis enzyme) and chloroform (extraction solvent) combination. Subsequently, using this ideal combination, enzymatic hydrolysis conditions, including enzyme concentration, pH and temperature, were statistically optimized to 0.58%, 5.5 and 54.4℃, respectively, by applying the response surface method. The lycopene extraction yield increased 2.3-fold (22.6 mg/100g) by using the selected combined process. We propose that these results could be used for the future development of bioactive materials required for bio-health care products.

보리수 나무 열매로부터 효소 분해 및 유기용매 추출의 복합 공정에 의한 라이코펜의 생산 조건을 최적화하였다. 보리수 나무의 열매를 증류수에 현탁하여 Celluclast, Ceremix, AMG, Viscozyme, Promozyme, Pectinex, Tunicase 및 Ultraflo와 같은 가수분해 효소를 사용하여 효소 반응을 수행하였고 효소 반응액을 에틸아세테이트, 아세톤, 클로로포름 및 헥산과 같은 유기 용매로 추출하였다. 가수분해 효소 중 Ceremix 복합 효소를 사용하고, 추출 용매는 클로로포름을 이용하여 추출하였을 때 가장 높은 lycopene 추출률을 나타냈다. 보리수 나무 열매의 효소처리 조건 최적화를 위해 실험계획법을 이용한 결과, 효소 반응의 pH는 5.5로, 반응 온도는 54.4℃로, 효소 첨가 농도는 0.58%로 하였을 때 lycopene 추출률이 가장 높았고 이 때 lycopene의 함량은 22.6 mg/100g을 나타냈다. 이와 같이 효소적 가수분해 공정과 유기 용매 추출 공정을 결합하여 활용하는 복합 공정은 기존 용매추출법에 비해 유효성분인 lycopene의 수율을 2.3배 증가시켰다. 이는 향후 바이오헬스 케어 제품의 기능성 소재로의 개발에 응용할 수 있는 결과라고 사료된다.

Keywords

References

  1. R. Pei, M. Yu, R. Bruno, B. W. Bolling, "Phenolic and tocopherol content of autumn olive(Elaeagnus umbellate ) berries", Journal of Functional Foods, Vol.16, pp.305-314, 2015. DOI: http://dx.doi.org/10.1016/j.jff.2015.04.028
  2. H. S. Kang, Optimization of Lycopene Extraction Process from Fruit of Autumn Olive and Biological Activity Evaluation, Master's thesis, Hoseo University, Asan, Korea, 2012.
  3. K. F. Khattak, "Free radical scavenging activity, phytochemical composition and nutrient analysis of Elaeagnus umbellata berry", Jounal of Medicinal Plant Research, Vol.6, No.39, pp.5196-5203, 2012. DOI: https://doi.org/10.5897/JMPR11.1128
  4. I. M. Fordham, B. A. Clevidence, E. R. Wiley, R. H. Zimmerman, "Fruit of autumn olive: a rich source of lycopene", Hortscienceh, Vol.36, No.6, pp.1136-1137, 2001. DOI: https://doi.org/10.21273/HORTSCI.36.6.1136
  5. J. G. Cho, S. H. Kim, J. H. Seo, S. Y. Ahn, E. S. Jeong, H. Y. Park, "Novel function of lycopene in vascular endothelial cell", Journal of Life Science, Vol.20, No.7, pp.1093-1099, 2010. DOI: https://doi.org/10.5352/JLS.2010.20.7.1093
  6. S. P. Wolff, Z. Y. Jiang, J. V. Hunt, "Protein glycation and oxidative stress in diabetes mellitus", Free Radical Biology and Medicine, Vol.10, No.5, pp.339-352, 1991. DOI: https://doi.org/10.1016/0891-5849(91)90040-A
  7. I. F. Strati, E. Gogou, V. Oreopoulou, "Enzyme and high pressure assisted extraction of carotenoids from tomato waste", Food and Bioproducts Processing, Vol.94, pp.668-674, 2015. DOI: https://doi.org/10.1016/j.fbp.2014.09.012
  8. S. J. Choi, S. Y. Kim, S. C. Lee, J. M. Lee, I. S. Lee, M. Y. Jung, S. M. Yang, H. J. Chae, "Anti-oxidant and whitening effects of cell lytic enzyme-treated lotus leaf extract", The Korean Society for Biotechnology and Bioengineering, Vol.24, No.6, pp.579-583, 2009.
  9. J. K. Yoo, J. H. Lee, H. Y. Cho, J. G. Kim, "Change of antioxidant activities in carrots (Daucus carota var. sativa ) with enzyme treatment", The Korean Society of Food Science and Nutrition, Vol.42, No.2, pp. 262-267, 2013. DOI: https://doi.org/10.3746/jkfn.2013.42.2.262
  10. H. K. Kim, J. H. Chun, S. J. Kim, "Method development and analysis of carotenoid compositions in various tomatoes", Korean Journal of Environmental Agriculture, Vol.34, No.3, pp.196-203, 2015. DOI: https://doi.org/10.5338/KJEA.2015.34.3.23
  11. Y. H. Oh, Process Optimization of Enzymatic Extraction of Lycopene from Autumn Olive Fruit and its Stabilization by Nano-emulsion, Master's thesis, Hoseo university, Asan, Korea, 2016.
  12. H. M. Sung, S. J. Kim, K. M. Yun, H. J. Jung, T. Y. Kim, J. H. Wee, "Antioxidant activity of soy-sprout extracts prepared by enzyme and ultra high pressure", The Korean Society of Food Science and Nutrition, Vol.43, No.8, pp.1228-1235, 2014. DOI: https://doi.org/10.3746/jkfn.2014.43.8.1228